Sensitivity of Streptococcus thermophilus to chemical permeabilization
Streptococcus thermophilus cultures were treated with conjugated and unconjugated bile salts and tested for beta-galactosidase activity. Na-deoxycholate and chenodeoxycholate were more efficient permeabilizing agents than cholate, and all three bile salts were superior to their corresponding glyco-...
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| Veröffentlicht in: | Current microbiology 1996-02, Vol.32 (2), p.101-105 |
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| container_end_page | 105 |
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| container_issue | 2 |
| container_start_page | 101 |
| container_title | Current microbiology |
| container_volume | 32 |
| creator | Somkuti, G.A. (USDA, ARS, Eastern Regional Research Center, Philadelphia.) Dominiecki, M.E Steinberg, D.H |
| description | Streptococcus thermophilus cultures were treated with conjugated and unconjugated bile salts and tested for beta-galactosidase activity. Na-deoxycholate and chenodeoxycholate were more efficient permeabilizing agents than cholate, and all three bile salts were superior to their corresponding glyco- and tauro-conjugates. Treatment with sodium dodecyl sulfate resulted in the highest measurable beta-galactosidase levels in permeabilized cells, whereas response to Triton X-100 was variable and strain dependent. Na-deoxycholate, chenodeoxycholate, and sodium dodecyl sulfate caused cell injury and arrested culture growth for 4 h or longer. The nongrowing permeabilized biomass of S. thermophilus was used to hydrolyze lactose in aqueous solutions and milk |
| doi_str_mv | 10.1007/s002849900018 |
| format | Article |
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The nongrowing permeabilized biomass of S. thermophilus was used to hydrolyze lactose in aqueous solutions and milk</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002849900018</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Action of physical and chemical agents on bacteria ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Bacteriology ; BETA GALACTOSIDASA ; BETA GALACTOSIDASE ; Biological and medical sciences ; BIOMASA ; BIOMASSE ; Biotechnology ; Fundamental and applied biological sciences. 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Psychology</topic><topic>HIDROLISIS</topic><topic>HYDROLYSE</topic><topic>LACTOSA</topic><topic>LACTOSE</topic><topic>Microbiology</topic><topic>SALES BILIARES</topic><topic>SEL BILIAIRE</topic><topic>STREPTOCOCCUS THERMOPHILUS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Somkuti, G.A. 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(USDA, ARS, Eastern Regional Research Center, Philadelphia.)</au><au>Dominiecki, M.E</au><au>Steinberg, D.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitivity of Streptococcus thermophilus to chemical permeabilization</atitle><jtitle>Current microbiology</jtitle><date>1996-02-01</date><risdate>1996</risdate><volume>32</volume><issue>2</issue><spage>101</spage><epage>105</epage><pages>101-105</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>Streptococcus thermophilus cultures were treated with conjugated and unconjugated bile salts and tested for beta-galactosidase activity. Na-deoxycholate and chenodeoxycholate were more efficient permeabilizing agents than cholate, and all three bile salts were superior to their corresponding glyco- and tauro-conjugates. 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| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 1996-02, Vol.32 (2), p.101-105 |
| issn | 0343-8651 1432-0991 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Action of physical and chemical agents on bacteria ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Bacteriology BETA GALACTOSIDASA BETA GALACTOSIDASE Biological and medical sciences BIOMASA BIOMASSE Biotechnology Fundamental and applied biological sciences. Psychology HIDROLISIS HYDROLYSE LACTOSA LACTOSE Microbiology SALES BILIARES SEL BILIAIRE STREPTOCOCCUS THERMOPHILUS |
| title | Sensitivity of Streptococcus thermophilus to chemical permeabilization |
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