The effect of up-regulated expression of Rap1GAP on the invasion ability of HL-60 cells in vitro and in vivo
To investigate the effect of up-regulation of Rap1GAP on the invasion ability of leukemic HL-60 cells in vitro, and to establish leukemia mouse model to verify the effects in vivo. Quantitative RT-PCR and Western blot methods were used to detect the expression of Rap1GAP in Venus/HL-60 (vehicle cont...
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| Veröffentlicht in: | Zhōnghuá xuèyèxué zázhì 2015-07, Vol.36 (7), p.570-574 |
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| container_title | Zhōnghuá xuèyèxué zázhì |
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| creator | Qiu, Tingting Li, Depeng Li, Zhenyu Xu, Kailin Qi, Xiaofei Cen, Jiannong Chen, Zixing |
| description | To investigate the effect of up-regulation of Rap1GAP on the invasion ability of leukemic HL-60 cells in vitro, and to establish leukemia mouse model to verify the effects in vivo.
Quantitative RT-PCR and Western blot methods were used to detect the expression of Rap1GAP in Venus/HL-60 (vehicle control) and Rap1GAP/HL-60 cells (R1 andR2). Transwell method was used to examine the invasion ability in vitro. Quantitative RT-PCR and gelatin zymograph were used to study the expression of MMP-2 and MMP-9. Four-week-old BALB/c nu/nu mice were pre-treated and inoculated with leukemic cells from different groups, several index including survival time were then monitored.
Rap1GAP mRNA level of R1 and R2 increased about 16-17 folds as compared to the control cells. The invasion rate of R1 and R2 are (55 ± 5)% and (59 ± 4)%, which are significantly higher than (14 ± 4)% of the control cells. The mRNA level of MMP-9 was up-regulated about 12.0 folds in R1 and R2 cells compared to the corresponding control cells. The media |
| doi_str_mv | 10.3760/cma.j.issn.0253-2727.2015.07.009 |
| format | Article |
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Quantitative RT-PCR and Western blot methods were used to detect the expression of Rap1GAP in Venus/HL-60 (vehicle control) and Rap1GAP/HL-60 cells (R1 andR2). Transwell method was used to examine the invasion ability in vitro. Quantitative RT-PCR and gelatin zymograph were used to study the expression of MMP-2 and MMP-9. Four-week-old BALB/c nu/nu mice were pre-treated and inoculated with leukemic cells from different groups, several index including survival time were then monitored.
Rap1GAP mRNA level of R1 and R2 increased about 16-17 folds as compared to the control cells. The invasion rate of R1 and R2 are (55 ± 5)% and (59 ± 4)%, which are significantly higher than (14 ± 4)% of the control cells. The mRNA level of MMP-9 was up-regulated about 12.0 folds in R1 and R2 cells compared to the corresponding control cells. The media</description><identifier>ISSN: 0253-2727</identifier><identifier>DOI: 10.3760/cma.j.issn.0253-2727.2015.07.009</identifier><identifier>PMID: 26304080</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; GTPase-Activating Proteins - metabolism ; HL-60 Cells ; Humans ; Leukemia ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 9 - metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Invasiveness ; RNA, Messenger ; Transcriptional Activation ; Up-Regulation</subject><ispartof>Zhōnghuá xuèyèxué zázhì, 2015-07, Vol.36 (7), p.570-574</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26304080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Tingting</creatorcontrib><creatorcontrib>Li, Depeng</creatorcontrib><creatorcontrib>Li, Zhenyu</creatorcontrib><creatorcontrib>Xu, Kailin</creatorcontrib><creatorcontrib>Qi, Xiaofei</creatorcontrib><creatorcontrib>Cen, Jiannong</creatorcontrib><creatorcontrib>Chen, Zixing</creatorcontrib><title>The effect of up-regulated expression of Rap1GAP on the invasion ability of HL-60 cells in vitro and in vivo</title><title>Zhōnghuá xuèyèxué zázhì</title><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><description>To investigate the effect of up-regulation of Rap1GAP on the invasion ability of leukemic HL-60 cells in vitro, and to establish leukemia mouse model to verify the effects in vivo.
Quantitative RT-PCR and Western blot methods were used to detect the expression of Rap1GAP in Venus/HL-60 (vehicle control) and Rap1GAP/HL-60 cells (R1 andR2). Transwell method was used to examine the invasion ability in vitro. Quantitative RT-PCR and gelatin zymograph were used to study the expression of MMP-2 and MMP-9. Four-week-old BALB/c nu/nu mice were pre-treated and inoculated with leukemic cells from different groups, several index including survival time were then monitored.
Rap1GAP mRNA level of R1 and R2 increased about 16-17 folds as compared to the control cells. The invasion rate of R1 and R2 are (55 ± 5)% and (59 ± 4)%, which are significantly higher than (14 ± 4)% of the control cells. The mRNA level of MMP-9 was up-regulated about 12.0 folds in R1 and R2 cells compared to the corresponding control cells. The media</description><subject>Animals</subject><subject>GTPase-Activating Proteins - metabolism</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Neoplasm Invasiveness</subject><subject>RNA, Messenger</subject><subject>Transcriptional Activation</subject><subject>Up-Regulation</subject><issn>0253-2727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLw1AQhe9CsaX2L8hddpM495l0WYq2QkGRug6TZKJX8jI3Kfbfm9rqajhzvjMchrGFgFBFFu6zCsPP0HlfhyCNCmQko1CCMCFEIcDyik3_9xM2996lYISysVJwwybSKtAQw5SV-w_iVBSU9bwp-NAGHb0PJfaUc_puOxqjTX2yXrEVm9ULH1U_Zlx9wF8LU1e6_nhCtrvAAs-oLP3o84Pru4ZjnZ_Fobll1wWWnuaXOWNvjw_79TbYPW-e1qtd0App-4CUkYosGq1IUK7lMrXaEFEs01yhXhrSAHoZYYoyRpmhVRqEKoQsJFhQM7Y432275msg3yeV86daWFMz-EREEBkTWyFG9O6CDmlFedJ2rsLumPx9SP0Ar0dqFw</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Qiu, Tingting</creator><creator>Li, Depeng</creator><creator>Li, Zhenyu</creator><creator>Xu, Kailin</creator><creator>Qi, Xiaofei</creator><creator>Cen, Jiannong</creator><creator>Chen, Zixing</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>The effect of up-regulated expression of Rap1GAP on the invasion ability of HL-60 cells in vitro and in vivo</title><author>Qiu, Tingting ; Li, Depeng ; Li, Zhenyu ; Xu, Kailin ; Qi, Xiaofei ; Cen, Jiannong ; Chen, Zixing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-e3523e6a543e1ed429b645eee82bd3a495e400497aba28a2ca634013f12f20603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2015</creationdate><topic>Animals</topic><topic>GTPase-Activating Proteins - metabolism</topic><topic>HL-60 Cells</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Neoplasm Invasiveness</topic><topic>RNA, Messenger</topic><topic>Transcriptional Activation</topic><topic>Up-Regulation</topic><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Tingting</creatorcontrib><creatorcontrib>Li, Depeng</creatorcontrib><creatorcontrib>Li, Zhenyu</creatorcontrib><creatorcontrib>Xu, Kailin</creatorcontrib><creatorcontrib>Qi, Xiaofei</creatorcontrib><creatorcontrib>Cen, Jiannong</creatorcontrib><creatorcontrib>Chen, Zixing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Tingting</au><au>Li, Depeng</au><au>Li, Zhenyu</au><au>Xu, Kailin</au><au>Qi, Xiaofei</au><au>Cen, Jiannong</au><au>Chen, Zixing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of up-regulated expression of Rap1GAP on the invasion ability of HL-60 cells in vitro and in vivo</atitle><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><date>2015-07</date><risdate>2015</risdate><volume>36</volume><issue>7</issue><spage>570</spage><epage>574</epage><pages>570-574</pages><issn>0253-2727</issn><abstract>To investigate the effect of up-regulation of Rap1GAP on the invasion ability of leukemic HL-60 cells in vitro, and to establish leukemia mouse model to verify the effects in vivo.
Quantitative RT-PCR and Western blot methods were used to detect the expression of Rap1GAP in Venus/HL-60 (vehicle control) and Rap1GAP/HL-60 cells (R1 andR2). Transwell method was used to examine the invasion ability in vitro. Quantitative RT-PCR and gelatin zymograph were used to study the expression of MMP-2 and MMP-9. Four-week-old BALB/c nu/nu mice were pre-treated and inoculated with leukemic cells from different groups, several index including survival time were then monitored.
Rap1GAP mRNA level of R1 and R2 increased about 16-17 folds as compared to the control cells. The invasion rate of R1 and R2 are (55 ± 5)% and (59 ± 4)%, which are significantly higher than (14 ± 4)% of the control cells. The mRNA level of MMP-9 was up-regulated about 12.0 folds in R1 and R2 cells compared to the corresponding control cells. The media</abstract><cop>China</cop><pmid>26304080</pmid><doi>10.3760/cma.j.issn.0253-2727.2015.07.009</doi><tpages>5</tpages></addata></record> |
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| ispartof | Zhōnghuá xuèyèxué zázhì, 2015-07, Vol.36 (7), p.570-574 |
| issn | 0253-2727 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_1707558611 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals GTPase-Activating Proteins - metabolism HL-60 Cells Humans Leukemia Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - metabolism Mice Mice, Inbred BALB C Mice, Nude Neoplasm Invasiveness RNA, Messenger Transcriptional Activation Up-Regulation |
| title | The effect of up-regulated expression of Rap1GAP on the invasion ability of HL-60 cells in vitro and in vivo |
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