Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause
The culminating phase of ageing in males–andropause is characterized by enhanced activity of the hypothalamic–pituitary–adrenal axis and frequent glucocorticoid excess. In parallel, free testosterone deficiency provides the baseline hormonal milieu for the ageing male. The aim of this study was to i...
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| Veröffentlicht in: | Acta histochemica 2015-07, Vol.117 (6), p.528-535 |
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| creator | Ajdžanović, Vladimir Jarić, Ivana Živanović, Jasmina Filipović, Branko Ristić, Nataša Miler, Marko Milošević, Verica |
| description | The culminating phase of ageing in males–andropause is characterized by enhanced activity of the hypothalamic–pituitary–adrenal axis and frequent glucocorticoid excess. In parallel, free testosterone deficiency provides the baseline hormonal milieu for the ageing male. The aim of this study was to illustrate (using diverse microscopic and biochemical methodologies) the effects of testosterone application on the capacity for adrenocorticotropic hormone (ACTH) and corticosterone secretion in a rat model of the andropause. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5mg/kg b.w./day) was administered for three weeks, while SO and Orx groups received the vehicle alone. ACTH cells and the adrenal cortex were stained using immuno-histochemical, immuno-fluorescent and histochemical procedures. Circulating concentrations of testosterone, estradiol, ACTH and corticosterone, as well as the adrenal tissue corticosterone levels were measured by immunoassays. Testosterone application led to increased (p |
| doi_str_mv | 10.1016/j.acthis.2015.04.002 |
| format | Article |
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In parallel, free testosterone deficiency provides the baseline hormonal milieu for the ageing male. The aim of this study was to illustrate (using diverse microscopic and biochemical methodologies) the effects of testosterone application on the capacity for adrenocorticotropic hormone (ACTH) and corticosterone secretion in a rat model of the andropause. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5mg/kg b.w./day) was administered for three weeks, while SO and Orx groups received the vehicle alone. ACTH cells and the adrenal cortex were stained using immuno-histochemical, immuno-fluorescent and histochemical procedures. Circulating concentrations of testosterone, estradiol, ACTH and corticosterone, as well as the adrenal tissue corticosterone levels were measured by immunoassays. Testosterone application led to increased (p<0.05) serum concentrations of sex steroids. Consequently, in Orx+T rats the ACTH cell nuclei volume increased (p<0.05) by 34%, while the volume density of ACTH cells and their relative intensity of fluorescence decreased (p<0.05) by 46% and 21%, respectively, in comparison with the corresponding parameters in the Orx group. Testosterone also induced vasodilatation in the adrenocortical zona fasciculata, and decreased (p<0.05) the ACTH concentrations and adrenal tissue corticosterone levels by 38% and 31%, respectively, compared to the Orx group. In conclusion, testosterone administration caused a decrease in the capacity for ACTH and corticosterone secretion in a rat model of the andropause.</description><identifier>ISSN: 0065-1281</identifier><identifier>EISSN: 1618-0372</identifier><identifier>DOI: 10.1016/j.acthis.2015.04.002</identifier><identifier>PMID: 25940766</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>ACTH ; Adrenocorticotropic Hormone - blood ; Adrenocorticotropic Hormone - metabolism ; Andropause ; Andropause - physiology ; Animals ; Corticosterone ; Corticosterone - blood ; Corticosterone - metabolism ; Male ; Models, Animal ; Orchiectomy ; Rats ; Testosterone ; Testosterone - pharmacology</subject><ispartof>Acta histochemica, 2015-07, Vol.117 (6), p.528-535</ispartof><rights>2015 Elsevier GmbH</rights><rights>Copyright © 2015 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-7f4c790991eba5cbe7d489a375fab3b99c259156cf0a0b60f40dcc9d0f8be18f3</citedby><cites>FETCH-LOGICAL-c502t-7f4c790991eba5cbe7d489a375fab3b99c259156cf0a0b60f40dcc9d0f8be18f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.acthis.2015.04.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25940766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ajdžanović, Vladimir</creatorcontrib><creatorcontrib>Jarić, Ivana</creatorcontrib><creatorcontrib>Živanović, Jasmina</creatorcontrib><creatorcontrib>Filipović, Branko</creatorcontrib><creatorcontrib>Ristić, Nataša</creatorcontrib><creatorcontrib>Miler, Marko</creatorcontrib><creatorcontrib>Milošević, Verica</creatorcontrib><title>Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>The culminating phase of ageing in males–andropause is characterized by enhanced activity of the hypothalamic–pituitary–adrenal axis and frequent glucocorticoid excess. In parallel, free testosterone deficiency provides the baseline hormonal milieu for the ageing male. The aim of this study was to illustrate (using diverse microscopic and biochemical methodologies) the effects of testosterone application on the capacity for adrenocorticotropic hormone (ACTH) and corticosterone secretion in a rat model of the andropause. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5mg/kg b.w./day) was administered for three weeks, while SO and Orx groups received the vehicle alone. ACTH cells and the adrenal cortex were stained using immuno-histochemical, immuno-fluorescent and histochemical procedures. Circulating concentrations of testosterone, estradiol, ACTH and corticosterone, as well as the adrenal tissue corticosterone levels were measured by immunoassays. Testosterone application led to increased (p<0.05) serum concentrations of sex steroids. Consequently, in Orx+T rats the ACTH cell nuclei volume increased (p<0.05) by 34%, while the volume density of ACTH cells and their relative intensity of fluorescence decreased (p<0.05) by 46% and 21%, respectively, in comparison with the corresponding parameters in the Orx group. Testosterone also induced vasodilatation in the adrenocortical zona fasciculata, and decreased (p<0.05) the ACTH concentrations and adrenal tissue corticosterone levels by 38% and 31%, respectively, compared to the Orx group. In conclusion, testosterone administration caused a decrease in the capacity for ACTH and corticosterone secretion in a rat model of the andropause.</description><subject>ACTH</subject><subject>Adrenocorticotropic Hormone - blood</subject><subject>Adrenocorticotropic Hormone - metabolism</subject><subject>Andropause</subject><subject>Andropause - physiology</subject><subject>Animals</subject><subject>Corticosterone</subject><subject>Corticosterone - blood</subject><subject>Corticosterone - metabolism</subject><subject>Male</subject><subject>Models, Animal</subject><subject>Orchiectomy</subject><subject>Rats</subject><subject>Testosterone</subject><subject>Testosterone - pharmacology</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAURS1UBAPtP6gqL7tJeI7z5U0lNIKChMRmWFvO87PwKBOntgeJf9_MDGXZ1dvcc5_uYey7gFKAaG-2pcH86lNZgWhKqEuA6oytRCv6AmRXfWErgLYpRNWLS3aV0hYAFMjqgl1Wjaqha9sVe9tQyiFlimEibuZ59GiyDxO3hJFMosTzK3E0s0Gf37kLkd-uNw_cTJZjiNnjJ54OyBH2Ezc8msx3wdLIgzuWLEgMs9kn-srOnRkTffu41-zl_m6zfiienn8_rm-fCmygykXnauwUKCVoMA0O1Nm6V0Z2jTODHJTCZYloWnRgYGjB1WARlQXXDyR6J6_Zz1PvHMOf_TJV73xCGkczUdgnLTqoZSOlkku0PkUxhpQiOT1HvzPxXQvQB-N6q0_G9cG4hlovxhfsx8eH_bAj-wn9U7wEfp0CtOx88xR1Qk8TkvWRMGsb_P8__AVtwpYW</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Ajdžanović, Vladimir</creator><creator>Jarić, Ivana</creator><creator>Živanović, Jasmina</creator><creator>Filipović, Branko</creator><creator>Ristić, Nataša</creator><creator>Miler, Marko</creator><creator>Milošević, Verica</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150701</creationdate><title>Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause</title><author>Ajdžanović, Vladimir ; Jarić, Ivana ; Živanović, Jasmina ; Filipović, Branko ; Ristić, Nataša ; Miler, Marko ; Milošević, Verica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-7f4c790991eba5cbe7d489a375fab3b99c259156cf0a0b60f40dcc9d0f8be18f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>ACTH</topic><topic>Adrenocorticotropic Hormone - blood</topic><topic>Adrenocorticotropic Hormone - metabolism</topic><topic>Andropause</topic><topic>Andropause - physiology</topic><topic>Animals</topic><topic>Corticosterone</topic><topic>Corticosterone - blood</topic><topic>Corticosterone - metabolism</topic><topic>Male</topic><topic>Models, Animal</topic><topic>Orchiectomy</topic><topic>Rats</topic><topic>Testosterone</topic><topic>Testosterone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ajdžanović, Vladimir</creatorcontrib><creatorcontrib>Jarić, Ivana</creatorcontrib><creatorcontrib>Živanović, Jasmina</creatorcontrib><creatorcontrib>Filipović, Branko</creatorcontrib><creatorcontrib>Ristić, Nataša</creatorcontrib><creatorcontrib>Miler, Marko</creatorcontrib><creatorcontrib>Milošević, Verica</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ajdžanović, Vladimir</au><au>Jarić, Ivana</au><au>Živanović, Jasmina</au><au>Filipović, Branko</au><au>Ristić, Nataša</au><au>Miler, Marko</au><au>Milošević, Verica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>117</volume><issue>6</issue><spage>528</spage><epage>535</epage><pages>528-535</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>The culminating phase of ageing in males–andropause is characterized by enhanced activity of the hypothalamic–pituitary–adrenal axis and frequent glucocorticoid excess. In parallel, free testosterone deficiency provides the baseline hormonal milieu for the ageing male. The aim of this study was to illustrate (using diverse microscopic and biochemical methodologies) the effects of testosterone application on the capacity for adrenocorticotropic hormone (ACTH) and corticosterone secretion in a rat model of the andropause. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5mg/kg b.w./day) was administered for three weeks, while SO and Orx groups received the vehicle alone. ACTH cells and the adrenal cortex were stained using immuno-histochemical, immuno-fluorescent and histochemical procedures. Circulating concentrations of testosterone, estradiol, ACTH and corticosterone, as well as the adrenal tissue corticosterone levels were measured by immunoassays. Testosterone application led to increased (p<0.05) serum concentrations of sex steroids. Consequently, in Orx+T rats the ACTH cell nuclei volume increased (p<0.05) by 34%, while the volume density of ACTH cells and their relative intensity of fluorescence decreased (p<0.05) by 46% and 21%, respectively, in comparison with the corresponding parameters in the Orx group. Testosterone also induced vasodilatation in the adrenocortical zona fasciculata, and decreased (p<0.05) the ACTH concentrations and adrenal tissue corticosterone levels by 38% and 31%, respectively, compared to the Orx group. In conclusion, testosterone administration caused a decrease in the capacity for ACTH and corticosterone secretion in a rat model of the andropause.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>25940766</pmid><doi>10.1016/j.acthis.2015.04.002</doi><tpages>8</tpages></addata></record> |
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| subjects | ACTH Adrenocorticotropic Hormone - blood Adrenocorticotropic Hormone - metabolism Andropause Andropause - physiology Animals Corticosterone Corticosterone - blood Corticosterone - metabolism Male Models, Animal Orchiectomy Rats Testosterone Testosterone - pharmacology |
| title | Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause |
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