SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae
The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the e...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 1996-03, Vol.142 (3), p.477-484 |
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| container_end_page | 484 |
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| container_issue | 3 |
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| container_title | Microbiology (Society for General Microbiology) |
| container_volume | 142 |
| creator | Cliften, P Wang, Y Mochizuki, D Miyakawa, T Wangspa, R Hughes, J Yakemoto, J.Y |
| description | The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin. |
| doi_str_mv | 10.1099/13500872-142-3-477 |
| format | Article |
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A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/13500872-142-3-477</identifier><identifier>PMID: 8868422</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Anti-Bacterial Agents - pharmacology ; antifungal properties ; Bacterial Proteins - pharmacology ; Base Sequence ; Biological and medical sciences ; Cell Division - drug effects ; Cloning, Molecular ; Drug Resistance, Microbial - genetics ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - genetics ; genbank/scu10427 ; Genes, Fungal ; Genes. Genome ; Mixed Function Oxygenases ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutation ; phytotoxins ; Pseudomonas - metabolism ; Pseudomonas syringae ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae Proteins ; Sequence Analysis</subject><ispartof>Microbiology (Society for General Microbiology), 1996-03, Vol.142 (3), p.477-484</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-d9d6425c24ab7b7b6ad5d107c48dc11fc7da2221c530355d213ae3352d5a297c3</citedby><cites>FETCH-LOGICAL-c525t-d9d6425c24ab7b7b6ad5d107c48dc11fc7da2221c530355d213ae3352d5a297c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3009345$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8868422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cliften, P</creatorcontrib><creatorcontrib>Wang, Y</creatorcontrib><creatorcontrib>Mochizuki, D</creatorcontrib><creatorcontrib>Miyakawa, T</creatorcontrib><creatorcontrib>Wangspa, R</creatorcontrib><creatorcontrib>Hughes, J</creatorcontrib><creatorcontrib>Yakemoto, J.Y</creatorcontrib><title>SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology (Reading)</addtitle><description>The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. 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Genome</subject><subject>Mixed Function Oxygenases</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>phytotoxins</subject><subject>Pseudomonas - metabolism</subject><subject>Pseudomonas syringae</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Analysis</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkF1rFDEUhoMotbb-AUHMhYgXjp6cTCYzl1JsLRQK3e6FV-FskpmJ7ExqsmvZf2-WXUvJRQLvcz7yMPZOwFcBXfdNSAXQaqxEjZWsaq1fsFNRN6pCaOFleReg2hOv2ZucfwOUEMQJO2nbpq0RT9ly8esOv3Dig589n731OVPa8T4mnncpzEOcdjbMfEjxcTPyMI9hFTYhzjz2fEHWjpT2iM_c-uT_hhzIn7NXPa2zf3u8z9jy8sf9xc_q5vbq-uL7TWUVqk3lOtfUqCzWtNLlNOSUE6Bt3TorRG-1I0QUVkmQSjkUkryUCp0i7LSVZ-zToe9Din-2Pm_MFLL16zXNPm6zERpa0aqugHgAbYo5J9-bhxSm8lEjwOxdmv8uTXFppCkuS9H7Y_ftavLuqeQor-QfjzllS-s-0WxDfsIkQCdrVbDPB2wMw_gYkjfF9RTKJqsQy8L2-cQPB7SnaGhIpdtygSAkCCVRNyD_AS7vkrI</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Cliften, P</creator><creator>Wang, Y</creator><creator>Mochizuki, D</creator><creator>Miyakawa, T</creator><creator>Wangspa, R</creator><creator>Hughes, J</creator><creator>Yakemoto, J.Y</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19960301</creationdate><title>SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae</title><author>Cliften, P ; Wang, Y ; Mochizuki, D ; Miyakawa, T ; Wangspa, R ; Hughes, J ; Yakemoto, J.Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-d9d6425c24ab7b7b6ad5d107c48dc11fc7da2221c530355d213ae3352d5a297c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antifungal properties</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cloning, Molecular</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Fundamental and applied biological sciences. 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| ispartof | Microbiology (Society for General Microbiology), 1996-03, Vol.142 (3), p.477-484 |
| issn | 1350-0872 1465-2080 |
| language | eng |
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| subjects | Amino Acid Sequence Anti-Bacterial Agents - pharmacology antifungal properties Bacterial Proteins - pharmacology Base Sequence Biological and medical sciences Cell Division - drug effects Cloning, Molecular Drug Resistance, Microbial - genetics Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics genbank/scu10427 Genes, Fungal Genes. Genome Mixed Function Oxygenases Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation phytotoxins Pseudomonas - metabolism Pseudomonas syringae Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae Proteins Sequence Analysis |
| title | SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae |
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