Evaluation of characteristics and in vitro antioxidant properties of RSV loaded hyaluronic acid–DPPC microparticles as a wound healing system

•RSV loaded HA–DPPC microparticles performed a controlled and dose-dependent release.•RSV encapsulation enhanced enzymatic degradation by a possible change in DPPC structure.•There was no significant difference between groups in terms of their MDA, SOD and GPX levels.•GSH/GSSG levels acted as an evidence of the radical scavenging properties of RSV.•The components of microparticle matrix affected human dermal fibroblast cell proliferation in a synergistic way. Resveratrol (RSV) was incorporated into microparticles by spray drying to treat chronic wounds such as diabetic ulcers. RSV was chosen due to its defense mechanisms as the formation of free radicals delays the healing process. RSV was loaded into microparticles consisting of dipalmitoylphosphatidylcholine (DPPC) and hyaluronic acid (HA), a polysaccharide naturally present within the skin, known to contribute to the healing process. Microparticles were evaluated in terms of production yield, size distribution, encapsulation efficiency, morphology, specific surface area, thermal properties and water content. Spherical and homogenous microparticles (span≤2) in a size range between 20 and 30μm were obtained with high encapsulation efficiency (≥97%). The effect of enzymes (hyaluronidase, phospholipase and lipase) on RSV release showed a dose-dependent pattern followed by a slow release stage. Cytotoxicity/proliferation and oxidative stress parameters (glutathione, oxidized glutathione, glutathione peroxidase, malondialdehyde, superoxide dismutase) obtained from human dermal fibroblast cell cultures revealed that formulations increased cell proliferation and the presence of RSV decreased oxidation in cells. RSV-loaded HA–DPPC microparticles appear as a promising formulation for wound healing due to synergistic effect of the ingredients.