Periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway
In the present study, we aimed to explore the effects of periostin, a cell adhesion protein, on chemoresistance in colon cancer cells. Reverse‐transcription polymerase chain reaction and Western blot analyses were employed to detect periostin expression in SW480 and HT‐29 colon cancer cells treated...
Gespeichert in:
| Veröffentlicht in: | Biotechnology and applied biochemistry 2015-05, Vol.62 (3), p.401-406 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 406 |
|---|---|
| container_issue | 3 |
| container_start_page | 401 |
| container_title | Biotechnology and applied biochemistry |
| container_volume | 62 |
| creator | Xiao, Zhi-ming Wang, Xiao-yan Wang, Ai-min |
| description | In the present study, we aimed to explore the effects of periostin, a cell adhesion protein, on chemoresistance in colon cancer cells. Reverse‐transcription polymerase chain reaction and Western blot analyses were employed to detect periostin expression in SW480 and HT‐29 colon cancer cells treated with oxaliplatin or fluorouracil (5‐FU). Small interfering RNA was used to downregulate endogenous periostin. Annexin‐V/propidium iodide staining was performed to analyze the effects of periostin on drug‐induced apoptosis. The results showed that treatment with oxaliplatin or 5‐FU elevated both the mRNA and protein levels of periostin in SW480 and HT‐29 cells. Silencing of periostin significantly (P |
| doi_str_mv | 10.1002/bab.1193 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1709743672</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3719301701</sourcerecordid><originalsourceid>FETCH-LOGICAL-i4513-945331e97d0563124b4d868dbd331f78066fb50bdfab3907e835bdb004981bc83</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhi0EoktB4hegSFy4pDuOv4_bFbQVS6kECImLZScO62423trJtvvvcdRSJC5w8cjvPB6N9SD0GsMJBqjm1tgTjBV5gmaYCiiloPQpmoGUrKQMkyP0IqVrAJBCVs_RUUWJqBgTM7S5ctGHNPi-8H0z1i4V9dptQ3TJp8H0tct5UYcu5HO6xqJ2XZeKYR3D-HNdmHrwezP43A9tTl1xdUE-zhebYZ7GuPf7_HxnhvWtObxEz1rTJffqoR6jbx_ef12el6vPZxfLxar0066loowQ7JRogHGCK2ppI7lsbJPjVkjgvLUMbNMaSxQIJwmzjQWgSmJbS3KM3t3P3cVwM7o06K1P09amd2FMGgtQghIuqv9BMZUYE_JvlCuoMAfCM_r2L_Q6jLHPf86UVFxipUSm3jxQo926Ru-i35p40L_lZKC8B2595w6PfQx6kq6zdD1J16eL06n-4bM5d_fIm7jRXBDB9PfLM_1jeVl9Wn7hekV-ARHyq3E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1689681997</pqid></control><display><type>article</type><title>Periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Xiao, Zhi-ming ; Wang, Xiao-yan ; Wang, Ai-min</creator><creatorcontrib>Xiao, Zhi-ming ; Wang, Xiao-yan ; Wang, Ai-min</creatorcontrib><description>In the present study, we aimed to explore the effects of periostin, a cell adhesion protein, on chemoresistance in colon cancer cells. Reverse‐transcription polymerase chain reaction and Western blot analyses were employed to detect periostin expression in SW480 and HT‐29 colon cancer cells treated with oxaliplatin or fluorouracil (5‐FU). Small interfering RNA was used to downregulate endogenous periostin. Annexin‐V/propidium iodide staining was performed to analyze the effects of periostin on drug‐induced apoptosis. The results showed that treatment with oxaliplatin or 5‐FU elevated both the mRNA and protein levels of periostin in SW480 and HT‐29 cells. Silencing of periostin significantly (P < 0.01) augmented drug‐induced apoptosis in colon cancer cells, coupled with enhanced cleavage of caspase‐3 and poly(ADP‐ribose) polymerase. Mechanistic studies revealed that periostin silencing significantly (P < 0.01) suppressed the expression of survivin, an antiapoptotic protein in colon cancer cells. Enforced expression of survivin repressed drug‐induced apoptosis in periostin‐depleted SW480 and HT‐29 cells. Additionally, periostin overexpression increased the expression of survivin and the phosphorylation of Akt, which was reversed by pretreatment with the phosphatidylinositol 3‐kinase (PI3K)‐specific inhibitor LY294002. Taken together, our data demonstrate that periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1002/bab.1193</identifier><identifier>PMID: 24372557</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Activation ; Apoptosis ; Cancer ; Cell Adhesion Molecules - antagonists & inhibitors ; Cell Adhesion Molecules - genetics ; Cell Adhesion Molecules - metabolism ; Cell Adhesion Molecules - pharmacology ; Cell Line, Tumor ; chemotherapy ; Colon ; Colonic Neoplasms - metabolism ; drug resistance ; Drug Resistance, Neoplasm - drug effects ; Fluorouracil - pharmacology ; Humans ; Inhibitors ; Organoplatinum Compounds - pharmacology ; Pathways ; periostin ; Phosphatidylinositol 3-Kinases - metabolism ; Proteins ; Proto-Oncogene Proteins c-akt - metabolism ; Ribonucleic acids ; Signal Transduction - drug effects ; signaling pathway</subject><ispartof>Biotechnology and applied biochemistry, 2015-05, Vol.62 (3), p.401-406</ispartof><rights>2013 International Union of Biochemistry and Molecular Biology, Inc.</rights><rights>Copyright © 2015 International Union of Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbab.1193$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbab.1193$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24372557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiao, Zhi-ming</creatorcontrib><creatorcontrib>Wang, Xiao-yan</creatorcontrib><creatorcontrib>Wang, Ai-min</creatorcontrib><title>Periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnology and Applied Biochemistry</addtitle><description>In the present study, we aimed to explore the effects of periostin, a cell adhesion protein, on chemoresistance in colon cancer cells. Reverse‐transcription polymerase chain reaction and Western blot analyses were employed to detect periostin expression in SW480 and HT‐29 colon cancer cells treated with oxaliplatin or fluorouracil (5‐FU). Small interfering RNA was used to downregulate endogenous periostin. Annexin‐V/propidium iodide staining was performed to analyze the effects of periostin on drug‐induced apoptosis. The results showed that treatment with oxaliplatin or 5‐FU elevated both the mRNA and protein levels of periostin in SW480 and HT‐29 cells. Silencing of periostin significantly (P < 0.01) augmented drug‐induced apoptosis in colon cancer cells, coupled with enhanced cleavage of caspase‐3 and poly(ADP‐ribose) polymerase. Mechanistic studies revealed that periostin silencing significantly (P < 0.01) suppressed the expression of survivin, an antiapoptotic protein in colon cancer cells. Enforced expression of survivin repressed drug‐induced apoptosis in periostin‐depleted SW480 and HT‐29 cells. Additionally, periostin overexpression increased the expression of survivin and the phosphorylation of Akt, which was reversed by pretreatment with the phosphatidylinositol 3‐kinase (PI3K)‐specific inhibitor LY294002. Taken together, our data demonstrate that periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway.</description><subject>Activation</subject><subject>Apoptosis</subject><subject>Cancer</subject><subject>Cell Adhesion Molecules - antagonists & inhibitors</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Adhesion Molecules - pharmacology</subject><subject>Cell Line, Tumor</subject><subject>chemotherapy</subject><subject>Colon</subject><subject>Colonic Neoplasms - metabolism</subject><subject>drug resistance</subject><subject>Drug Resistance, Neoplasm - drug effects</subject><subject>Fluorouracil - pharmacology</subject><subject>Humans</subject><subject>Inhibitors</subject><subject>Organoplatinum Compounds - pharmacology</subject><subject>Pathways</subject><subject>periostin</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Ribonucleic acids</subject><subject>Signal Transduction - drug effects</subject><subject>signaling pathway</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EoktB4hegSFy4pDuOv4_bFbQVS6kECImLZScO62423trJtvvvcdRSJC5w8cjvPB6N9SD0GsMJBqjm1tgTjBV5gmaYCiiloPQpmoGUrKQMkyP0IqVrAJBCVs_RUUWJqBgTM7S5ctGHNPi-8H0z1i4V9dptQ3TJp8H0tct5UYcu5HO6xqJ2XZeKYR3D-HNdmHrwezP43A9tTl1xdUE-zhebYZ7GuPf7_HxnhvWtObxEz1rTJffqoR6jbx_ef12el6vPZxfLxar0066loowQ7JRogHGCK2ppI7lsbJPjVkjgvLUMbNMaSxQIJwmzjQWgSmJbS3KM3t3P3cVwM7o06K1P09amd2FMGgtQghIuqv9BMZUYE_JvlCuoMAfCM_r2L_Q6jLHPf86UVFxipUSm3jxQo926Ru-i35p40L_lZKC8B2595w6PfQx6kq6zdD1J16eL06n-4bM5d_fIm7jRXBDB9PfLM_1jeVl9Wn7hekV-ARHyq3E</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>Xiao, Zhi-ming</creator><creator>Wang, Xiao-yan</creator><creator>Wang, Ai-min</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201505</creationdate><title>Periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway</title><author>Xiao, Zhi-ming ; Wang, Xiao-yan ; Wang, Ai-min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i4513-945331e97d0563124b4d868dbd331f78066fb50bdfab3907e835bdb004981bc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Activation</topic><topic>Apoptosis</topic><topic>Cancer</topic><topic>Cell Adhesion Molecules - antagonists & inhibitors</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Adhesion Molecules - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>chemotherapy</topic><topic>Colon</topic><topic>Colonic Neoplasms - metabolism</topic><topic>drug resistance</topic><topic>Drug Resistance, Neoplasm - drug effects</topic><topic>Fluorouracil - pharmacology</topic><topic>Humans</topic><topic>Inhibitors</topic><topic>Organoplatinum Compounds - pharmacology</topic><topic>Pathways</topic><topic>periostin</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Ribonucleic acids</topic><topic>Signal Transduction - drug effects</topic><topic>signaling pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiao, Zhi-ming</creatorcontrib><creatorcontrib>Wang, Xiao-yan</creatorcontrib><creatorcontrib>Wang, Ai-min</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiao, Zhi-ming</au><au>Wang, Xiao-yan</au><au>Wang, Ai-min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><addtitle>Biotechnology and Applied Biochemistry</addtitle><date>2015-05</date><risdate>2015</risdate><volume>62</volume><issue>3</issue><spage>401</spage><epage>406</epage><pages>401-406</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><abstract>In the present study, we aimed to explore the effects of periostin, a cell adhesion protein, on chemoresistance in colon cancer cells. Reverse‐transcription polymerase chain reaction and Western blot analyses were employed to detect periostin expression in SW480 and HT‐29 colon cancer cells treated with oxaliplatin or fluorouracil (5‐FU). Small interfering RNA was used to downregulate endogenous periostin. Annexin‐V/propidium iodide staining was performed to analyze the effects of periostin on drug‐induced apoptosis. The results showed that treatment with oxaliplatin or 5‐FU elevated both the mRNA and protein levels of periostin in SW480 and HT‐29 cells. Silencing of periostin significantly (P < 0.01) augmented drug‐induced apoptosis in colon cancer cells, coupled with enhanced cleavage of caspase‐3 and poly(ADP‐ribose) polymerase. Mechanistic studies revealed that periostin silencing significantly (P < 0.01) suppressed the expression of survivin, an antiapoptotic protein in colon cancer cells. Enforced expression of survivin repressed drug‐induced apoptosis in periostin‐depleted SW480 and HT‐29 cells. Additionally, periostin overexpression increased the expression of survivin and the phosphorylation of Akt, which was reversed by pretreatment with the phosphatidylinositol 3‐kinase (PI3K)‐specific inhibitor LY294002. Taken together, our data demonstrate that periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24372557</pmid><doi>10.1002/bab.1193</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0885-4513 |
| ispartof | Biotechnology and applied biochemistry, 2015-05, Vol.62 (3), p.401-406 |
| issn | 0885-4513 1470-8744 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1709743672 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Activation Apoptosis Cancer Cell Adhesion Molecules - antagonists & inhibitors Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism Cell Adhesion Molecules - pharmacology Cell Line, Tumor chemotherapy Colon Colonic Neoplasms - metabolism drug resistance Drug Resistance, Neoplasm - drug effects Fluorouracil - pharmacology Humans Inhibitors Organoplatinum Compounds - pharmacology Pathways periostin Phosphatidylinositol 3-Kinases - metabolism Proteins Proto-Oncogene Proteins c-akt - metabolism Ribonucleic acids Signal Transduction - drug effects signaling pathway |
| title | Periostin induces chemoresistance in colon cancer cells through activation of the PI3K/Akt/survivin pathway |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T02%3A12%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Periostin%20induces%20chemoresistance%20in%20colon%20cancer%20cells%20through%20activation%20of%20the%20PI3K/Akt/survivin%20pathway&rft.jtitle=Biotechnology%20and%20applied%20biochemistry&rft.au=Xiao,%20Zhi-ming&rft.date=2015-05&rft.volume=62&rft.issue=3&rft.spage=401&rft.epage=406&rft.pages=401-406&rft.issn=0885-4513&rft.eissn=1470-8744&rft_id=info:doi/10.1002/bab.1193&rft_dat=%3Cproquest_pubme%3E3719301701%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1689681997&rft_id=info:pmid/24372557&rfr_iscdi=true |