Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8
[Display omitted] l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 2015-09, Vol.25 (18), p.3980-3983 |
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| container_title | Bioorganic & medicinal chemistry letters |
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| creator | Li, Zijie Wu, Xiaoru Cai, Li Duan, Shenglin Liu, Jia Yuan, Peng Nakanishi, Hideki Gao, Xiao-Dong |
| description | [Display omitted]
l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars d-psicose, d-sorbose, l-tagatose and l-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation. |
| doi_str_mv | 10.1016/j.bmcl.2015.07.027 |
| format | Article |
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l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars d-psicose, d-sorbose, l-tagatose and l-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/j.bmcl.2015.07.027</identifier><identifier>PMID: 26227774</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aldehyde-Lyases - metabolism ; Aldolase ; Fructose - biosynthesis ; Fructose - chemistry ; Hexoses - biosynthesis ; Hexoses - chemistry ; Molecular Structure ; Rare sugar ; RhaD ; Sorbose - biosynthesis ; Sorbose - chemistry ; Synthesis ; Thermotoga maritima ; Thermotoga maritima - enzymology</subject><ispartof>Bioorganic & medicinal chemistry letters, 2015-09, Vol.25 (18), p.3980-3983</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-b0a74cfa8da591f2f68336752bff091e1cc0fcf2d5c04f79d0c66d64f62ae46d3</citedby><cites>FETCH-LOGICAL-c356t-b0a74cfa8da591f2f68336752bff091e1cc0fcf2d5c04f79d0c66d64f62ae46d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960894X15007313$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26227774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zijie</creatorcontrib><creatorcontrib>Wu, Xiaoru</creatorcontrib><creatorcontrib>Cai, Li</creatorcontrib><creatorcontrib>Duan, Shenglin</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Yuan, Peng</creatorcontrib><creatorcontrib>Nakanishi, Hideki</creatorcontrib><creatorcontrib>Gao, Xiao-Dong</creatorcontrib><title>Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>[Display omitted]
l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars d-psicose, d-sorbose, l-tagatose and l-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.</description><subject>Aldehyde-Lyases - metabolism</subject><subject>Aldolase</subject><subject>Fructose - biosynthesis</subject><subject>Fructose - chemistry</subject><subject>Hexoses - biosynthesis</subject><subject>Hexoses - chemistry</subject><subject>Molecular Structure</subject><subject>Rare sugar</subject><subject>RhaD</subject><subject>Sorbose - biosynthesis</subject><subject>Sorbose - chemistry</subject><subject>Synthesis</subject><subject>Thermotoga maritima</subject><subject>Thermotoga maritima - enzymology</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAURS0EotPCD7BAXrJJeHYceyKxgapQpKIu2krsLI_9PPEoiQfbKZp-PRlNYcnqbc69uu8Q8o5BzYDJj7t6M9qh5sDaGlQNXL0gKyakqBoB7Uuygk5Cte7EzzNynvMOgAkQ4jU545JzpZRYke3V9HQYTQmW5sNUeswh0-hpMglpnrcmZfo7lJ4OVerNOM1DzFixat_HvO9NQWoGFweTkfoUR3rfYxpjiVtDR5NCCaOhP-6-rN-QV94MGd8-3wvy8PXq_vK6urn99v3y801lm1aWagNGCevN2pm2Y557uW4aqVq-8R46hsxa8NZz11oQXnUOrJROCi-5QSFdc0E-nHr3Kf6aMRc9hmxxGMyEcc6aKWgBOgCxoPyE2hRzTuj1Pi1z00Ez0EfBeqePgvVRsAalF8FL6P1z_7wZ0f2L_DW6AJ9OAC5fPgZMOtuAk0UXEtqiXQz_6_8D3K6ODg</recordid><startdate>20150915</startdate><enddate>20150915</enddate><creator>Li, Zijie</creator><creator>Wu, Xiaoru</creator><creator>Cai, Li</creator><creator>Duan, Shenglin</creator><creator>Liu, Jia</creator><creator>Yuan, Peng</creator><creator>Nakanishi, Hideki</creator><creator>Gao, Xiao-Dong</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150915</creationdate><title>Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8</title><author>Li, Zijie ; Wu, Xiaoru ; Cai, Li ; Duan, Shenglin ; Liu, Jia ; Yuan, Peng ; Nakanishi, Hideki ; Gao, Xiao-Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-b0a74cfa8da591f2f68336752bff091e1cc0fcf2d5c04f79d0c66d64f62ae46d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aldehyde-Lyases - metabolism</topic><topic>Aldolase</topic><topic>Fructose - biosynthesis</topic><topic>Fructose - chemistry</topic><topic>Hexoses - biosynthesis</topic><topic>Hexoses - chemistry</topic><topic>Molecular Structure</topic><topic>Rare sugar</topic><topic>RhaD</topic><topic>Sorbose - biosynthesis</topic><topic>Sorbose - chemistry</topic><topic>Synthesis</topic><topic>Thermotoga maritima</topic><topic>Thermotoga maritima - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zijie</creatorcontrib><creatorcontrib>Wu, Xiaoru</creatorcontrib><creatorcontrib>Cai, Li</creatorcontrib><creatorcontrib>Duan, Shenglin</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Yuan, Peng</creatorcontrib><creatorcontrib>Nakanishi, Hideki</creatorcontrib><creatorcontrib>Gao, Xiao-Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zijie</au><au>Wu, Xiaoru</au><au>Cai, Li</au><au>Duan, Shenglin</au><au>Liu, Jia</au><au>Yuan, Peng</au><au>Nakanishi, Hideki</au><au>Gao, Xiao-Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2015-09-15</date><risdate>2015</risdate><volume>25</volume><issue>18</issue><spage>3980</spage><epage>3983</epage><pages>3980-3983</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>[Display omitted]
l-Rhamnulose-1-phosphate aldolase from a thermophilic source (Thermotoga maritima MSB8) (RhaDT.mari) was heterologously overexpressed in Escherichia coli and the stereoselectivity of this enzyme with or without Nus tag was investigated. We also applied this enzyme to the synthesis of rare sugars d-psicose, d-sorbose, l-tagatose and l-fructose using our one-pot four-enzyme system. To the best of our knowledge, this is the first use of RhaD from a thermophilic source for rare sugar synthesis and the temperature tolerance of this enzyme paves the path for large scale fermentation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26227774</pmid><doi>10.1016/j.bmcl.2015.07.027</doi><tpages>4</tpages></addata></record> |
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| ispartof | Bioorganic & medicinal chemistry letters, 2015-09, Vol.25 (18), p.3980-3983 |
| issn | 0960-894X 1464-3405 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aldehyde-Lyases - metabolism Aldolase Fructose - biosynthesis Fructose - chemistry Hexoses - biosynthesis Hexoses - chemistry Molecular Structure Rare sugar RhaD Sorbose - biosynthesis Sorbose - chemistry Synthesis Thermotoga maritima Thermotoga maritima - enzymology |
| title | Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritima MSB8 |
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