Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media
The rate of p-nitrophenyl palmitate (pNPP) hydrolysis in n-heptane by a lipase preparation from Pseudomonas cepacia was studied. In organic media, the reaction rate could be easily measured by spectrophotometry after extraction of the liberated p-nitrophenol by an alkaline water phase. Optimal condi...
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| Veröffentlicht in: | Enzyme and microbial technology 1996-05, Vol.18 (6), p.417-422 |
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| container_title | Enzyme and microbial technology |
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| creator | Pencreac'h, Gaëlle Baratti, Jacques C. |
| description | The rate of
p-nitrophenyl palmitate (pNPP) hydrolysis in
n-heptane by a lipase preparation from
Pseudomonas cepacia was studied. In organic media, the reaction rate could be easily measured by spectrophotometry after extraction of the liberated
p-nitrophenol by an alkaline water phase. Optimal conditions for substrate hydrolysis were selected and proposed for the easy and fast assay of lipase activity in organic media. Agitation and the amount of added water showed little effect on reaction rates. A temperature of 37°C and substrate concentration of 50 m
m were selected. The reaction rate in
n-heptane was only 0.16% of that in water. |
| doi_str_mv | 10.1016/0141-0229(95)00120-4 |
| format | Article |
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p-nitrophenyl palmitate (pNPP) hydrolysis in
n-heptane by a lipase preparation from
Pseudomonas cepacia was studied. In organic media, the reaction rate could be easily measured by spectrophotometry after extraction of the liberated
p-nitrophenol by an alkaline water phase. Optimal conditions for substrate hydrolysis were selected and proposed for the easy and fast assay of lipase activity in organic media. Agitation and the amount of added water showed little effect on reaction rates. A temperature of 37°C and substrate concentration of 50 m
m were selected. The reaction rate in
n-heptane was only 0.16% of that in water.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/0141-0229(95)00120-4</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Lipase ; lipase assay ; Methods. Procedures. Technologies ; nonconventional media ; organic media ; Pseudomonas cepacia</subject><ispartof>Enzyme and microbial technology, 1996-05, Vol.18 (6), p.417-422</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-13c9d803f4c3671aae31d83daee84b3a222d11ddef90181038d3fdd4bba550f93</citedby><cites>FETCH-LOGICAL-c497t-13c9d803f4c3671aae31d83daee84b3a222d11ddef90181038d3fdd4bba550f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0141022995001204$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3049761$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Pencreac'h, Gaëlle</creatorcontrib><creatorcontrib>Baratti, Jacques C.</creatorcontrib><title>Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media</title><title>Enzyme and microbial technology</title><description>The rate of
p-nitrophenyl palmitate (pNPP) hydrolysis in
n-heptane by a lipase preparation from
Pseudomonas cepacia was studied. In organic media, the reaction rate could be easily measured by spectrophotometry after extraction of the liberated
p-nitrophenol by an alkaline water phase. Optimal conditions for substrate hydrolysis were selected and proposed for the easy and fast assay of lipase activity in organic media. Agitation and the amount of added water showed little effect on reaction rates. A temperature of 37°C and substrate concentration of 50 m
m were selected. The reaction rate in
n-heptane was only 0.16% of that in water.</description><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipase</subject><subject>lipase assay</subject><subject>Methods. Procedures. Technologies</subject><subject>nonconventional media</subject><subject>organic media</subject><subject>Pseudomonas cepacia</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhSMEUpfCP-jBB4TgELBjZxNzQKoqaJEq0UM5W7P2mB2U2MH2VsqP6X8l6VY9ltNcvjdv5r2qOhP8k-Bi-5kLJWreNPqDbj9yLhpeqxfVRvSdrrnm-mW1eUJOqtc5_-ELpRTfVPdXs0txmDNlFj2b6kAlxWmPYR7YBMNIBQoyCizUe5wKBGS7mZU9spuMBxfHGCAzixNYAjbQBBm_sHOWaZwGZAVzYT6mB4XDgmmkAIViWO2OOANb6I7KvNrE9BsCWTaiI3hTvfIwZHz7OE-rX9-_3V5c1dc_L39cnF_XVumu1EJa7XouvbJy2wkAlML10gFir3YSmqZxQjiHXnPRCy57J71zareDtuVey9Pq_XHvlOLfw3KyGSlbHIbl3XjIRnS8V0q3_wfbTmxVpxZQHUGbYs4JvZkSjZBmI7hZSzNrI2ZtxOjWPJRmVtm7x_2QLQw-QbCUn7SSL_9uxYJ9PWK4hHJHmEy2hMEumSW0xbhIz_v8AyZCrbI</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Pencreac'h, Gaëlle</creator><creator>Baratti, Jacques C.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7QL</scope><scope>7T7</scope><scope>C1K</scope></search><sort><creationdate>19960501</creationdate><title>Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media</title><author>Pencreac'h, Gaëlle ; Baratti, Jacques C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-13c9d803f4c3671aae31d83daee84b3a222d11ddef90181038d3fdd4bba550f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lipase</topic><topic>lipase assay</topic><topic>Methods. Procedures. Technologies</topic><topic>nonconventional media</topic><topic>organic media</topic><topic>Pseudomonas cepacia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pencreac'h, Gaëlle</creatorcontrib><creatorcontrib>Baratti, Jacques C.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pencreac'h, Gaëlle</au><au>Baratti, Jacques C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media</atitle><jtitle>Enzyme and microbial technology</jtitle><date>1996-05-01</date><risdate>1996</risdate><volume>18</volume><issue>6</issue><spage>417</spage><epage>422</epage><pages>417-422</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>The rate of
p-nitrophenyl palmitate (pNPP) hydrolysis in
n-heptane by a lipase preparation from
Pseudomonas cepacia was studied. In organic media, the reaction rate could be easily measured by spectrophotometry after extraction of the liberated
p-nitrophenol by an alkaline water phase. Optimal conditions for substrate hydrolysis were selected and proposed for the easy and fast assay of lipase activity in organic media. Agitation and the amount of added water showed little effect on reaction rates. A temperature of 37°C and substrate concentration of 50 m
m were selected. The reaction rate in
n-heptane was only 0.16% of that in water.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/0141-0229(95)00120-4</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 1996-05, Vol.18 (6), p.417-422 |
| issn | 0141-0229 1879-0909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17084495 |
| source | Elsevier ScienceDirect Journals |
| subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Lipase lipase assay Methods. Procedures. Technologies nonconventional media organic media Pseudomonas cepacia |
| title | Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media |
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