Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock
Alkaline pretreatment using NaOH, KOH, or NaOCl has been applied to various types of waste biomass to enhance enzymatic digestibility. Pretreatment (2% NaOH, 121°C, 30min) of rice paddy straw (PS) resulted in a maximum yield of 703mg of reducing sugar per gram of PS with 84.19% hydrolysis yield afte...
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| Veröffentlicht in: | International journal of biological macromolecules 2015-09, Vol.80, p.627-635 |
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| creator | Saratale, Ganesh D. Oh, Min-Kyu |
| description | Alkaline pretreatment using NaOH, KOH, or NaOCl has been applied to various types of waste biomass to enhance enzymatic digestibility. Pretreatment (2% NaOH, 121°C, 30min) of rice paddy straw (PS) resulted in a maximum yield of 703mg of reducing sugar per gram of PS with 84.19% hydrolysis yield after a two-step enzymatic hydrolysis process. Ralstonia eutropha ATCC 17699 was tested for its ability to synthesize poly-3-hydroxybutyrate (PHB) using PS hydrolysates as its sole carbon source. It is noteworthy that dry cell weight, polyhydroxyalkanoate (PHA) accumulation and PHB yield with the use of laboratory-grade sugars were similar to those achieved with PS-derived sugars. Under optimized conditions, we observed maximal PHA accumulation (75.45%) and PHB production (11.42g/L) within 48h of fermentation. After PHB recovery, the physicochemical properties of PHB were determined by various analytical techniques, showed the results were consistent with the characteristics of a standard polymer of PHB. Thus, the PS hydrolysate proved to be an excellent cheap carbon substrate for PHB production. |
| doi_str_mv | 10.1016/j.ijbiomac.2015.07.034 |
| format | Article |
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Pretreatment (2% NaOH, 121°C, 30min) of rice paddy straw (PS) resulted in a maximum yield of 703mg of reducing sugar per gram of PS with 84.19% hydrolysis yield after a two-step enzymatic hydrolysis process. Ralstonia eutropha ATCC 17699 was tested for its ability to synthesize poly-3-hydroxybutyrate (PHB) using PS hydrolysates as its sole carbon source. It is noteworthy that dry cell weight, polyhydroxyalkanoate (PHA) accumulation and PHB yield with the use of laboratory-grade sugars were similar to those achieved with PS-derived sugars. Under optimized conditions, we observed maximal PHA accumulation (75.45%) and PHB production (11.42g/L) within 48h of fermentation. After PHB recovery, the physicochemical properties of PHB were determined by various analytical techniques, showed the results were consistent with the characteristics of a standard polymer of PHB. 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Pretreatment (2% NaOH, 121°C, 30min) of rice paddy straw (PS) resulted in a maximum yield of 703mg of reducing sugar per gram of PS with 84.19% hydrolysis yield after a two-step enzymatic hydrolysis process. Ralstonia eutropha ATCC 17699 was tested for its ability to synthesize poly-3-hydroxybutyrate (PHB) using PS hydrolysates as its sole carbon source. It is noteworthy that dry cell weight, polyhydroxyalkanoate (PHA) accumulation and PHB yield with the use of laboratory-grade sugars were similar to those achieved with PS-derived sugars. Under optimized conditions, we observed maximal PHA accumulation (75.45%) and PHB production (11.42g/L) within 48h of fermentation. After PHB recovery, the physicochemical properties of PHB were determined by various analytical techniques, showed the results were consistent with the characteristics of a standard polymer of PHB. Thus, the PS hydrolysate proved to be an excellent cheap carbon substrate for PHB production.</description><subject>Alkalies - pharmacology</subject><subject>Alkaline pretreatment</subject><subject>Biomass</subject><subject>Carbohydrate Metabolism - drug effects</subject><subject>Cupriavidus necator - drug effects</subject><subject>Cupriavidus necator - growth & development</subject><subject>Cupriavidus necator - metabolism</subject><subject>Hydrolysis</subject><subject>Hydroxybutyrates - chemistry</subject><subject>Hydroxybutyrates - metabolism</subject><subject>Poly-3-hydroxybutyrate</subject><subject>Polyesters - chemistry</subject><subject>Polyesters - metabolism</subject><subject>Rice paddy straw</subject><subject>Spectrum Analysis, Raman</subject><subject>Thermogravimetry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v1DAQxS0EokvhK1Q-lkOC_23s3IAVUKRKIARny7HHrLdJvNhORTjw2XHZliunkUbvvZn3Q-iCkpYS2r06tOEwhDgZ2zJCty2RLeHiEdpQJfuGEMIfow2hgjaKcnKGnuV8qNtuS9VTdMY6Rjop6Ab93u1NMrZACr9MCXHG0eNjHNeGN_vVpfhzHZayJlMAX36-evsSH1N0iwWHfYoT_mLGXOIcDIalpHjcG7zkMH_HZsZmvDFjaI4JSoIa4PDfj3PGHsBVm715jp74mgAv7uc5-vb-3dfdVXP96cPH3ZvrxgouSiMNl6IflPW-54wJ5RhRTjBvnOw4GXrnGBir_MBqLbkVxHnZDcINnG07P_BzdHnKrd__WCAXPYVsYRzNDHHJmkqiVK8I66q0O0ltijkn8PqYwmTSqinRd-z1QT-w13fsNZG6sq_Gi_sbyzCB-2d7gF0Fr08CqE1vAySdbYC5sgwJbNEuhv_d-APBE5tB</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Saratale, Ganesh D.</creator><creator>Oh, Min-Kyu</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150901</creationdate><title>Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock</title><author>Saratale, Ganesh D. ; Oh, Min-Kyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-7a3749b8cff932248d208d42fad7630b9dd2eac8fb26747540df76b4db3256fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Alkalies - pharmacology</topic><topic>Alkaline pretreatment</topic><topic>Biomass</topic><topic>Carbohydrate Metabolism - drug effects</topic><topic>Cupriavidus necator - drug effects</topic><topic>Cupriavidus necator - growth & development</topic><topic>Cupriavidus necator - metabolism</topic><topic>Hydrolysis</topic><topic>Hydroxybutyrates - chemistry</topic><topic>Hydroxybutyrates - metabolism</topic><topic>Poly-3-hydroxybutyrate</topic><topic>Polyesters - chemistry</topic><topic>Polyesters - metabolism</topic><topic>Rice paddy straw</topic><topic>Spectrum Analysis, Raman</topic><topic>Thermogravimetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saratale, Ganesh D.</creatorcontrib><creatorcontrib>Oh, Min-Kyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saratale, Ganesh D.</au><au>Oh, Min-Kyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>80</volume><spage>627</spage><epage>635</epage><pages>627-635</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Alkaline pretreatment using NaOH, KOH, or NaOCl has been applied to various types of waste biomass to enhance enzymatic digestibility. Pretreatment (2% NaOH, 121°C, 30min) of rice paddy straw (PS) resulted in a maximum yield of 703mg of reducing sugar per gram of PS with 84.19% hydrolysis yield after a two-step enzymatic hydrolysis process. Ralstonia eutropha ATCC 17699 was tested for its ability to synthesize poly-3-hydroxybutyrate (PHB) using PS hydrolysates as its sole carbon source. It is noteworthy that dry cell weight, polyhydroxyalkanoate (PHA) accumulation and PHB yield with the use of laboratory-grade sugars were similar to those achieved with PS-derived sugars. Under optimized conditions, we observed maximal PHA accumulation (75.45%) and PHB production (11.42g/L) within 48h of fermentation. After PHB recovery, the physicochemical properties of PHB were determined by various analytical techniques, showed the results were consistent with the characteristics of a standard polymer of PHB. 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkalies - pharmacology Alkaline pretreatment Biomass Carbohydrate Metabolism - drug effects Cupriavidus necator - drug effects Cupriavidus necator - growth & development Cupriavidus necator - metabolism Hydrolysis Hydroxybutyrates - chemistry Hydroxybutyrates - metabolism Poly-3-hydroxybutyrate Polyesters - chemistry Polyesters - metabolism Rice paddy straw Spectrum Analysis, Raman Thermogravimetry |
| title | Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock |
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