Properties and Anti-Hiv Activity of Hairpin Antisense Oligonucleotides Containing 2′-Methoxynucleosides with Base-Pairing in the Stem Region at the 3′-End
A new type of hairpin antisense oligodeoxyribo-nucleotide, containing 2′-methoxynucleosides with base-pairing in the stem region at the 3′-end, was tested for 3′-exonuclease resistance and anti-HIV activity. An increased resistance to nuclease degradation has been observed by incubation of the hairp...
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| Veröffentlicht in: | Antiviral chemistry & chemotherapy 1996-04, Vol.7 (2), p.86-93 |
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| container_title | Antiviral chemistry & chemotherapy |
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| creator | Hosono, K. Kuwasaki, T. Inagawa, T. Takai, K. Nakashima, H. Saito, T. Yamamoto, N. Takaku, H. |
| description | A new type of hairpin antisense oligodeoxyribo-nucleotide, containing 2′-methoxynucleosides with base-pairing in the stem region at the 3′-end, was tested for 3′-exonuclease resistance and anti-HIV activity. An increased resistance to nuclease degradation has been observed by incubation of the hairpin oligo-nucleotides with DNA polymerase and foetal bovine serum. Of particular interest is the hairpin antisense oligonucleotide containing 2′-methoxynucleosides with base-pairing in the stem region at the 3′-end, which has increased nuclease resistance and hybridizes effectively with a complementary RNA. Furthermore, these compounds were assayed for inhibition of virus replication in HIV-1 infected MT-4 cells. In the anti-HIV activity test, the hairpin oligonucleotide phosphorothioate derivatives showed higher anti-HIV activities compared to their linear counterparts. |
| doi_str_mv | 10.1177/095632029600700205 |
| format | Article |
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An increased resistance to nuclease degradation has been observed by incubation of the hairpin oligo-nucleotides with DNA polymerase and foetal bovine serum. Of particular interest is the hairpin antisense oligonucleotide containing 2′-methoxynucleosides with base-pairing in the stem region at the 3′-end, which has increased nuclease resistance and hybridizes effectively with a complementary RNA. Furthermore, these compounds were assayed for inhibition of virus replication in HIV-1 infected MT-4 cells. In the anti-HIV activity test, the hairpin oligonucleotide phosphorothioate derivatives showed higher anti-HIV activities compared to their linear counterparts.</description><identifier>ISSN: 2040-2066</identifier><identifier>ISSN: 0956-3202</identifier><identifier>EISSN: 2040-2066</identifier><identifier>DOI: 10.1177/095632029600700205</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Antibiotics. Antiinfectious agents. 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Antiparasitic agents</subject><subject>Antisense oligonucleotides</subject><subject>Antiviral activity</subject><subject>Antiviral agents</subject><subject>Biological and medical sciences</subject><subject>DNA-directed DNA polymerase</subject><subject>Exonuclease</subject><subject>HIV</subject><subject>Human immunodeficiency virus</subject><subject>human immunodeficiency virus 1</subject><subject>Medical sciences</subject><subject>Nuclease</subject><subject>Nucleotides</subject><subject>Pharmacology. 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An increased resistance to nuclease degradation has been observed by incubation of the hairpin oligo-nucleotides with DNA polymerase and foetal bovine serum. Of particular interest is the hairpin antisense oligonucleotide containing 2′-methoxynucleosides with base-pairing in the stem region at the 3′-end, which has increased nuclease resistance and hybridizes effectively with a complementary RNA. Furthermore, these compounds were assayed for inhibition of virus replication in HIV-1 infected MT-4 cells. In the anti-HIV activity test, the hairpin oligonucleotide phosphorothioate derivatives showed higher anti-HIV activities compared to their linear counterparts.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><doi>10.1177/095632029600700205</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2040-2066 |
| ispartof | Antiviral chemistry & chemotherapy, 1996-04, Vol.7 (2), p.86-93 |
| issn | 2040-2066 0956-3202 2040-2066 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antibiotics. Antiinfectious agents. Antiparasitic agents Antisense oligonucleotides Antiviral activity Antiviral agents Biological and medical sciences DNA-directed DNA polymerase Exonuclease HIV Human immunodeficiency virus human immunodeficiency virus 1 Medical sciences Nuclease Nucleotides Pharmacology. Drug treatments Phosphorothioate Ribonucleic acid RNA |
| title | Properties and Anti-Hiv Activity of Hairpin Antisense Oligonucleotides Containing 2′-Methoxynucleosides with Base-Pairing in the Stem Region at the 3′-End |
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