Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media

The rate of p-nitrophenyl palmitate (pNPP) hydrolysis in n-heptane by a lipase preparation from Pseudomonas cepacia was studied. In organic media, the reaction rate could be easily measured by spectrophotometry after extraction of the liberated p-nitrophenol by an alkaline water phase. Optimal condi...

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Veröffentlicht in:	Enzyme and microbial technology 1996-05, Vol.18 (6), p.417-422
Hauptverfasser:	Pencreac'h, Gaëlle, Baratti, Jacques C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Lipase lipase assay Methods. Procedures. Technologies nonconventional media organic media Pseudomonas cepacia
Online-Zugang:	Volltext
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