Cholesterol overload in hepatocytes affects nicotinamide adenine dinucleotide phosphate oxidase (NADPH) activity abrogating hepatocyte growth factor (HGF) induced cellular protection

The increment in the prevalence of obesity incidence in Mexico is leading to the increase in many chronic maladies, including liver diseases. It is well known that lipid-induced liver sensitization is related to the kind of lipid rather than the amount of them in the organ. Cholesterol overload in t...

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Veröffentlicht in:Gaceta médica de México 2015-07, Vol.151 (4), p.456-464
Hauptverfasser: López-Reyes, Alberto G, Martínez-Flores, Karina, Clavijo-Cornejo, Denise, Nuño-Lámbarri, Natalia, Palestino-Domínguez, Mayrel, Souza, Verónica, Bucio, Leticia, Panduro, Arturo, Miranda, Roxana U, Gómez-Quiroz, Luis Enrique, Gutiérrez-Ruiz, María Concepción
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container_end_page 464
container_issue 4
container_start_page 456
container_title Gaceta médica de México
container_volume 151
creator López-Reyes, Alberto G
Martínez-Flores, Karina
Clavijo-Cornejo, Denise
Nuño-Lámbarri, Natalia
Palestino-Domínguez, Mayrel
Souza, Verónica
Bucio, Leticia
Panduro, Arturo
Miranda, Roxana U
Gómez-Quiroz, Luis Enrique
Gutiérrez-Ruiz, María Concepción
description The increment in the prevalence of obesity incidence in Mexico is leading to the increase in many chronic maladies, including liver diseases. It is well known that lipid-induced liver sensitization is related to the kind of lipid rather than the amount of them in the organ. Cholesterol overload in the liver aggravates the toxic effects of canonical liver insults. However, the status on the repair and survival response elicited by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and the hepatocyte growth factor (HGF) is not completely understood. In the present, work we aimed to figure out the HGF/NADPH oxidase-induced cellular protection in the hepatocyte with a cholesterol overload. Our results show that a hypercholesterolemic diet induced liver damage and steatosis in mice. The hepatocytes isolated from these animals exhibited an increase in basal NADPH oxidase activity, although transcriptional levels of some of its components were decreased. No effect on the oxidase activity was observed in HGF treatments. The protective effect of HGF was abrogated as a result of cholesterol cellular overload, calculated by a survival assay. In conclusion, the cholesterol overload in hepatocytes impairs the HGF/NADPH oxidase-induced cellular protection.
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subjects Animals
Cholesterol - metabolism
Hepatocyte Growth Factor - physiology
Hepatocytes - metabolism
Male
Mice
Mice, Inbred C57BL
NADPH Oxidases - physiology
title Cholesterol overload in hepatocytes affects nicotinamide adenine dinucleotide phosphate oxidase (NADPH) activity abrogating hepatocyte growth factor (HGF) induced cellular protection
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