Highly Fluorescent and Photostable Probe for Long-Term Bacterial Viability Assay Based on Aggregation-Induced Emission
Long‐term tracking of bacterial viability is of great importance for monitoring the viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antibacterials. Most of the conventional viability dyes, h...
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| Veröffentlicht in: | Advanced healthcare materials 2014-01, Vol.3 (1), p.88-96 |
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| creator | Zhao, Engui Hong, Yuning Chen, Sijie Leung, Chris W. T. Chan, Carrie Y. K. Kwok, Ryan T. K. Lam, Jacky W. Y. Tang, Ben Zhong |
| description | Long‐term tracking of bacterial viability is of great importance for monitoring the viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antibacterials. Most of the conventional viability dyes, however, suffer from high toxicity and/or poor photostability, making them unsuitable for long‐term studies. In this work, an aggregation‐induced emission molecule, TPE‐2BA, which can differentiate dead and living bacteria and serve as a highly fluorescent and photostable probe for long‐term viability assay. TPE‐2BA is a cell‐impermeable DNA stain that binds to the groove of double‐stranded DNA. Bacteria with compromised membrane open the access for TPE‐2BA to reach DNA, endowing it with strong emission. The feasibility of using TPE‐2BA for screening effective bactericides is also demonstrated. Plate count experiment reveals that TPE‐2BA poses negligible toxicity to bacteria, indicating that it is an excellent probe for long‐term bacterial viability assay.
Bacteria with compromised membranes open the access for TPE‐2BA, a fluorophore with aggregation‐induced emission characteristics, to approach their DNA. Such interaction selectively lights up dead bacteria. Possessing high brightness, excellent photostability, and appreciable biocompatibility, TPE‐2BA represents a well‐suited candidate for long‐term tracking of bacterial viability and for bactericide screening. |
| doi_str_mv | 10.1002/adhm.201200475 |
| format | Article |
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Bacteria with compromised membranes open the access for TPE‐2BA, a fluorophore with aggregation‐induced emission characteristics, to approach their DNA. Such interaction selectively lights up dead bacteria. Possessing high brightness, excellent photostability, and appreciable biocompatibility, TPE‐2BA represents a well‐suited candidate for long‐term tracking of bacterial viability and for bactericide screening.</description><identifier>ISSN: 2192-2640</identifier><identifier>EISSN: 2192-2659</identifier><identifier>DOI: 10.1002/adhm.201200475</identifier><identifier>PMID: 23814037</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>aggregation-induced emission ; Assaying ; Bacteria ; bacterial viability assay ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biocompatibility ; Biological and medical sciences ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - chemistry ; DNA, Bacterial - metabolism ; Emission ; Escherichia coli - cytology ; Escherichia coli - drug effects ; Escherichia coli - genetics ; fluorescence imaging ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - metabolism ; Fluorescent Dyes - pharmacology ; Fundamental and applied biological sciences. Psychology ; Light ; Microbial Viability ; Microbiology ; Microscopy, Fluorescence ; tetraphenylethene ; Toxicity ; Tracking ; Ultraviolet Rays ; Viability</subject><ispartof>Advanced healthcare materials, 2014-01, Vol.3 (1), p.88-96</ispartof><rights>Copyright © 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6105-12ddc774ed09b5585c30653d3d001788bf398a76bc1ce0a7ef05ecbe2bed70db3</citedby><cites>FETCH-LOGICAL-c6105-12ddc774ed09b5585c30653d3d001788bf398a76bc1ce0a7ef05ecbe2bed70db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadhm.201200475$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadhm.201200475$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28133959$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23814037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Engui</creatorcontrib><creatorcontrib>Hong, Yuning</creatorcontrib><creatorcontrib>Chen, Sijie</creatorcontrib><creatorcontrib>Leung, Chris W. T.</creatorcontrib><creatorcontrib>Chan, Carrie Y. K.</creatorcontrib><creatorcontrib>Kwok, Ryan T. K.</creatorcontrib><creatorcontrib>Lam, Jacky W. Y.</creatorcontrib><creatorcontrib>Tang, Ben Zhong</creatorcontrib><title>Highly Fluorescent and Photostable Probe for Long-Term Bacterial Viability Assay Based on Aggregation-Induced Emission</title><title>Advanced healthcare materials</title><addtitle>Advanced Healthcare Materials</addtitle><description>Long‐term tracking of bacterial viability is of great importance for monitoring the viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antibacterials. Most of the conventional viability dyes, however, suffer from high toxicity and/or poor photostability, making them unsuitable for long‐term studies. In this work, an aggregation‐induced emission molecule, TPE‐2BA, which can differentiate dead and living bacteria and serve as a highly fluorescent and photostable probe for long‐term viability assay. TPE‐2BA is a cell‐impermeable DNA stain that binds to the groove of double‐stranded DNA. Bacteria with compromised membrane open the access for TPE‐2BA to reach DNA, endowing it with strong emission. The feasibility of using TPE‐2BA for screening effective bactericides is also demonstrated. Plate count experiment reveals that TPE‐2BA poses negligible toxicity to bacteria, indicating that it is an excellent probe for long‐term bacterial viability assay.
Bacteria with compromised membranes open the access for TPE‐2BA, a fluorophore with aggregation‐induced emission characteristics, to approach their DNA. Such interaction selectively lights up dead bacteria. Possessing high brightness, excellent photostability, and appreciable biocompatibility, TPE‐2BA represents a well‐suited candidate for long‐term tracking of bacterial viability and for bactericide screening.</description><subject>aggregation-induced emission</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>bacterial viability assay</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biocompatibility</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - metabolism</subject><subject>Emission</subject><subject>Escherichia coli - cytology</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>fluorescence imaging</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fluorescent Dyes - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Light</subject><subject>Microbial Viability</subject><subject>Microbiology</subject><subject>Microscopy, Fluorescence</subject><subject>tetraphenylethene</subject><subject>Toxicity</subject><subject>Tracking</subject><subject>Ultraviolet Rays</subject><subject>Viability</subject><issn>2192-2640</issn><issn>2192-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0c1v0zAYB-AIgdg0duWILCEkLil2HH_kWMq2TpSxwxi7WY79pvVI481OYPnv56qlIC7DF1v28_rrl2WvCZ4QjIsP2q7WkwKTAuNSsGfZYUGqIi84q57vxyU-yI5jvMWpcUa4JC-zg4JKUmIqDrOfc7dctSM6bQcfIBroeqQ7iy5Xvvex13UL6DL4GlDjA1r4bplfQVijj9r0EJxu0bXTtWtdP6JpjHpMKxEs8h2aLpcBlrp3vsvPOzuYNH2ydjGmiVfZi0a3EY53_VH27fTkajbPF1_PzmfTRW44wSwnhbVGiBIsrmrGJDM0PYJaajEmQsq6oZXUgteGGMBaQIMZmBqKGqzAtqZH2fvtvnfB3w8Qe5UuYKBtdQd-iIoIXIn0d0T-DyUbyfnTtKywKKuSlYm-_Yfe-iF06c2KMF6mkLgskppslQk-xgCNugturcOoCFabqNUmarWPOhW82W071Guwe_472ATe7YCORrdN0J1x8Y-ThNKKVclVW_fLtTA-cayafpp_-fsS-bbWxR4e9rU6_FBc0MS_X5ypmby5uZafubqgj60m0C8</recordid><startdate>201401</startdate><enddate>201401</enddate><creator>Zhao, Engui</creator><creator>Hong, Yuning</creator><creator>Chen, Sijie</creator><creator>Leung, Chris W. T.</creator><creator>Chan, Carrie Y. K.</creator><creator>Kwok, Ryan T. K.</creator><creator>Lam, Jacky W. Y.</creator><creator>Tang, Ben Zhong</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T5</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7TO</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>C1K</scope><scope>P64</scope></search><sort><creationdate>201401</creationdate><title>Highly Fluorescent and Photostable Probe for Long-Term Bacterial Viability Assay Based on Aggregation-Induced Emission</title><author>Zhao, Engui ; Hong, Yuning ; Chen, Sijie ; Leung, Chris W. T. ; Chan, Carrie Y. K. ; Kwok, Ryan T. K. ; Lam, Jacky W. 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Psychology</topic><topic>Light</topic><topic>Microbial Viability</topic><topic>Microbiology</topic><topic>Microscopy, Fluorescence</topic><topic>tetraphenylethene</topic><topic>Toxicity</topic><topic>Tracking</topic><topic>Ultraviolet Rays</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Engui</creatorcontrib><creatorcontrib>Hong, Yuning</creatorcontrib><creatorcontrib>Chen, Sijie</creatorcontrib><creatorcontrib>Leung, Chris W. T.</creatorcontrib><creatorcontrib>Chan, Carrie Y. K.</creatorcontrib><creatorcontrib>Kwok, Ryan T. K.</creatorcontrib><creatorcontrib>Lam, Jacky W. 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T.</au><au>Chan, Carrie Y. K.</au><au>Kwok, Ryan T. K.</au><au>Lam, Jacky W. Y.</au><au>Tang, Ben Zhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly Fluorescent and Photostable Probe for Long-Term Bacterial Viability Assay Based on Aggregation-Induced Emission</atitle><jtitle>Advanced healthcare materials</jtitle><addtitle>Advanced Healthcare Materials</addtitle><date>2014-01</date><risdate>2014</risdate><volume>3</volume><issue>1</issue><spage>88</spage><epage>96</epage><pages>88-96</pages><issn>2192-2640</issn><eissn>2192-2659</eissn><abstract>Long‐term tracking of bacterial viability is of great importance for monitoring the viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antibacterials. Most of the conventional viability dyes, however, suffer from high toxicity and/or poor photostability, making them unsuitable for long‐term studies. In this work, an aggregation‐induced emission molecule, TPE‐2BA, which can differentiate dead and living bacteria and serve as a highly fluorescent and photostable probe for long‐term viability assay. TPE‐2BA is a cell‐impermeable DNA stain that binds to the groove of double‐stranded DNA. Bacteria with compromised membrane open the access for TPE‐2BA to reach DNA, endowing it with strong emission. The feasibility of using TPE‐2BA for screening effective bactericides is also demonstrated. Plate count experiment reveals that TPE‐2BA poses negligible toxicity to bacteria, indicating that it is an excellent probe for long‐term bacterial viability assay.
Bacteria with compromised membranes open the access for TPE‐2BA, a fluorophore with aggregation‐induced emission characteristics, to approach their DNA. Such interaction selectively lights up dead bacteria. Possessing high brightness, excellent photostability, and appreciable biocompatibility, TPE‐2BA represents a well‐suited candidate for long‐term tracking of bacterial viability and for bactericide screening.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>23814037</pmid><doi>10.1002/adhm.201200475</doi><tpages>9</tpages></addata></record> |
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| subjects | aggregation-induced emission Assaying Bacteria bacterial viability assay Bacteriological methods and techniques used in bacteriology Bacteriology Biocompatibility Biological and medical sciences Deoxyribonucleic acid DNA DNA, Bacterial - chemistry DNA, Bacterial - metabolism Emission Escherichia coli - cytology Escherichia coli - drug effects Escherichia coli - genetics fluorescence imaging Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fluorescent Dyes - pharmacology Fundamental and applied biological sciences. Psychology Light Microbial Viability Microbiology Microscopy, Fluorescence tetraphenylethene Toxicity Tracking Ultraviolet Rays Viability |
| title | Highly Fluorescent and Photostable Probe for Long-Term Bacterial Viability Assay Based on Aggregation-Induced Emission |
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