First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex
[Display omitted] •Parasites were isolated from a new focus of human cutaneous leishmaniasis.•DNA sequence analysis showed these to be a new species of Leishmania.•The parasites belong to the Leishmania enriettii complex. An active case detection approach with PCR diagnosis was used in the Ho Distri...
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| Veröffentlicht in: | International journal for parasitology 2015-09, Vol.45 (11), p.679-684 |
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| container_title | International journal for parasitology |
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| creator | Kwakye-Nuako, Godwin Mosore, Mba-Tihssommah Duplessis, Christopher Bates, Michelle D. Puplampu, Naiki Mensah-Attipoe, Israel Desewu, Kwame Afegbe, Godwin Asmah, Richard H. Jamjoom, Manal B. Ayeh-Kumi, Patrick F. Boakye, Daniel A. Bates, Paul A. |
| description | [Display omitted]
•Parasites were isolated from a new focus of human cutaneous leishmaniasis.•DNA sequence analysis showed these to be a new species of Leishmania.•The parasites belong to the Leishmania enriettii complex.
An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens. |
| doi_str_mv | 10.1016/j.ijpara.2015.05.001 |
| format | Article |
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•Parasites were isolated from a new focus of human cutaneous leishmaniasis.•DNA sequence analysis showed these to be a new species of Leishmania.•The parasites belong to the Leishmania enriettii complex.
An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/j.ijpara.2015.05.001</identifier><identifier>PMID: 26099650</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Cluster Analysis ; Cutaneous leishmaniasis ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; DNA, Ribosomal Spacer - chemistry ; DNA, Ribosomal Spacer - genetics ; Female ; Ghana ; Humans ; Leishmania ; Leishmania enriettii - classification ; Leishmania enriettii - genetics ; Leishmania enriettii - isolation & purification ; Leishmaniasis, Cutaneous - diagnosis ; Leishmaniasis, Cutaneous - parasitology ; Male ; Middle Aged ; Phylogeny ; Polymerase Chain Reaction ; Ribosomal Proteins - genetics ; RNA Polymerase II - genetics ; Sequence Analysis, DNA ; Young Adult</subject><ispartof>International journal for parasitology, 2015-09, Vol.45 (11), p.679-684</ispartof><rights>2015 The Authors</rights><rights>Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-ca41c6c641185399f3991480a76bb70af73a2d9465fdcde885b4221dcfa6a15c3</citedby><cites>FETCH-LOGICAL-c478t-ca41c6c641185399f3991480a76bb70af73a2d9465fdcde885b4221dcfa6a15c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpara.2015.05.001$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26099650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kwakye-Nuako, Godwin</creatorcontrib><creatorcontrib>Mosore, Mba-Tihssommah</creatorcontrib><creatorcontrib>Duplessis, Christopher</creatorcontrib><creatorcontrib>Bates, Michelle D.</creatorcontrib><creatorcontrib>Puplampu, Naiki</creatorcontrib><creatorcontrib>Mensah-Attipoe, Israel</creatorcontrib><creatorcontrib>Desewu, Kwame</creatorcontrib><creatorcontrib>Afegbe, Godwin</creatorcontrib><creatorcontrib>Asmah, Richard H.</creatorcontrib><creatorcontrib>Jamjoom, Manal B.</creatorcontrib><creatorcontrib>Ayeh-Kumi, Patrick F.</creatorcontrib><creatorcontrib>Boakye, Daniel A.</creatorcontrib><creatorcontrib>Bates, Paul A.</creatorcontrib><title>First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>[Display omitted]
•Parasites were isolated from a new focus of human cutaneous leishmaniasis.•DNA sequence analysis showed these to be a new species of Leishmania.•The parasites belong to the Leishmania enriettii complex.
An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cluster Analysis</subject><subject>Cutaneous leishmaniasis</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Ribosomal Spacer - chemistry</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Female</subject><subject>Ghana</subject><subject>Humans</subject><subject>Leishmania</subject><subject>Leishmania enriettii - classification</subject><subject>Leishmania enriettii - genetics</subject><subject>Leishmania enriettii - isolation & purification</subject><subject>Leishmaniasis, Cutaneous - diagnosis</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Ribosomal Proteins - genetics</subject><subject>RNA Polymerase II - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Young Adult</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2L3DAMhk1p6U6n_Qel-NhLpnbifF0KZdndLgz00p6N4iiMhsROraQf_6U_th6yXXpakDBIj_UivUK81eqgla4-nA90niHCIVe6PKgUSj8TO93UbaZ0UT4XO6VyldWlbq_EK-ZzAsrCmJfiKq9U21al2ok_txR5kcRhhIWCl2GQID3-lDyjI-RL4YjEpwk8gYzIc_BM3YhyCFGe1lSXbl3AY1hZjo8oE0vy8u4EHiT4XroRmGkgtwml3nLC_2ejj4TLQiRdmOYRf70WLwYYGd88vHvx7fbm6_Xn7Pjl7v760zFzpm6WzIHRrnKV0bopi7YdUmrTKKirrqsVDHUBed-aqhx612PTlJ3Jc927ASrQpSv24v02d47h-4q82InY4ThuS1ldq6Zp61wVCTUb6mJgjjjYOdIE8bfVyl58sWe7-WIvvliVIrmxF-8eFNZuwv7x0z8jEvBxAzDt-YMwWk7X9w57iugW2wd6WuEvww-j8g</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Kwakye-Nuako, Godwin</creator><creator>Mosore, Mba-Tihssommah</creator><creator>Duplessis, Christopher</creator><creator>Bates, Michelle D.</creator><creator>Puplampu, Naiki</creator><creator>Mensah-Attipoe, Israel</creator><creator>Desewu, Kwame</creator><creator>Afegbe, Godwin</creator><creator>Asmah, Richard H.</creator><creator>Jamjoom, Manal B.</creator><creator>Ayeh-Kumi, Patrick F.</creator><creator>Boakye, Daniel A.</creator><creator>Bates, Paul A.</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150901</creationdate><title>First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex</title><author>Kwakye-Nuako, Godwin ; Mosore, Mba-Tihssommah ; Duplessis, Christopher ; Bates, Michelle D. ; Puplampu, Naiki ; Mensah-Attipoe, Israel ; Desewu, Kwame ; Afegbe, Godwin ; Asmah, Richard H. ; Jamjoom, Manal B. ; Ayeh-Kumi, Patrick F. ; Boakye, Daniel A. ; Bates, Paul A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-ca41c6c641185399f3991480a76bb70af73a2d9465fdcde885b4221dcfa6a15c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cluster Analysis</topic><topic>Cutaneous leishmaniasis</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Ribosomal Spacer - chemistry</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Female</topic><topic>Ghana</topic><topic>Humans</topic><topic>Leishmania</topic><topic>Leishmania enriettii - classification</topic><topic>Leishmania enriettii - genetics</topic><topic>Leishmania enriettii - isolation & purification</topic><topic>Leishmaniasis, Cutaneous - diagnosis</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Ribosomal Proteins - genetics</topic><topic>RNA Polymerase II - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwakye-Nuako, Godwin</creatorcontrib><creatorcontrib>Mosore, Mba-Tihssommah</creatorcontrib><creatorcontrib>Duplessis, Christopher</creatorcontrib><creatorcontrib>Bates, Michelle D.</creatorcontrib><creatorcontrib>Puplampu, Naiki</creatorcontrib><creatorcontrib>Mensah-Attipoe, Israel</creatorcontrib><creatorcontrib>Desewu, Kwame</creatorcontrib><creatorcontrib>Afegbe, Godwin</creatorcontrib><creatorcontrib>Asmah, Richard H.</creatorcontrib><creatorcontrib>Jamjoom, Manal B.</creatorcontrib><creatorcontrib>Ayeh-Kumi, Patrick F.</creatorcontrib><creatorcontrib>Boakye, Daniel A.</creatorcontrib><creatorcontrib>Bates, Paul A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwakye-Nuako, Godwin</au><au>Mosore, Mba-Tihssommah</au><au>Duplessis, Christopher</au><au>Bates, Michelle D.</au><au>Puplampu, Naiki</au><au>Mensah-Attipoe, Israel</au><au>Desewu, Kwame</au><au>Afegbe, Godwin</au><au>Asmah, Richard H.</au><au>Jamjoom, Manal B.</au><au>Ayeh-Kumi, Patrick F.</au><au>Boakye, Daniel A.</au><au>Bates, Paul A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>45</volume><issue>11</issue><spage>679</spage><epage>684</epage><pages>679-684</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><abstract>[Display omitted]
•Parasites were isolated from a new focus of human cutaneous leishmaniasis.•DNA sequence analysis showed these to be a new species of Leishmania.•The parasites belong to the Leishmania enriettii complex.
An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26099650</pmid><doi>10.1016/j.ijpara.2015.05.001</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Aged Child Child, Preschool Cluster Analysis Cutaneous leishmaniasis DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - genetics Female Ghana Humans Leishmania Leishmania enriettii - classification Leishmania enriettii - genetics Leishmania enriettii - isolation & purification Leishmaniasis, Cutaneous - diagnosis Leishmaniasis, Cutaneous - parasitology Male Middle Aged Phylogeny Polymerase Chain Reaction Ribosomal Proteins - genetics RNA Polymerase II - genetics Sequence Analysis, DNA Young Adult |
| title | First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex |
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