Enzymatic synthesis of chitooligosaccharides in organic cosolvents
Diacetyl-chitobiose (GlcNAc) 2 was produced exclusively using a Vibrio furnissii chitinase ( chi E, 89 kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used f...
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| Veröffentlicht in: | Enzyme and microbial technology 2005-11, Vol.37 (6), p.663-668 |
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| container_title | Enzyme and microbial technology |
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| creator | Yoon, Jung Hae |
| description | Diacetyl-chitobiose (GlcNAc)
2 was produced exclusively using a
Vibrio furnissii chitinase (
chi E, 89
kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used for the efficient recycling of enzyme and synthesis of (GlcNAc)
2 on a preparative scale. Chitooligosaccharides were also synthesized in a transglycosylation reaction employing lysozyme from hen-egg white with diacetyl-chitobiose produced by a
Vibrio furnissii chitinase (
chi E, 89
kDa) as a substrate in 50% 2-ethoxy ethyl ether co-solvent system. The co-solvent systems studied were triethyl phosphate, 2-ethoxy ethyl ether and acetone. |
| doi_str_mv | 10.1016/j.enzmictec.2005.07.026 |
| format | Article |
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2 was produced exclusively using a
Vibrio furnissii chitinase (
chi E, 89
kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used for the efficient recycling of enzyme and synthesis of (GlcNAc)
2 on a preparative scale. Chitooligosaccharides were also synthesized in a transglycosylation reaction employing lysozyme from hen-egg white with diacetyl-chitobiose produced by a
Vibrio furnissii chitinase (
chi E, 89
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2 was produced exclusively using a
Vibrio furnissii chitinase (
chi E, 89
kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used for the efficient recycling of enzyme and synthesis of (GlcNAc)
2 on a preparative scale. Chitooligosaccharides were also synthesized in a transglycosylation reaction employing lysozyme from hen-egg white with diacetyl-chitobiose produced by a
Vibrio furnissii chitinase (
chi E, 89
kDa) as a substrate in 50% 2-ethoxy ethyl ether co-solvent system. The co-solvent systems studied were triethyl phosphate, 2-ethoxy ethyl ether and acetone.</description><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chitinases</subject><subject>Chitooligosaccharides</subject><subject>Enzymatic synthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lysozyme</subject><subject>Methods. Procedures. Technologies</subject><subject>Organic solvents</subject><subject>Vibrio furnissii</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkMtKBDEQRYMoOD6-wd7orttKP5LJUsUXCG50HWJ1xcnQk2iqFcavt3VEl64KinPrUkeIIwmVBKlOlxXFj1XAkbCqAboKdAW12hIzOdemBANmW8xAtrKEuja7Yo95CTAtWpiJ88v4sV65MWDB6zguiAMXyRe4CGNKQ3hO7BAXLoeeuAixSPnZxYnGxGl4pzjygdjxbmA6_Jn74vHq8uHipry7v769OLsrsdFqLOdtVztj2kaBQqdR-Ua3xpB2qFswnZfOP_Vd72nek6-VrvsG3bwhcPLJdKrZFyebuy85vb4Rj3YVGGkYXKT0xlZq6FT7DeoNiDkxZ_L2JYeVy2srwX45s0v768x-ObOg7eRsSh7_VDhGN_jsIgb-i2tZN0p3E3e24Wj69z1QtoyBIlIfMuFo-xT-7foEtguH9g</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Yoon, Jung Hae</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20051101</creationdate><title>Enzymatic synthesis of chitooligosaccharides in organic cosolvents</title><author>Yoon, Jung Hae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-8452a9943606ca7c6f37499e7ac74095f1afbd5dfe8def2672d3ca83e0a1b9563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chitinases</topic><topic>Chitooligosaccharides</topic><topic>Enzymatic synthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lysozyme</topic><topic>Methods. Procedures. Technologies</topic><topic>Organic solvents</topic><topic>Vibrio furnissii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoon, Jung Hae</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoon, Jung Hae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic synthesis of chitooligosaccharides in organic cosolvents</atitle><jtitle>Enzyme and microbial technology</jtitle><date>2005-11-01</date><risdate>2005</risdate><volume>37</volume><issue>6</issue><spage>663</spage><epage>668</epage><pages>663-668</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>Diacetyl-chitobiose (GlcNAc)
2 was produced exclusively using a
Vibrio furnissii chitinase (
chi E, 89
kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used for the efficient recycling of enzyme and synthesis of (GlcNAc)
2 on a preparative scale. Chitooligosaccharides were also synthesized in a transglycosylation reaction employing lysozyme from hen-egg white with diacetyl-chitobiose produced by a
Vibrio furnissii chitinase (
chi E, 89
kDa) as a substrate in 50% 2-ethoxy ethyl ether co-solvent system. The co-solvent systems studied were triethyl phosphate, 2-ethoxy ethyl ether and acetone.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/j.enzmictec.2005.07.026</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 2005-11, Vol.37 (6), p.663-668 |
| issn | 0141-0229 1879-0909 |
| language | eng |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Chitinases Chitooligosaccharides Enzymatic synthesis Fundamental and applied biological sciences. Psychology Lysozyme Methods. Procedures. Technologies Organic solvents Vibrio furnissii |
| title | Enzymatic synthesis of chitooligosaccharides in organic cosolvents |
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