Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis
Sauvagesia rhodoleuca (Ochnaceae) is an endangered plant that is endemic to southern China. The levels of genetic variation and patterns of population structure in S. rhodoleuca were investigated using inter-simple sequence repeat markers. Eleven primers were used to amplify DNA samples from 117 ind...
Gespeichert in:
| Veröffentlicht in: | Genetics and molecular research 2014-10, Vol.13 (4), p.8258-8267 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8267 |
|---|---|
| container_issue | 4 |
| container_start_page | 8258 |
| container_title | Genetics and molecular research |
| container_volume | 13 |
| creator | Chai, S F Jiang, Y S Zhuang, X Y Shi, Y C Wei, X Luo, W H Chen, Z Y |
| description | Sauvagesia rhodoleuca (Ochnaceae) is an endangered plant that is endemic to southern China. The levels of genetic variation and patterns of population structure in S. rhodoleuca were investigated using inter-simple sequence repeat markers. Eleven primers were used to amplify DNA samples from 117 individuals, and a total of 92 loci were detected. Our results indicated that genetic diversity was quite low both at the species level (percentage of polymorphic bands (PPB) = 41.30%, Nei's gene diversity (h) = 0.1331, and Shannon information index (I) = 0.2028) and the population level (PPB = 16.30-28.26%, h = 0.0496-0.1012, and I = 0.0756-0.1508). A high level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (0.4344) and analysis of molecular variance (47.03%). The low genetic diversity within population and high population differentiation of S. rhodoleuca were assumed to result largely from limited gene flow, genetic drift, inbreeding, and clonal growth. Conservation strategies for this endangered species are proposed based on the genetic data. |
| doi_str_mv | 10.4238/2014.October.20.2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1705086371</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1705086371</sourcerecordid><originalsourceid>FETCH-LOGICAL-c329t-33b96db78435f173e4678a69d50412ab9b0115e54632864cba39257aea19f29f3</originalsourceid><addsrcrecordid>eNqFkb1OwzAUhS0E4v8BWJBHlhb_xE4yogoKEhIShTm6sW9ao9QptoPUJ-C1SWlBbEz20T3fWT5CLjgbZ0IW14LxbPxkUldjGAs2FnvkmOtcj5Qu2P6f_xE5ifGNMaGygh2SI6Gk1rlgx-Rzih6TM9S6DwzRpTUFb4fUNBjQJwfJdZ52DU0LpOgt-PlwsN-tIeJyYOMKjcNIZ9B_wByjAxoWne1a7A1Q5-lk4TxQiNRiQpMGvF7Th9nseZiBdh1dPCMHDbQRz3fvKXm9u32Z3I8en6YPk5vHkZGiTCMp61LbOi8yqRqeS8x0XoAurWIZF1CXNeNcocq0FIXOTA2yFCoHBF42omzkKbna7q5C995jTNXSRYNtCx67PlY8Z4oVWub8_6oWTBWaiU2Vb6smdDEGbKpVcEsI64qzaqOq2qiqdqqGUImBudzN9_US7S_x40Z-ATwOkXA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1620586021</pqid></control><display><type>article</type><title>Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Chai, S F ; Jiang, Y S ; Zhuang, X Y ; Shi, Y C ; Wei, X ; Luo, W H ; Chen, Z Y</creator><creatorcontrib>Chai, S F ; Jiang, Y S ; Zhuang, X Y ; Shi, Y C ; Wei, X ; Luo, W H ; Chen, Z Y</creatorcontrib><description>Sauvagesia rhodoleuca (Ochnaceae) is an endangered plant that is endemic to southern China. The levels of genetic variation and patterns of population structure in S. rhodoleuca were investigated using inter-simple sequence repeat markers. Eleven primers were used to amplify DNA samples from 117 individuals, and a total of 92 loci were detected. Our results indicated that genetic diversity was quite low both at the species level (percentage of polymorphic bands (PPB) = 41.30%, Nei's gene diversity (h) = 0.1331, and Shannon information index (I) = 0.2028) and the population level (PPB = 16.30-28.26%, h = 0.0496-0.1012, and I = 0.0756-0.1508). A high level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (0.4344) and analysis of molecular variance (47.03%). The low genetic diversity within population and high population differentiation of S. rhodoleuca were assumed to result largely from limited gene flow, genetic drift, inbreeding, and clonal growth. Conservation strategies for this endangered species are proposed based on the genetic data.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/2014.October.20.2</identifier><identifier>PMID: 25366720</identifier><language>eng</language><publisher>Brazil</publisher><subject>China ; Endangered Species ; Evolution, Molecular ; Genetic Variation ; Genetics, Population ; Geography ; Introduced Species ; Microsatellite Repeats ; Ochnaceae ; Ochnaceae - genetics ; Sauvagesia</subject><ispartof>Genetics and molecular research, 2014-10, Vol.13 (4), p.8258-8267</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25366720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chai, S F</creatorcontrib><creatorcontrib>Jiang, Y S</creatorcontrib><creatorcontrib>Zhuang, X Y</creatorcontrib><creatorcontrib>Shi, Y C</creatorcontrib><creatorcontrib>Wei, X</creatorcontrib><creatorcontrib>Luo, W H</creatorcontrib><creatorcontrib>Chen, Z Y</creatorcontrib><title>Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Sauvagesia rhodoleuca (Ochnaceae) is an endangered plant that is endemic to southern China. The levels of genetic variation and patterns of population structure in S. rhodoleuca were investigated using inter-simple sequence repeat markers. Eleven primers were used to amplify DNA samples from 117 individuals, and a total of 92 loci were detected. Our results indicated that genetic diversity was quite low both at the species level (percentage of polymorphic bands (PPB) = 41.30%, Nei's gene diversity (h) = 0.1331, and Shannon information index (I) = 0.2028) and the population level (PPB = 16.30-28.26%, h = 0.0496-0.1012, and I = 0.0756-0.1508). A high level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (0.4344) and analysis of molecular variance (47.03%). The low genetic diversity within population and high population differentiation of S. rhodoleuca were assumed to result largely from limited gene flow, genetic drift, inbreeding, and clonal growth. Conservation strategies for this endangered species are proposed based on the genetic data.</description><subject>China</subject><subject>Endangered Species</subject><subject>Evolution, Molecular</subject><subject>Genetic Variation</subject><subject>Genetics, Population</subject><subject>Geography</subject><subject>Introduced Species</subject><subject>Microsatellite Repeats</subject><subject>Ochnaceae</subject><subject>Ochnaceae - genetics</subject><subject>Sauvagesia</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1OwzAUhS0E4v8BWJBHlhb_xE4yogoKEhIShTm6sW9ao9QptoPUJ-C1SWlBbEz20T3fWT5CLjgbZ0IW14LxbPxkUldjGAs2FnvkmOtcj5Qu2P6f_xE5ifGNMaGygh2SI6Gk1rlgx-Rzih6TM9S6DwzRpTUFb4fUNBjQJwfJdZ52DU0LpOgt-PlwsN-tIeJyYOMKjcNIZ9B_wByjAxoWne1a7A1Q5-lk4TxQiNRiQpMGvF7Th9nseZiBdh1dPCMHDbQRz3fvKXm9u32Z3I8en6YPk5vHkZGiTCMp61LbOi8yqRqeS8x0XoAurWIZF1CXNeNcocq0FIXOTA2yFCoHBF42omzkKbna7q5C995jTNXSRYNtCx67PlY8Z4oVWub8_6oWTBWaiU2Vb6smdDEGbKpVcEsI64qzaqOq2qiqdqqGUImBudzN9_US7S_x40Z-ATwOkXA</recordid><startdate>20141020</startdate><enddate>20141020</enddate><creator>Chai, S F</creator><creator>Jiang, Y S</creator><creator>Zhuang, X Y</creator><creator>Shi, Y C</creator><creator>Wei, X</creator><creator>Luo, W H</creator><creator>Chen, Z Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20141020</creationdate><title>Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis</title><author>Chai, S F ; Jiang, Y S ; Zhuang, X Y ; Shi, Y C ; Wei, X ; Luo, W H ; Chen, Z Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-33b96db78435f173e4678a69d50412ab9b0115e54632864cba39257aea19f29f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>China</topic><topic>Endangered Species</topic><topic>Evolution, Molecular</topic><topic>Genetic Variation</topic><topic>Genetics, Population</topic><topic>Geography</topic><topic>Introduced Species</topic><topic>Microsatellite Repeats</topic><topic>Ochnaceae</topic><topic>Ochnaceae - genetics</topic><topic>Sauvagesia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chai, S F</creatorcontrib><creatorcontrib>Jiang, Y S</creatorcontrib><creatorcontrib>Zhuang, X Y</creatorcontrib><creatorcontrib>Shi, Y C</creatorcontrib><creatorcontrib>Wei, X</creatorcontrib><creatorcontrib>Luo, W H</creatorcontrib><creatorcontrib>Chen, Z Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chai, S F</au><au>Jiang, Y S</au><au>Zhuang, X Y</au><au>Shi, Y C</au><au>Wei, X</au><au>Luo, W H</au><au>Chen, Z Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2014-10-20</date><risdate>2014</risdate><volume>13</volume><issue>4</issue><spage>8258</spage><epage>8267</epage><pages>8258-8267</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>Sauvagesia rhodoleuca (Ochnaceae) is an endangered plant that is endemic to southern China. The levels of genetic variation and patterns of population structure in S. rhodoleuca were investigated using inter-simple sequence repeat markers. Eleven primers were used to amplify DNA samples from 117 individuals, and a total of 92 loci were detected. Our results indicated that genetic diversity was quite low both at the species level (percentage of polymorphic bands (PPB) = 41.30%, Nei's gene diversity (h) = 0.1331, and Shannon information index (I) = 0.2028) and the population level (PPB = 16.30-28.26%, h = 0.0496-0.1012, and I = 0.0756-0.1508). A high level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (0.4344) and analysis of molecular variance (47.03%). The low genetic diversity within population and high population differentiation of S. rhodoleuca were assumed to result largely from limited gene flow, genetic drift, inbreeding, and clonal growth. Conservation strategies for this endangered species are proposed based on the genetic data.</abstract><cop>Brazil</cop><pmid>25366720</pmid><doi>10.4238/2014.October.20.2</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1676-5680 |
| ispartof | Genetics and molecular research, 2014-10, Vol.13 (4), p.8258-8267 |
| issn | 1676-5680 1676-5680 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1705086371 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | China Endangered Species Evolution, Molecular Genetic Variation Genetics, Population Geography Introduced Species Microsatellite Repeats Ochnaceae Ochnaceae - genetics Sauvagesia |
| title | Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T16%3A35%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20diversity%20and%20differentiation%20of%20the%20endangered%20and%20endemic%20species%20Sauvagesia%20rhodoleuca%20in%20China%20as%20detected%20by%20ISSR%20analysis&rft.jtitle=Genetics%20and%20molecular%20research&rft.au=Chai,%20S%20F&rft.date=2014-10-20&rft.volume=13&rft.issue=4&rft.spage=8258&rft.epage=8267&rft.pages=8258-8267&rft.issn=1676-5680&rft.eissn=1676-5680&rft_id=info:doi/10.4238/2014.October.20.2&rft_dat=%3Cproquest_cross%3E1705086371%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1620586021&rft_id=info:pmid/25366720&rfr_iscdi=true |