Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta)
A novel endophytic actinomycete, strain NEAU-TX1-15ᵀ, was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15ᵀ. Morphological and chemotaxonomic properties of strain NEAU-TX1-15ᵀ ar...
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2015-08, Vol.108 (2), p.453-459 |
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| creator | Li, Chuang Wang, Haiyan Jin, Pinjiao Zheng, Weijia Chu, Liyang Liu, Chongxi Li, Jiansong Xiang, Wensheng Wang, Xiangjing |
| description | A novel endophytic actinomycete, strain NEAU-TX1-15ᵀ, was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15ᵀ. Morphological and chemotaxonomic properties of strain NEAU-TX1-15ᵀ are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15ᵀ was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H₆) and MK-9(H₈). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C₁₆:₀. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686ᵀ. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15ᵀ could also be differentiated from A. yoronensis NBRC 103686ᵀ and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA–DNA hybridization value, indicated that strain NEAU-TX1-15ᵀ represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15ᵀ (=CGMCC 4.7200ᵀ = JCM 30340ᵀ). |
| doi_str_mv | 10.1007/s10482-015-0498-z |
| format | Article |
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A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15ᵀ. Morphological and chemotaxonomic properties of strain NEAU-TX1-15ᵀ are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15ᵀ was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H₆) and MK-9(H₈). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C₁₆:₀. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686ᵀ. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15ᵀ could also be differentiated from A. yoronensis NBRC 103686ᵀ and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA–DNA hybridization value, indicated that strain NEAU-TX1-15ᵀ represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15ᵀ (=CGMCC 4.7200ᵀ = JCM 30340ᵀ).</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-015-0498-z</identifier><identifier>PMID: 26033369</identifier><language>eng</language><publisher>Netherlands: Springer International Publishing</publisher><subject>Actinobacteria - classification ; Actinobacteria - genetics ; Actinobacteria - isolation & purification ; Actinobacteria - physiology ; Bacterial Typing Techniques ; Bryophyta ; Bryophyta - microbiology ; Bryopsida ; Cell Wall - chemistry ; cell walls ; chemotaxonomy ; China ; Cluster Analysis ; Cytosol - chemistry ; Diaminopimelic Acid - analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; endophytes ; Endophytes - classification ; Endophytes - genetics ; Endophytes - isolation & purification ; Endophytes - physiology ; fatty acids ; Fatty Acids - analysis ; hyphae ; lysine ; menaquinones ; Microscopy, Electron, Scanning ; Molecular Sequence Data ; mosses and liverworts ; new species ; Nucleic Acid Hybridization ; nucleotide sequences ; Peptidoglycan - analysis ; peptidoglycans ; phospholipids ; Phospholipids - analysis ; Phylogeny ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; sequence homology ; spores ; Spores, Bacterial - cytology ; Vitamin K 2 - analysis</subject><ispartof>Antonie van Leeuwenhoek, 2015-08, Vol.108 (2), p.453-459</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-68e52ba7cd8f26c9f9e640602ce47621bfacc307faf6e21511d5b50eda56ad923</citedby><cites>FETCH-LOGICAL-c358t-68e52ba7cd8f26c9f9e640602ce47621bfacc307faf6e21511d5b50eda56ad923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26033369$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Chuang</creatorcontrib><creatorcontrib>Wang, Haiyan</creatorcontrib><creatorcontrib>Jin, Pinjiao</creatorcontrib><creatorcontrib>Zheng, Weijia</creatorcontrib><creatorcontrib>Chu, Liyang</creatorcontrib><creatorcontrib>Liu, Chongxi</creatorcontrib><creatorcontrib>Li, Jiansong</creatorcontrib><creatorcontrib>Xiang, Wensheng</creatorcontrib><creatorcontrib>Wang, Xiangjing</creatorcontrib><title>Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta)</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie Van Leeuwenhoek</addtitle><description>A novel endophytic actinomycete, strain NEAU-TX1-15ᵀ, was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15ᵀ. Morphological and chemotaxonomic properties of strain NEAU-TX1-15ᵀ are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15ᵀ was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H₆) and MK-9(H₈). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C₁₆:₀. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686ᵀ. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15ᵀ could also be differentiated from A. yoronensis NBRC 103686ᵀ and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA–DNA hybridization value, indicated that strain NEAU-TX1-15ᵀ represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15ᵀ (=CGMCC 4.7200ᵀ = JCM 30340ᵀ).</description><subject>Actinobacteria - classification</subject><subject>Actinobacteria - genetics</subject><subject>Actinobacteria - isolation & purification</subject><subject>Actinobacteria - physiology</subject><subject>Bacterial Typing Techniques</subject><subject>Bryophyta</subject><subject>Bryophyta - microbiology</subject><subject>Bryopsida</subject><subject>Cell Wall - chemistry</subject><subject>cell walls</subject><subject>chemotaxonomy</subject><subject>China</subject><subject>Cluster Analysis</subject><subject>Cytosol - chemistry</subject><subject>Diaminopimelic Acid - analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>endophytes</subject><subject>Endophytes - classification</subject><subject>Endophytes - genetics</subject><subject>Endophytes - isolation & purification</subject><subject>Endophytes - physiology</subject><subject>fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>hyphae</subject><subject>lysine</subject><subject>menaquinones</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molecular Sequence Data</subject><subject>mosses and liverworts</subject><subject>new species</subject><subject>Nucleic Acid Hybridization</subject><subject>nucleotide sequences</subject><subject>Peptidoglycan - analysis</subject><subject>peptidoglycans</subject><subject>phospholipids</subject><subject>Phospholipids - analysis</subject><subject>Phylogeny</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>sequence homology</subject><subject>spores</subject><subject>Spores, Bacterial - cytology</subject><subject>Vitamin K 2 - analysis</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1OxCAURonR6Dj6AG6UpSZ2vECBslTjXzKJC3WNlILWtGWE1mR8eqszunZFbjjfSe79EDogMCMA8iwRyAuaAeEZ5KrIPjfQhHBJMyWU2kQTAGCZAEl30G5Kb-OoRCG30Q4VwBgTaoKez21fd8E0TWiHOCRcxmVYvC77EIcWp8UMd-FjdopNh11X_fzUFpufULu0rne4TqExvauwj6HFbUgJH1-sLeZkD2150yS3v36n6On66vHyNpvf39xdns8zy3jRZ6JwnJZG2qrwVFjllRM5CKDW5VJQUnpjLQPpjReOEk5IxUsOrjJcmEpRNkXHK-8ihvfBpV63dbKuaUznwpA0kaBIwST9BypULkEISUaUrFAbx72i83oR69bEpSagvzvQqw702IH-7kB_jpnDtX4oW1f9JX6PPgJHK8CboM1LrJN-eqCjAYBS4IqzL4UIjKE</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Li, Chuang</creator><creator>Wang, Haiyan</creator><creator>Jin, Pinjiao</creator><creator>Zheng, Weijia</creator><creator>Chu, Liyang</creator><creator>Liu, Chongxi</creator><creator>Li, Jiansong</creator><creator>Xiang, Wensheng</creator><creator>Wang, Xiangjing</creator><general>Springer International Publishing</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20150801</creationdate><title>Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta)</title><author>Li, Chuang ; Wang, Haiyan ; Jin, Pinjiao ; Zheng, Weijia ; Chu, Liyang ; Liu, Chongxi ; Li, Jiansong ; Xiang, Wensheng ; Wang, Xiangjing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-68e52ba7cd8f26c9f9e640602ce47621bfacc307faf6e21511d5b50eda56ad923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Actinobacteria - classification</topic><topic>Actinobacteria - genetics</topic><topic>Actinobacteria - isolation & purification</topic><topic>Actinobacteria - physiology</topic><topic>Bacterial Typing Techniques</topic><topic>Bryophyta</topic><topic>Bryophyta - microbiology</topic><topic>Bryopsida</topic><topic>Cell Wall - chemistry</topic><topic>cell walls</topic><topic>chemotaxonomy</topic><topic>China</topic><topic>Cluster Analysis</topic><topic>Cytosol - chemistry</topic><topic>Diaminopimelic Acid - analysis</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>endophytes</topic><topic>Endophytes - classification</topic><topic>Endophytes - genetics</topic><topic>Endophytes - isolation & purification</topic><topic>Endophytes - physiology</topic><topic>fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>hyphae</topic><topic>lysine</topic><topic>menaquinones</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molecular Sequence Data</topic><topic>mosses and liverworts</topic><topic>new species</topic><topic>Nucleic Acid Hybridization</topic><topic>nucleotide sequences</topic><topic>Peptidoglycan - analysis</topic><topic>peptidoglycans</topic><topic>phospholipids</topic><topic>Phospholipids - analysis</topic><topic>Phylogeny</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>sequence homology</topic><topic>spores</topic><topic>Spores, Bacterial - cytology</topic><topic>Vitamin K 2 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Chuang</creatorcontrib><creatorcontrib>Wang, Haiyan</creatorcontrib><creatorcontrib>Jin, Pinjiao</creatorcontrib><creatorcontrib>Zheng, Weijia</creatorcontrib><creatorcontrib>Chu, Liyang</creatorcontrib><creatorcontrib>Liu, Chongxi</creatorcontrib><creatorcontrib>Li, Jiansong</creatorcontrib><creatorcontrib>Xiang, Wensheng</creatorcontrib><creatorcontrib>Wang, Xiangjing</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Chuang</au><au>Wang, Haiyan</au><au>Jin, Pinjiao</au><au>Zheng, Weijia</au><au>Chu, Liyang</au><au>Liu, Chongxi</au><au>Li, Jiansong</au><au>Xiang, Wensheng</au><au>Wang, Xiangjing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta)</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>108</volume><issue>2</issue><spage>453</spage><epage>459</epage><pages>453-459</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>A novel endophytic actinomycete, strain NEAU-TX1-15ᵀ, was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15ᵀ. Morphological and chemotaxonomic properties of strain NEAU-TX1-15ᵀ are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15ᵀ was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H₆) and MK-9(H₈). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C₁₆:₀. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686ᵀ. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15ᵀ could also be differentiated from A. yoronensis NBRC 103686ᵀ and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA–DNA hybridization value, indicated that strain NEAU-TX1-15ᵀ represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15ᵀ (=CGMCC 4.7200ᵀ = JCM 30340ᵀ).</abstract><cop>Netherlands</cop><pub>Springer International Publishing</pub><pmid>26033369</pmid><doi>10.1007/s10482-015-0498-z</doi><tpages>7</tpages></addata></record> |
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| subjects | Actinobacteria - classification Actinobacteria - genetics Actinobacteria - isolation & purification Actinobacteria - physiology Bacterial Typing Techniques Bryophyta Bryophyta - microbiology Bryopsida Cell Wall - chemistry cell walls chemotaxonomy China Cluster Analysis Cytosol - chemistry Diaminopimelic Acid - analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics endophytes Endophytes - classification Endophytes - genetics Endophytes - isolation & purification Endophytes - physiology fatty acids Fatty Acids - analysis hyphae lysine menaquinones Microscopy, Electron, Scanning Molecular Sequence Data mosses and liverworts new species Nucleic Acid Hybridization nucleotide sequences Peptidoglycan - analysis peptidoglycans phospholipids Phospholipids - analysis Phylogeny ribosomal RNA RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA sequence homology spores Spores, Bacterial - cytology Vitamin K 2 - analysis |
| title | Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta) |
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