Upregulation of interleukin-1β/transforming growth factor-β1 and hypoxia relate to molecular mechanisms underlying immobilization-induced muscle contracture
ABSTRACT Introduction: In this study we investigated the molecular mechanism underlying muscle contracture in rats. Methods: The rats were divided into immobilization and control groups, and soleus muscles of the right and left sides were selected for analyses. Results: The levels of CD11b and α‐SMA...
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| Veröffentlicht in: | Muscle & nerve 2015-09, Vol.52 (3), p.419-427 |
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| container_title | Muscle & nerve |
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| creator | Honda, Yuichiro Sakamoto, Junya Nakano, Jiro Kataoka, Hideki Sasabe, Ryo Goto, Kyo Tanaka, Miho Origuchi, Tomoki Yoshimura, Toshiro Okita, Minoru |
| description | ABSTRACT
Introduction: In this study we investigated the molecular mechanism underlying muscle contracture in rats. Methods: The rats were divided into immobilization and control groups, and soleus muscles of the right and left sides were selected for analyses. Results: The levels of CD11b and α‐SMA protein, IL‐1β, and TGF‐β1 mRNA, and type I and III collagen protein and mRNA were significantly greater in the immobilization group than in the control group at all time‐points. HIF‐1α mRNA levels were significantly higher in the immobilization group at 4 weeks. Moreover, HIF‐1α, α‐SMA, and type I collagen levels were significantly higher at 4 weeks than at 1 and 2 weeks in the immobilization group. Conclusions: In the early stages of immobilization, upregulation of IL‐1β/TGF‐β1 via macrophages may promote fibroblast differentiation that could affect muscle contracture. The soleus muscle became hypoxic in the later stages of immobilization, suggesting that hypoxia influences the progression of muscle contracture. Muscle Nerve 52:419–427, 2015 |
| doi_str_mv | 10.1002/mus.24558 |
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Introduction: In this study we investigated the molecular mechanism underlying muscle contracture in rats. Methods: The rats were divided into immobilization and control groups, and soleus muscles of the right and left sides were selected for analyses. Results: The levels of CD11b and α‐SMA protein, IL‐1β, and TGF‐β1 mRNA, and type I and III collagen protein and mRNA were significantly greater in the immobilization group than in the control group at all time‐points. HIF‐1α mRNA levels were significantly higher in the immobilization group at 4 weeks. Moreover, HIF‐1α, α‐SMA, and type I collagen levels were significantly higher at 4 weeks than at 1 and 2 weeks in the immobilization group. Conclusions: In the early stages of immobilization, upregulation of IL‐1β/TGF‐β1 via macrophages may promote fibroblast differentiation that could affect muscle contracture. The soleus muscle became hypoxic in the later stages of immobilization, suggesting that hypoxia influences the progression of muscle contracture. Muscle Nerve 52:419–427, 2015</description><identifier>ISSN: 0148-639X</identifier><identifier>EISSN: 1097-4598</identifier><identifier>DOI: 10.1002/mus.24558</identifier><identifier>PMID: 25641164</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Actins - metabolism ; Animals ; CD11b Antigen - metabolism ; Collagen Type I - genetics ; Collagen Type I - metabolism ; Collagen Type III - genetics ; Collagen Type III - metabolism ; Contracture - etiology ; Contracture - metabolism ; fibrosis ; Gene Expression Regulation ; hypoxia ; Hypoxia - genetics ; Hypoxia - metabolism ; Hypoxia-Inducible Factor 1, alpha Subunit - genetics ; IL-1β ; Immobilization - adverse effects ; Interleukin-1beta - genetics ; muscle contracture ; Muscle, Skeletal - metabolism ; Rats ; RNA, Messenger - metabolism ; TGF-β1 ; Transforming Growth Factor beta1 - genetics ; Up-Regulation</subject><ispartof>Muscle & nerve, 2015-09, Vol.52 (3), p.419-427</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3488-ea96fc42855338f30522579a045ceffb963a5959f7a329e5927f179af28fe9603</citedby><cites>FETCH-LOGICAL-c3488-ea96fc42855338f30522579a045ceffb963a5959f7a329e5927f179af28fe9603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmus.24558$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmus.24558$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25641164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Honda, Yuichiro</creatorcontrib><creatorcontrib>Sakamoto, Junya</creatorcontrib><creatorcontrib>Nakano, Jiro</creatorcontrib><creatorcontrib>Kataoka, Hideki</creatorcontrib><creatorcontrib>Sasabe, Ryo</creatorcontrib><creatorcontrib>Goto, Kyo</creatorcontrib><creatorcontrib>Tanaka, Miho</creatorcontrib><creatorcontrib>Origuchi, Tomoki</creatorcontrib><creatorcontrib>Yoshimura, Toshiro</creatorcontrib><creatorcontrib>Okita, Minoru</creatorcontrib><title>Upregulation of interleukin-1β/transforming growth factor-β1 and hypoxia relate to molecular mechanisms underlying immobilization-induced muscle contracture</title><title>Muscle & nerve</title><addtitle>Muscle Nerve</addtitle><description>ABSTRACT
Introduction: In this study we investigated the molecular mechanism underlying muscle contracture in rats. Methods: The rats were divided into immobilization and control groups, and soleus muscles of the right and left sides were selected for analyses. Results: The levels of CD11b and α‐SMA protein, IL‐1β, and TGF‐β1 mRNA, and type I and III collagen protein and mRNA were significantly greater in the immobilization group than in the control group at all time‐points. HIF‐1α mRNA levels were significantly higher in the immobilization group at 4 weeks. Moreover, HIF‐1α, α‐SMA, and type I collagen levels were significantly higher at 4 weeks than at 1 and 2 weeks in the immobilization group. Conclusions: In the early stages of immobilization, upregulation of IL‐1β/TGF‐β1 via macrophages may promote fibroblast differentiation that could affect muscle contracture. The soleus muscle became hypoxic in the later stages of immobilization, suggesting that hypoxia influences the progression of muscle contracture. Muscle Nerve 52:419–427, 2015</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>CD11b Antigen - metabolism</subject><subject>Collagen Type I - genetics</subject><subject>Collagen Type I - metabolism</subject><subject>Collagen Type III - genetics</subject><subject>Collagen Type III - metabolism</subject><subject>Contracture - etiology</subject><subject>Contracture - metabolism</subject><subject>fibrosis</subject><subject>Gene Expression Regulation</subject><subject>hypoxia</subject><subject>Hypoxia - genetics</subject><subject>Hypoxia - metabolism</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</subject><subject>IL-1β</subject><subject>Immobilization - adverse effects</subject><subject>Interleukin-1beta - genetics</subject><subject>muscle contracture</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><subject>TGF-β1</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Up-Regulation</subject><issn>0148-639X</issn><issn>1097-4598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1OGzEQgK2qFYSfQ1-g8rE9LLHX6931sUItrUT_VCJysxzvOHFZ28HeFYSH6UPwIDxTHQLcepk5zDffaGYQekvJCSWknLoxnZQV5-0rNKFENEXFRfsaTQit2qJmYr6PDlL6Qwihbd3sof2S1xWldTVBf2frCMuxV4MNHgeDrR8g9jBeWV_Qh_vpEJVPJkRn_RIvY7gZVtgoPYRYPNxTrHyHV5t1uLUKR8gawEPALvSgszRiB3qlvE0u4dF32bzZeqxzYWF7e_c4trC-GzV0OO-he8A6-DxVD2OEI_TGqD7B8VM-RLPPny5OvxTnP86-nn48LzSr2rYAJWqjq7LlnLHWMMLLkjdCkYprMGYhaqa44MI0ipUCuCgbQ3PdlK0BURN2iN7vvOsYrkdIg3Q2aeh75SGMSdKGsGYbWEY_7FAdQ0oRjFxH61TcSErk9h0yryEf35HZd0_aceGgeyGf75-B6Q64sT1s_m-S32a_n5XFrsOmAW5fOlS8knXDGi4vv5_JOZ3_qn7OL2TN_gFxiamw</recordid><startdate>201509</startdate><enddate>201509</enddate><creator>Honda, Yuichiro</creator><creator>Sakamoto, Junya</creator><creator>Nakano, Jiro</creator><creator>Kataoka, Hideki</creator><creator>Sasabe, Ryo</creator><creator>Goto, Kyo</creator><creator>Tanaka, Miho</creator><creator>Origuchi, Tomoki</creator><creator>Yoshimura, Toshiro</creator><creator>Okita, Minoru</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201509</creationdate><title>Upregulation of interleukin-1β/transforming growth factor-β1 and hypoxia relate to molecular mechanisms underlying immobilization-induced muscle contracture</title><author>Honda, Yuichiro ; Sakamoto, Junya ; Nakano, Jiro ; Kataoka, Hideki ; Sasabe, Ryo ; Goto, Kyo ; Tanaka, Miho ; Origuchi, Tomoki ; Yoshimura, Toshiro ; Okita, Minoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3488-ea96fc42855338f30522579a045ceffb963a5959f7a329e5927f179af28fe9603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>CD11b Antigen - metabolism</topic><topic>Collagen Type I - genetics</topic><topic>Collagen Type I - metabolism</topic><topic>Collagen Type III - genetics</topic><topic>Collagen Type III - metabolism</topic><topic>Contracture - etiology</topic><topic>Contracture - metabolism</topic><topic>fibrosis</topic><topic>Gene Expression Regulation</topic><topic>hypoxia</topic><topic>Hypoxia - genetics</topic><topic>Hypoxia - metabolism</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - genetics</topic><topic>IL-1β</topic><topic>Immobilization - adverse effects</topic><topic>Interleukin-1beta - genetics</topic><topic>muscle contracture</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><topic>TGF-β1</topic><topic>Transforming Growth Factor beta1 - genetics</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Honda, Yuichiro</creatorcontrib><creatorcontrib>Sakamoto, Junya</creatorcontrib><creatorcontrib>Nakano, Jiro</creatorcontrib><creatorcontrib>Kataoka, Hideki</creatorcontrib><creatorcontrib>Sasabe, Ryo</creatorcontrib><creatorcontrib>Goto, Kyo</creatorcontrib><creatorcontrib>Tanaka, Miho</creatorcontrib><creatorcontrib>Origuchi, Tomoki</creatorcontrib><creatorcontrib>Yoshimura, Toshiro</creatorcontrib><creatorcontrib>Okita, Minoru</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Muscle & nerve</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Honda, Yuichiro</au><au>Sakamoto, Junya</au><au>Nakano, Jiro</au><au>Kataoka, Hideki</au><au>Sasabe, Ryo</au><au>Goto, Kyo</au><au>Tanaka, Miho</au><au>Origuchi, Tomoki</au><au>Yoshimura, Toshiro</au><au>Okita, Minoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upregulation of interleukin-1β/transforming growth factor-β1 and hypoxia relate to molecular mechanisms underlying immobilization-induced muscle contracture</atitle><jtitle>Muscle & nerve</jtitle><addtitle>Muscle Nerve</addtitle><date>2015-09</date><risdate>2015</risdate><volume>52</volume><issue>3</issue><spage>419</spage><epage>427</epage><pages>419-427</pages><issn>0148-639X</issn><eissn>1097-4598</eissn><abstract>ABSTRACT
Introduction: In this study we investigated the molecular mechanism underlying muscle contracture in rats. Methods: The rats were divided into immobilization and control groups, and soleus muscles of the right and left sides were selected for analyses. Results: The levels of CD11b and α‐SMA protein, IL‐1β, and TGF‐β1 mRNA, and type I and III collagen protein and mRNA were significantly greater in the immobilization group than in the control group at all time‐points. HIF‐1α mRNA levels were significantly higher in the immobilization group at 4 weeks. Moreover, HIF‐1α, α‐SMA, and type I collagen levels were significantly higher at 4 weeks than at 1 and 2 weeks in the immobilization group. Conclusions: In the early stages of immobilization, upregulation of IL‐1β/TGF‐β1 via macrophages may promote fibroblast differentiation that could affect muscle contracture. The soleus muscle became hypoxic in the later stages of immobilization, suggesting that hypoxia influences the progression of muscle contracture. Muscle Nerve 52:419–427, 2015</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25641164</pmid><doi>10.1002/mus.24558</doi><tpages>9</tpages></addata></record> |
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| ispartof | Muscle & nerve, 2015-09, Vol.52 (3), p.419-427 |
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| subjects | Actins - metabolism Animals CD11b Antigen - metabolism Collagen Type I - genetics Collagen Type I - metabolism Collagen Type III - genetics Collagen Type III - metabolism Contracture - etiology Contracture - metabolism fibrosis Gene Expression Regulation hypoxia Hypoxia - genetics Hypoxia - metabolism Hypoxia-Inducible Factor 1, alpha Subunit - genetics IL-1β Immobilization - adverse effects Interleukin-1beta - genetics muscle contracture Muscle, Skeletal - metabolism Rats RNA, Messenger - metabolism TGF-β1 Transforming Growth Factor beta1 - genetics Up-Regulation |
| title | Upregulation of interleukin-1β/transforming growth factor-β1 and hypoxia relate to molecular mechanisms underlying immobilization-induced muscle contracture |
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