Identification of tetrodotoxin and paralytic shellfish toxins in marine gastropods [Niotha clathrata and Zeuxis scalaris] implicated in food poisoning [incidents in south Taiwan]
Attempts were made to elucidate the responsible toxins in the gastropod Niotha clathrata and Zeuxis scalaris which recently caused food poisoning incidents in south Taiwan. The remained (cooked) and captured (live) specimens of both gastropods were assayed for toxicity (as tetrodotoxin = TTX). The a...
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| Veröffentlicht in: | Fisheries science 1995, Vol.61 (4), p.675-679 |
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| creator | Hwang, D.F. (National Taiwan Ocean Univ., Keelung) Cheng, C.A Tsai, H.T Shih, D.Y.C Ko, H.C Yang, R.Z Jeng, S.S |
| description | Attempts were made to elucidate the responsible toxins in the gastropod Niotha clathrata and Zeuxis scalaris which recently caused food poisoning incidents in south Taiwan. The remained (cooked) and captured (live) specimens of both gastropods were assayed for toxicity (as tetrodotoxin = TTX). The average toxicity of cooked and live specimens was 150 plus or minus 126 (mean plus or minus S.D.) and 345 plus or minus 192 mouse unit (MU) in N. clathrata, and 13 plus or minus 9 and 98 plus or minus 46 MU in Z. scalaris, respectively. The toxin was partially purified from the methanolic extract of the gastropod by ultrafiltration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis, TLC, and HPLC analyses demonstrated that the toxin consisted mainly of TTX (about 80%), along with minor paralytic shellfish poison (PSP, about 20%). The PSP components were identified as gonyautoxin 1-4 and neosaxitoxin in both gastropods. It was concluded from these results that the causative agents of the above food poisoning were TTX and PSP. |
| doi_str_mv | 10.2331/fishsci.61.675 |
| format | Article |
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(National Taiwan Ocean Univ., Keelung) ; Cheng, C.A ; Tsai, H.T ; Shih, D.Y.C ; Ko, H.C ; Yang, R.Z ; Jeng, S.S</creator><creatorcontrib>Hwang, D.F. (National Taiwan Ocean Univ., Keelung) ; Cheng, C.A ; Tsai, H.T ; Shih, D.Y.C ; Ko, H.C ; Yang, R.Z ; Jeng, S.S</creatorcontrib><description>Attempts were made to elucidate the responsible toxins in the gastropod Niotha clathrata and Zeuxis scalaris which recently caused food poisoning incidents in south Taiwan. The remained (cooked) and captured (live) specimens of both gastropods were assayed for toxicity (as tetrodotoxin = TTX). The average toxicity of cooked and live specimens was 150 plus or minus 126 (mean plus or minus S.D.) and 345 plus or minus 192 mouse unit (MU) in N. clathrata, and 13 plus or minus 9 and 98 plus or minus 46 MU in Z. scalaris, respectively. The toxin was partially purified from the methanolic extract of the gastropod by ultrafiltration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis, TLC, and HPLC analyses demonstrated that the toxin consisted mainly of TTX (about 80%), along with minor paralytic shellfish poison (PSP, about 20%). The PSP components were identified as gonyautoxin 1-4 and neosaxitoxin in both gastropods. 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The average toxicity of cooked and live specimens was 150 plus or minus 126 (mean plus or minus S.D.) and 345 plus or minus 192 mouse unit (MU) in N. clathrata, and 13 plus or minus 9 and 98 plus or minus 46 MU in Z. scalaris, respectively. The toxin was partially purified from the methanolic extract of the gastropod by ultrafiltration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis, TLC, and HPLC analyses demonstrated that the toxin consisted mainly of TTX (about 80%), along with minor paralytic shellfish poison (PSP, about 20%). The PSP components were identified as gonyautoxin 1-4 and neosaxitoxin in both gastropods. It was concluded from these results that the causative agents of the above food poisoning were TTX and PSP.</description><subject>COMPLEXACION</subject><subject>COMPLEXATION</subject><subject>COMPLEXING</subject><subject>COQUILLAGES ET CRUSTACES</subject><subject>ENVENENAMIENTO</subject><subject>GASTROPODA</subject><subject>INTOXICATION</subject><subject>Marine</subject><subject>MARISCOS</subject><subject>MORBIDITE</subject><subject>MORBIDITY</subject><subject>MORBOSIDAD</subject><subject>Niotha clathrata</subject><subject>PARALISIS</subject><subject>PARALYSIE</subject><subject>PARALYSIS</subject><subject>POISONING</subject><subject>POISSON ARME</subject><subject>PUFFERS (FISH)</subject><subject>SHELLFISH</subject><subject>TAIWAN</subject><subject>TAMBOR (PEZ)</subject><subject>TOXINAS</subject><subject>TOXINE</subject><subject>TOXINS</subject><issn>0919-9268</issn><issn>1444-2906</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNotUU1v1DAQtRCVWEqvHDj5xG0X24mdzRFVUIoq4NBeWlXWxHY2g7J2yHhF-7f4hTi7Pc1h3sfMe4y9l2Kjqkp-6pEGcrgxcmMa_YqtZF3Xa9UK85qtRCvbdavM9g17S_RbCGG02K7Yv2sfYsYeHWRMkaee55Dn5FNOTxg5RM8nmGF8zug4DWEcFx9-3BIviD3MGAPfARXalDzxhx-Y8gDcjZCHGTIcVe7D4QmJk4OxMOiR434aF9vgF5k-peKUkFLEuOMPGB0upx09KB3ywG8B_0J8fMfOehgpXLzMc3b39cvt5bf1zc-r68vPN2unlczL49W2abX2Sqi-dtq30jddHaQB2enKKCM0NGEr2zq4UAnfCQ8KOtF2TaVUdc4-nnSnOf05BMp2j-RKABBDOpCVjdBSq6YANyegmxPRHHo7zVhiebZS2KUa-1KNNdKWagrhw4nQQ7KwK2nY779aIxpZ1P4D71WQ5A</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Hwang, D.F. 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(National Taiwan Ocean Univ., Keelung)</creatorcontrib><creatorcontrib>Cheng, C.A</creatorcontrib><creatorcontrib>Tsai, H.T</creatorcontrib><creatorcontrib>Shih, D.Y.C</creatorcontrib><creatorcontrib>Ko, H.C</creatorcontrib><creatorcontrib>Yang, R.Z</creatorcontrib><creatorcontrib>Jeng, S.S</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Fisheries science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, D.F. (National Taiwan Ocean Univ., Keelung)</au><au>Cheng, C.A</au><au>Tsai, H.T</au><au>Shih, D.Y.C</au><au>Ko, H.C</au><au>Yang, R.Z</au><au>Jeng, S.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of tetrodotoxin and paralytic shellfish toxins in marine gastropods [Niotha clathrata and Zeuxis scalaris] implicated in food poisoning [incidents in south Taiwan]</atitle><jtitle>Fisheries science</jtitle><date>1995</date><risdate>1995</risdate><volume>61</volume><issue>4</issue><spage>675</spage><epage>679</epage><pages>675-679</pages><issn>0919-9268</issn><eissn>1444-2906</eissn><abstract>Attempts were made to elucidate the responsible toxins in the gastropod Niotha clathrata and Zeuxis scalaris which recently caused food poisoning incidents in south Taiwan. The remained (cooked) and captured (live) specimens of both gastropods were assayed for toxicity (as tetrodotoxin = TTX). The average toxicity of cooked and live specimens was 150 plus or minus 126 (mean plus or minus S.D.) and 345 plus or minus 192 mouse unit (MU) in N. clathrata, and 13 plus or minus 9 and 98 plus or minus 46 MU in Z. scalaris, respectively. The toxin was partially purified from the methanolic extract of the gastropod by ultrafiltration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis, TLC, and HPLC analyses demonstrated that the toxin consisted mainly of TTX (about 80%), along with minor paralytic shellfish poison (PSP, about 20%). The PSP components were identified as gonyautoxin 1-4 and neosaxitoxin in both gastropods. It was concluded from these results that the causative agents of the above food poisoning were TTX and PSP.</abstract><doi>10.2331/fishsci.61.675</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fisheries science, 1995, Vol.61 (4), p.675-679 |
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| source | J-STAGE Free; EZB-FREE-00999 freely available EZB journals |
| subjects | COMPLEXACION COMPLEXATION COMPLEXING COQUILLAGES ET CRUSTACES ENVENENAMIENTO GASTROPODA INTOXICATION Marine MARISCOS MORBIDITE MORBIDITY MORBOSIDAD Niotha clathrata PARALISIS PARALYSIE PARALYSIS POISONING POISSON ARME PUFFERS (FISH) SHELLFISH TAIWAN TAMBOR (PEZ) TOXINAS TOXINE TOXINS |
| title | Identification of tetrodotoxin and paralytic shellfish toxins in marine gastropods [Niotha clathrata and Zeuxis scalaris] implicated in food poisoning [incidents in south Taiwan] |
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