Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation
Micronuclei are small extranuclear bodies resulting from chromosome fragments or the whole chromosomes secluded from daughter nuclei during mitosis. The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing r...
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| Veröffentlicht in: | Radiation and environmental biophysics 2015-08, Vol.54 (3), p.327-334 |
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| creator | Litvinchuk, Alexandra V. Vachelová, J. Michaelidesová, A. Wagner, R. Davídková, M. |
| description | Micronuclei are small extranuclear bodies resulting from chromosome fragments or the whole chromosomes secluded from daughter nuclei during mitosis. The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. In case of human exposure to mixed radiation fields or high LET radiation, the proportion of binuclear cells containing micronuclei from all binuclear cells can thus serve as a good biomarker of radiation-induced DNA damage. |
| doi_str_mv | 10.1007/s00411-015-0598-1 |
| format | Article |
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The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. 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The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. 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radiation effects</subject><subject>Micronucleus Tests</subject><subject>Monitoring/Environmental Analysis</subject><subject>Original Paper</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><subject>Protons</subject><subject>Protons - adverse effects</subject><subject>Radiation</subject><issn>0301-634X</issn><issn>1432-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkU9rFjEQxoMo9rX6AbyUgBcvaWeSTbI5Sv1XKHhR8CCEbJJXt-wm22QX6rc3r28LxZOnmWF-8zAzDyGvEc4RQF9UgA6RAUoG0vQMn5AddoIzDsY8JTsQgEyJ7vsJeVHrDQBqpcxzcsKl0ZwrvSM_3ucaWYhLTCGmlc6jLzltfooj3ecyu3XMiY6JpkMx0V_b7BLdj0PJw-TqWmm8W5pEoGumS8lro4sL49-5l-TZ3k01vrqPp-Tbxw9fLz-z6y-fri7fXbOFd3JlGpyWHI0DwbU2Ao3ygbdUGK_04D3wTvk-YBwiBvRib4QcvJS9jMGBFqfk7VG3LXC7xbraeaw-TpNLMW_VogaDfYfiP1BlemE4R2jom3_Qm7yV1A45UFpp1d7fqLN7ahvmGOxSxtmV3_bhxQ3gR6C2VvoZyyMZsAcf7dFH23y0Bx8tij_uCY0d</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Litvinchuk, Alexandra V.</creator><creator>Vachelová, J.</creator><creator>Michaelidesová, A.</creator><creator>Wagner, R.</creator><creator>Davídková, M.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7ST</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20150801</creationdate><title>Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation</title><author>Litvinchuk, Alexandra V. ; Vachelová, J. ; Michaelidesová, A. ; Wagner, R. ; Davídková, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p245t-70a75219a0327793196cd227739c67bcc0246c8d1ebe1d1c3f935bc5585eda073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biological and Medical Physics</topic><topic>Biology</topic><topic>Biophysics</topic><topic>Cell Line</topic><topic>Chromosome aberrations</topic><topic>Chromosomes</topic><topic>Cobalt</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Ecosystems</topic><topic>Effects of Radiation/Radiation Protection</topic><topic>Environmental Physics</topic><topic>Fibroblasts - 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Academic</collection><jtitle>Radiation and environmental biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Litvinchuk, Alexandra V.</au><au>Vachelová, J.</au><au>Michaelidesová, A.</au><au>Wagner, R.</au><au>Davídková, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation</atitle><jtitle>Radiation and environmental biophysics</jtitle><stitle>Radiat Environ Biophys</stitle><addtitle>Radiat Environ Biophys</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>54</volume><issue>3</issue><spage>327</spage><epage>334</epage><pages>327-334</pages><issn>0301-634X</issn><eissn>1432-2099</eissn><abstract>Micronuclei are small extranuclear bodies resulting from chromosome fragments or the whole chromosomes secluded from daughter nuclei during mitosis. The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. In case of human exposure to mixed radiation fields or high LET radiation, the proportion of binuclear cells containing micronuclei from all binuclear cells can thus serve as a good biomarker of radiation-induced DNA damage.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25972267</pmid><doi>10.1007/s00411-015-0598-1</doi><tpages>8</tpages></addata></record> |
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| subjects | Biological and Medical Physics Biology Biophysics Cell Line Chromosome aberrations Chromosomes Cobalt Deoxyribonucleic acid DNA DNA Damage Dose-Response Relationship, Radiation Ecosystems Effects of Radiation/Radiation Protection Environmental Physics Fibroblasts - radiation effects Gamma rays Gamma Rays - adverse effects Humans Ionizing radiation Linear Energy Transfer Micronuclei, Chromosome-Defective - radiation effects Micronucleus Tests Monitoring/Environmental Analysis Original Paper Physics Physics and Astronomy Protons Protons - adverse effects Radiation |
| title | Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation |
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