Cryopreserved and hypothermically stored rat liver parenchymal cells as metabolizing system in the Salmonella mutagenicity assay

Freshly isolated and preserved rat liver parenchymal cells were used as metabolizing system in the Salmonella mutagenicity assay. The liver cells were isolated with EDTA perfusion without the addition of collagenase and had a viability of 96% as judged by trypan blue exclusion. When freshly isolated...

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Veröffentlicht in:	Mutation Research-Environmental Mutagenesis and Related Subjects 1995-12, Vol.335 (3), p.309-316
Hauptverfasser:	Diener, Bernd, Oesch, Franz
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Allopurinol Animals Biological and medical sciences Biotransformation Cell mediated Cell Survival Cryopreservation General aspects. Methods Glutathione Hepatocyte Insulin Liver - cytology Liver - metabolism Male Medical sciences Mutagenicity Tests - methods Organ Preservation Solutions Raffinose Rats Rats, Sprague-Dawley Salmonella mutagenicity assay Salmonella typhimurium Salmonella typhimurium - drug effects Salmonella typhimurium - genetics Toxicology University of wisconsin solution
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