Preparation of a Monoclonal Antibody Specific to Chum Salmon Growth Hormone

Preparation of a Monoclonal Antibody Specific to Chum Salmon Growth Hormone

We have prepared a monoclonal antibody (MAb) specific to chum salmon (Oncorhynchus keta) growth hormone (GH). The MAb only reacted with GH among pituitary hormones of chum salmon. Moreover, the MAb also reacted with GHs from coho salmon, chinook salmon, bonito, and tuna, but there are no cross-react...

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Veröffentlicht in:Fisheries science 1995, Vol.61 (4), p.718-719
Hauptverfasser: Nishii, Manabu, Koide, Yoshio, Hishinuma, Takanori, Mizugaki, Michinao
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Oncorhynchus keta
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container_end_page 719
container_issue 4
container_start_page 718
container_title Fisheries science
container_volume 61
creator Nishii, Manabu
Koide, Yoshio
Hishinuma, Takanori
Mizugaki, Michinao
description We have prepared a monoclonal antibody (MAb) specific to chum salmon (Oncorhynchus keta) growth hormone (GH). The MAb only reacted with GH among pituitary hormones of chum salmon. Moreover, the MAb also reacted with GHs from coho salmon, chinook salmon, bonito, and tuna, but there are no cross-reactivity with GHs from other teleosts, elasmobranch, and tetrapods using in this study. In a non-competitive ELISA system using the MAb, the minimum measurable concentration of chum salmon GH was 20ng/well (approximately 1 pmol).
doi_str_mv 10.2331/fishsci.61.718
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese
subjects Oncorhynchus keta
title Preparation of a Monoclonal Antibody Specific to Chum Salmon Growth Hormone
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