Temporal and spatial expression of flavonoid biosynthetic genes in flowers of Anthurium andraeanum
The expression of anthocyanin biosynthesis genes during flower colour development in Anthurium andraeanum (anthurium) was studied. A cDNA library was constructed from mRNA from the anthurium spathe, and full‐length cDNA clones identified for the flavonoid biosynthetic enzymes chalcone synthase (CHS)...
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| Veröffentlicht in: | Physiologia plantarum 2004-11, Vol.122 (3), p.297-304 |
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| container_title | Physiologia plantarum |
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| creator | Collette, Vern E. Jameson, Paula E. Schwinn, Kathy E. Umaharan, Pathmanathan Davies, Kevin M. |
| description | The expression of anthocyanin biosynthesis genes during flower colour development in Anthurium andraeanum (anthurium) was studied. A cDNA library was constructed from mRNA from the anthurium spathe, and full‐length cDNA clones identified for the flavonoid biosynthetic enzymes chalcone synthase (CHS), flavanone 3‐hydroxylase (F3H), dihydroflavonol 4‐reductase (DFR) and anthocyanidin synthase (ANS). These were used to measure transcript levels in the spathe during flower development, in cultivars with different flower colours, over the diurnal cycle, and in the spadix. CHS, F3H and ANS were expressed at all stages of spathe and spadix development. However, DFR transcript levels varied significantly between stages, and DFR may represent a key point of regulation. A diurnal rhythm of DFR transcript abundance in the spathe was also observed, with transcript levels high at dawn and dusk and low at noon. Control of anthocyanin biosynthesis in anthurium spathe differs from that described for flowers of other species, with DFR a key regulatory point and a complex mix of developmental and environmental control signals. |
| doi_str_mv | 10.1111/j.1399-3054.2004.00402.x |
| format | Article |
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A cDNA library was constructed from mRNA from the anthurium spathe, and full‐length cDNA clones identified for the flavonoid biosynthetic enzymes chalcone synthase (CHS), flavanone 3‐hydroxylase (F3H), dihydroflavonol 4‐reductase (DFR) and anthocyanidin synthase (ANS). These were used to measure transcript levels in the spathe during flower development, in cultivars with different flower colours, over the diurnal cycle, and in the spadix. CHS, F3H and ANS were expressed at all stages of spathe and spadix development. However, DFR transcript levels varied significantly between stages, and DFR may represent a key point of regulation. A diurnal rhythm of DFR transcript abundance in the spathe was also observed, with transcript levels high at dawn and dusk and low at noon. 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A cDNA library was constructed from mRNA from the anthurium spathe, and full‐length cDNA clones identified for the flavonoid biosynthetic enzymes chalcone synthase (CHS), flavanone 3‐hydroxylase (F3H), dihydroflavonol 4‐reductase (DFR) and anthocyanidin synthase (ANS). These were used to measure transcript levels in the spathe during flower development, in cultivars with different flower colours, over the diurnal cycle, and in the spadix. CHS, F3H and ANS were expressed at all stages of spathe and spadix development. However, DFR transcript levels varied significantly between stages, and DFR may represent a key point of regulation. A diurnal rhythm of DFR transcript abundance in the spathe was also observed, with transcript levels high at dawn and dusk and low at noon. Control of anthocyanin biosynthesis in anthurium spathe differs from that described for flowers of other species, with DFR a key regulatory point and a complex mix of developmental and environmental control signals.</description><subject>Anthurium</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Gene expression</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collette, Vern E.</creatorcontrib><creatorcontrib>Jameson, Paula E.</creatorcontrib><creatorcontrib>Schwinn, Kathy E.</creatorcontrib><creatorcontrib>Umaharan, Pathmanathan</creatorcontrib><creatorcontrib>Davies, Kevin M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Collette, Vern E.</au><au>Jameson, Paula E.</au><au>Schwinn, Kathy E.</au><au>Umaharan, Pathmanathan</au><au>Davies, Kevin M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal and spatial expression of flavonoid biosynthetic genes in flowers of Anthurium andraeanum</atitle><jtitle>Physiologia plantarum</jtitle><date>2004-11</date><risdate>2004</risdate><volume>122</volume><issue>3</issue><spage>297</spage><epage>304</epage><pages>297-304</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><coden>PHPLAI</coden><abstract>The expression of anthocyanin biosynthesis genes during flower colour development in Anthurium andraeanum (anthurium) was studied. A cDNA library was constructed from mRNA from the anthurium spathe, and full‐length cDNA clones identified for the flavonoid biosynthetic enzymes chalcone synthase (CHS), flavanone 3‐hydroxylase (F3H), dihydroflavonol 4‐reductase (DFR) and anthocyanidin synthase (ANS). These were used to measure transcript levels in the spathe during flower development, in cultivars with different flower colours, over the diurnal cycle, and in the spadix. CHS, F3H and ANS were expressed at all stages of spathe and spadix development. However, DFR transcript levels varied significantly between stages, and DFR may represent a key point of regulation. A diurnal rhythm of DFR transcript abundance in the spathe was also observed, with transcript levels high at dawn and dusk and low at noon. Control of anthocyanin biosynthesis in anthurium spathe differs from that described for flowers of other species, with DFR a key regulatory point and a complex mix of developmental and environmental control signals.</abstract><cop>Oxford, UK; Malden, USA</cop><pub>Munksgaard International Publishers</pub><doi>10.1111/j.1399-3054.2004.00402.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Physiologia plantarum, 2004-11, Vol.122 (3), p.297-304 |
| issn | 0031-9317 1399-3054 |
| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Anthurium Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene expression Molecular and cellular biology Molecular genetics |
| title | Temporal and spatial expression of flavonoid biosynthetic genes in flowers of Anthurium andraeanum |
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