Testicular immunohistochemical and Ultrastructural changes associated with chronic cholestasis in rats: Effect of ursodeoxycholic acid
Testicular atrophy has been commonly reported in patients with chronic liver diseases. Ursodeoxycholic acid is the most widely used drug for the treatment of many liver diseases. However, its effect on testicular ultrastructure associated with chronic cholestasis has never been studied. Thus, this s...
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| Veröffentlicht in: | Life sciences (1973) 2015-09, Vol.136, p.52-59 |
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| description | Testicular atrophy has been commonly reported in patients with chronic liver diseases. Ursodeoxycholic acid is the most widely used drug for the treatment of many liver diseases. However, its effect on testicular ultrastructure associated with chronic cholestasis has never been studied. Thus, this study aimed to assess how chronic obstructive jaundice affects the testicular ultrastructure and whether it affects the androgen receptor or the proliferating cell nuclear antigen. The role of ursodeoxycholic acid was also investigated.
Cholestasis was induced by bile duct ligation. Samples were collected 4weeks postoperative. Testicular changes were assessed using immunohistochemistry and transmission electron microscopy.
Chronic cholestasis resulted in testicular atrophy evidenced by shrinkage and deformation of seminiferous tubules, thickening of peritubular boundaries, vacuolation, disorganization of germ cells, and maturation arrest. This was accompanied by decreased immunoreactivity of androgen receptors and proliferating cell nuclear antigen. Administration of ursodeoxycholic acid improved the testicular morphology and reversed cholestasis-induced immunohistochemical and ultrastructural changes.
Ursodeoxycholic acid can improve the testicular ultrastructure and restore the spermatogenic process in rats with chronic cholestasis. These findings support the clinical application of ursodeoxycholic acid in cholestatic patients especially those with hypogonadism. |
| doi_str_mv | 10.1016/j.lfs.2015.05.027 |
| format | Article |
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Cholestasis was induced by bile duct ligation. Samples were collected 4weeks postoperative. Testicular changes were assessed using immunohistochemistry and transmission electron microscopy.
Chronic cholestasis resulted in testicular atrophy evidenced by shrinkage and deformation of seminiferous tubules, thickening of peritubular boundaries, vacuolation, disorganization of germ cells, and maturation arrest. This was accompanied by decreased immunoreactivity of androgen receptors and proliferating cell nuclear antigen. Administration of ursodeoxycholic acid improved the testicular morphology and reversed cholestasis-induced immunohistochemical and ultrastructural changes.
Ursodeoxycholic acid can improve the testicular ultrastructure and restore the spermatogenic process in rats with chronic cholestasis. These findings support the clinical application of ursodeoxycholic acid in cholestatic patients especially those with hypogonadism.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2015.05.027</identifier><identifier>PMID: 26141989</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Androgen receptor ; Animals ; Cholagogues and Choleretics - pharmacology ; Cholagogues and Choleretics - therapeutic use ; Cholestasis ; Cholestasis - complications ; Cholestasis - drug therapy ; Cholestasis - metabolism ; Drug Evaluation, Preclinical ; Immunohistochemistry ; Male ; Proliferating cell nuclear antigen ; Rat ; Rats, Wistar ; Spermatogenesis - drug effects ; Testicular Diseases - etiology ; Testicular Diseases - metabolism ; Testicular Diseases - prevention & control ; Testis ; Testis - drug effects ; Testis - metabolism ; Testis - pathology ; Ultrastructure ; Ursodeoxycholic acid ; Ursodeoxycholic Acid - pharmacology ; Ursodeoxycholic Acid - therapeutic use</subject><ispartof>Life sciences (1973), 2015-09, Vol.136, p.52-59</ispartof><rights>2015</rights><rights>Copyright © 2015. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-9db4e404379824c11cef1d91cc5da788aed971824eb5afd0d9a27f8427c117bc3</citedby><cites>FETCH-LOGICAL-c353t-9db4e404379824c11cef1d91cc5da788aed971824eb5afd0d9a27f8427c117bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2015.05.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26141989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahmoud, Yomna I.</creatorcontrib><title>Testicular immunohistochemical and Ultrastructural changes associated with chronic cholestasis in rats: Effect of ursodeoxycholic acid</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Testicular atrophy has been commonly reported in patients with chronic liver diseases. Ursodeoxycholic acid is the most widely used drug for the treatment of many liver diseases. However, its effect on testicular ultrastructure associated with chronic cholestasis has never been studied. Thus, this study aimed to assess how chronic obstructive jaundice affects the testicular ultrastructure and whether it affects the androgen receptor or the proliferating cell nuclear antigen. The role of ursodeoxycholic acid was also investigated.
Cholestasis was induced by bile duct ligation. Samples were collected 4weeks postoperative. Testicular changes were assessed using immunohistochemistry and transmission electron microscopy.
Chronic cholestasis resulted in testicular atrophy evidenced by shrinkage and deformation of seminiferous tubules, thickening of peritubular boundaries, vacuolation, disorganization of germ cells, and maturation arrest. This was accompanied by decreased immunoreactivity of androgen receptors and proliferating cell nuclear antigen. Administration of ursodeoxycholic acid improved the testicular morphology and reversed cholestasis-induced immunohistochemical and ultrastructural changes.
Ursodeoxycholic acid can improve the testicular ultrastructure and restore the spermatogenic process in rats with chronic cholestasis. These findings support the clinical application of ursodeoxycholic acid in cholestatic patients especially those with hypogonadism.</description><subject>Androgen receptor</subject><subject>Animals</subject><subject>Cholagogues and Choleretics - pharmacology</subject><subject>Cholagogues and Choleretics - therapeutic use</subject><subject>Cholestasis</subject><subject>Cholestasis - complications</subject><subject>Cholestasis - drug therapy</subject><subject>Cholestasis - metabolism</subject><subject>Drug Evaluation, Preclinical</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Proliferating cell nuclear antigen</subject><subject>Rat</subject><subject>Rats, Wistar</subject><subject>Spermatogenesis - drug effects</subject><subject>Testicular Diseases - etiology</subject><subject>Testicular Diseases - metabolism</subject><subject>Testicular Diseases - prevention & control</subject><subject>Testis</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Ultrastructure</subject><subject>Ursodeoxycholic acid</subject><subject>Ursodeoxycholic Acid - pharmacology</subject><subject>Ursodeoxycholic Acid - therapeutic use</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9OHDEMhyNUBFvgAXqpcuxltknmT2baU4VoqYTEBc5R1nHYrGYmNM4UeIE-N1kt7bGSJUvO55-cj7EPUqylkN3n3Xr0tFZCtmtRSukjtpK9HirR1fIdWwmhmqpWoj1l74l2Qoi21fUJO1WdbOTQDyv25w4pB1hGm3iYpmWO20A5whanAHbkdnb8fszJUk4L5CWVGWzt_IDELVGEYDM6_hTytsxTnAOUHseSaikQDzNPNtMXfuU9QubR8yVRdBifX_ZcwS0Ed86OvR0JL976Gbv_fnV3eV3d3P74efntpoK6rXM1uE2DjWhqPfSqASkBvXSDBGid1X1v0Q1alifctNY74QartO8bpQurN1CfsU-H3McUfy3lSDMFAhxHO2NcyEgtulZ3jVIFlQcUUiRK6M1jCpNNL0YKs9dvdqboN3v9RpRSuux8fItfNhO6fxt_fRfg6wHA8snfAZMhCDgDupCKHuNi-E_8KzQ2mao</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Mahmoud, Yomna I.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150901</creationdate><title>Testicular immunohistochemical and Ultrastructural changes associated with chronic cholestasis in rats: Effect of ursodeoxycholic acid</title><author>Mahmoud, Yomna I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-9db4e404379824c11cef1d91cc5da788aed971824eb5afd0d9a27f8427c117bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Androgen receptor</topic><topic>Animals</topic><topic>Cholagogues and Choleretics - pharmacology</topic><topic>Cholagogues and Choleretics - therapeutic use</topic><topic>Cholestasis</topic><topic>Cholestasis - complications</topic><topic>Cholestasis - drug therapy</topic><topic>Cholestasis - metabolism</topic><topic>Drug Evaluation, Preclinical</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Proliferating cell nuclear antigen</topic><topic>Rat</topic><topic>Rats, Wistar</topic><topic>Spermatogenesis - drug effects</topic><topic>Testicular Diseases - etiology</topic><topic>Testicular Diseases - metabolism</topic><topic>Testicular Diseases - prevention & control</topic><topic>Testis</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Ultrastructure</topic><topic>Ursodeoxycholic acid</topic><topic>Ursodeoxycholic Acid - pharmacology</topic><topic>Ursodeoxycholic Acid - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahmoud, Yomna I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahmoud, Yomna I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Testicular immunohistochemical and Ultrastructural changes associated with chronic cholestasis in rats: Effect of ursodeoxycholic acid</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>136</volume><spage>52</spage><epage>59</epage><pages>52-59</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Testicular atrophy has been commonly reported in patients with chronic liver diseases. Ursodeoxycholic acid is the most widely used drug for the treatment of many liver diseases. However, its effect on testicular ultrastructure associated with chronic cholestasis has never been studied. Thus, this study aimed to assess how chronic obstructive jaundice affects the testicular ultrastructure and whether it affects the androgen receptor or the proliferating cell nuclear antigen. The role of ursodeoxycholic acid was also investigated.
Cholestasis was induced by bile duct ligation. Samples were collected 4weeks postoperative. Testicular changes were assessed using immunohistochemistry and transmission electron microscopy.
Chronic cholestasis resulted in testicular atrophy evidenced by shrinkage and deformation of seminiferous tubules, thickening of peritubular boundaries, vacuolation, disorganization of germ cells, and maturation arrest. This was accompanied by decreased immunoreactivity of androgen receptors and proliferating cell nuclear antigen. Administration of ursodeoxycholic acid improved the testicular morphology and reversed cholestasis-induced immunohistochemical and ultrastructural changes.
Ursodeoxycholic acid can improve the testicular ultrastructure and restore the spermatogenic process in rats with chronic cholestasis. These findings support the clinical application of ursodeoxycholic acid in cholestatic patients especially those with hypogonadism.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>26141989</pmid><doi>10.1016/j.lfs.2015.05.027</doi><tpages>8</tpages></addata></record> |
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| subjects | Androgen receptor Animals Cholagogues and Choleretics - pharmacology Cholagogues and Choleretics - therapeutic use Cholestasis Cholestasis - complications Cholestasis - drug therapy Cholestasis - metabolism Drug Evaluation, Preclinical Immunohistochemistry Male Proliferating cell nuclear antigen Rat Rats, Wistar Spermatogenesis - drug effects Testicular Diseases - etiology Testicular Diseases - metabolism Testicular Diseases - prevention & control Testis Testis - drug effects Testis - metabolism Testis - pathology Ultrastructure Ursodeoxycholic acid Ursodeoxycholic Acid - pharmacology Ursodeoxycholic Acid - therapeutic use |
| title | Testicular immunohistochemical and Ultrastructural changes associated with chronic cholestasis in rats: Effect of ursodeoxycholic acid |
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