Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications
Although antibiotics have been widely used in clinical applications to treat pathogenic infections at present, the problem of drug-resistance associated with abuse of antibiotics is becoming a potential threat to human beings. We report a biohybrid nanomaterial consisting of antibiotics, enzyme, pol...
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| Veröffentlicht in: | ACS applied materials & interfaces 2015-08, Vol.7 (31), p.17255-17263 |
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| creator | Wu, Yuanhao Long, Yubo Li, Qing-Lan Han, Shuying Ma, Jianbiao Yang, Ying-Wei Gao, Hui |
| description | Although antibiotics have been widely used in clinical applications to treat pathogenic infections at present, the problem of drug-resistance associated with abuse of antibiotics is becoming a potential threat to human beings. We report a biohybrid nanomaterial consisting of antibiotics, enzyme, polymers, hyaluronic acid (HA), and mesoporous silica nanoparticles (MSNs), which exhibits efficient in vitro and in vivo antibacterial activity with good biocompatibility and negligible hemolytic side effect. Herein, biocompatible layer-by-layer (LBL) coated MSNs are designed and crafted to release encapsulated antibiotics, e.g., amoxicillin (AMO), upon triggering with hyaluronidase, produced by various pathogenic Staphylococcus aureus (S. aureus). The LBL coating process comprises lysozyme (Lys), HA, and 1,2-ethanediamine (EDA)-modified polyglycerol methacrylate (PGMA). The Lys and cationic polymers provided multivalent interactions between MSN-Lys-HA-PGMA and bacterial membrane and accordingly immobilized the nanoparticles to facilitate the synergistic effect of these antibacterial agents. Loading process was characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray diffraction spectroscopy (XRD). The minimal inhibition concentration (MIC) of MSN-Lys-HA-PGMA treated to antibiotic resistant bacteria is much lower than that of isodose Lys and AMO. Especially, MSN-Lys-HA-PGMA exhibited good inhibition for pathogens in bacteria-infected wounds in vivo. Therefore, this type of new biohybrid nanomaterials showed great potential as novel antibacterial agents. |
| doi_str_mv | 10.1021/acsami.5b04216 |
| format | Article |
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We report a biohybrid nanomaterial consisting of antibiotics, enzyme, polymers, hyaluronic acid (HA), and mesoporous silica nanoparticles (MSNs), which exhibits efficient in vitro and in vivo antibacterial activity with good biocompatibility and negligible hemolytic side effect. Herein, biocompatible layer-by-layer (LBL) coated MSNs are designed and crafted to release encapsulated antibiotics, e.g., amoxicillin (AMO), upon triggering with hyaluronidase, produced by various pathogenic Staphylococcus aureus (S. aureus). The LBL coating process comprises lysozyme (Lys), HA, and 1,2-ethanediamine (EDA)-modified polyglycerol methacrylate (PGMA). The Lys and cationic polymers provided multivalent interactions between MSN-Lys-HA-PGMA and bacterial membrane and accordingly immobilized the nanoparticles to facilitate the synergistic effect of these antibacterial agents. Loading process was characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray diffraction spectroscopy (XRD). The minimal inhibition concentration (MIC) of MSN-Lys-HA-PGMA treated to antibiotic resistant bacteria is much lower than that of isodose Lys and AMO. Especially, MSN-Lys-HA-PGMA exhibited good inhibition for pathogens in bacteria-infected wounds in vivo. Therefore, this type of new biohybrid nanomaterials showed great potential as novel antibacterial agents.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.5b04216</identifier><identifier>PMID: 26192024</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Amoxicillin - chemistry ; Amoxicillin - pharmacology ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Biocompatible Materials - chemistry ; Cell Line ; Cell Survival - drug effects ; Drug Carriers - chemistry ; Drug Carriers - toxicity ; Dynamic Light Scattering ; Ethylenediamines - chemistry ; Hemolysis - drug effects ; Humans ; Hyaluronic Acid - chemistry ; Microscopy, Fluorescence ; Muramidase - chemistry ; Muramidase - metabolism ; Nanoparticles - chemistry ; Nanostructures - chemistry ; Polymers - chemistry ; Porosity ; Silicon Dioxide - chemistry ; Staphylococcus aureus - drug effects ; Thermogravimetry</subject><ispartof>ACS applied materials & interfaces, 2015-08, Vol.7 (31), p.17255-17263</ispartof><rights>Copyright © American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a330t-ed528cc713e53f69b0a85264fbba50810afd222c77abaa78173d3ab6ca1e79513</citedby><cites>FETCH-LOGICAL-a330t-ed528cc713e53f69b0a85264fbba50810afd222c77abaa78173d3ab6ca1e79513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.5b04216$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.5b04216$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26192024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Yuanhao</creatorcontrib><creatorcontrib>Long, Yubo</creatorcontrib><creatorcontrib>Li, Qing-Lan</creatorcontrib><creatorcontrib>Han, Shuying</creatorcontrib><creatorcontrib>Ma, Jianbiao</creatorcontrib><creatorcontrib>Yang, Ying-Wei</creatorcontrib><creatorcontrib>Gao, Hui</creatorcontrib><title>Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>Although antibiotics have been widely used in clinical applications to treat pathogenic infections at present, the problem of drug-resistance associated with abuse of antibiotics is becoming a potential threat to human beings. We report a biohybrid nanomaterial consisting of antibiotics, enzyme, polymers, hyaluronic acid (HA), and mesoporous silica nanoparticles (MSNs), which exhibits efficient in vitro and in vivo antibacterial activity with good biocompatibility and negligible hemolytic side effect. Herein, biocompatible layer-by-layer (LBL) coated MSNs are designed and crafted to release encapsulated antibiotics, e.g., amoxicillin (AMO), upon triggering with hyaluronidase, produced by various pathogenic Staphylococcus aureus (S. aureus). The LBL coating process comprises lysozyme (Lys), HA, and 1,2-ethanediamine (EDA)-modified polyglycerol methacrylate (PGMA). The Lys and cationic polymers provided multivalent interactions between MSN-Lys-HA-PGMA and bacterial membrane and accordingly immobilized the nanoparticles to facilitate the synergistic effect of these antibacterial agents. Loading process was characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray diffraction spectroscopy (XRD). The minimal inhibition concentration (MIC) of MSN-Lys-HA-PGMA treated to antibiotic resistant bacteria is much lower than that of isodose Lys and AMO. Especially, MSN-Lys-HA-PGMA exhibited good inhibition for pathogens in bacteria-infected wounds in vivo. Therefore, this type of new biohybrid nanomaterials showed great potential as novel antibacterial agents.</description><subject>Amoxicillin - chemistry</subject><subject>Amoxicillin - pharmacology</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biocompatible Materials - chemistry</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - toxicity</subject><subject>Dynamic Light Scattering</subject><subject>Ethylenediamines - chemistry</subject><subject>Hemolysis - drug effects</subject><subject>Humans</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Microscopy, Fluorescence</subject><subject>Muramidase - chemistry</subject><subject>Muramidase - metabolism</subject><subject>Nanoparticles - chemistry</subject><subject>Nanostructures - chemistry</subject><subject>Polymers - chemistry</subject><subject>Porosity</subject><subject>Silicon Dioxide - chemistry</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Thermogravimetry</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EoqWwMqKMBSnFH0mcjG1VPqQIBmCOzo4tXCVxsJMh_55ASjemO-me95XuQeia4BXBlNyD9FCbVSxwRElyguYki6IwpTE9Pe5RNEMX3u8xThjF8Tma0YRkFNNojsocBuVCMYS_S7DMN_lt8KYqHa69V7WoVBlsjP0chDNl8AKNraFTzkDlA21dsNPaSKOaLlg3nREgp2OwbtvKSOiMbfwlOtMjr64Oc4E-Hnbv26cwf3183q7zEBjDXajKmKZScsJUzHSSCQxpTJNICwExTgkGXVJKJecgAHhKOCsZiEQCUTyLCVug5dTbOvvVK98VtfFSVRU0yva-IBwzjglPohFdTah01nundNE6U4MbCoKLH7PFZLY4mB0DN4fuXtSqPOJ_KkfgbgLGYLG3vWvGV_9r-wYwQYNf</recordid><startdate>20150812</startdate><enddate>20150812</enddate><creator>Wu, Yuanhao</creator><creator>Long, Yubo</creator><creator>Li, Qing-Lan</creator><creator>Han, Shuying</creator><creator>Ma, Jianbiao</creator><creator>Yang, Ying-Wei</creator><creator>Gao, Hui</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150812</creationdate><title>Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications</title><author>Wu, Yuanhao ; Long, Yubo ; Li, Qing-Lan ; Han, Shuying ; Ma, Jianbiao ; Yang, Ying-Wei ; Gao, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a330t-ed528cc713e53f69b0a85264fbba50810afd222c77abaa78173d3ab6ca1e79513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amoxicillin - chemistry</topic><topic>Amoxicillin - pharmacology</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biocompatible Materials - chemistry</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Carriers - toxicity</topic><topic>Dynamic Light Scattering</topic><topic>Ethylenediamines - chemistry</topic><topic>Hemolysis - drug effects</topic><topic>Humans</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Microscopy, Fluorescence</topic><topic>Muramidase - chemistry</topic><topic>Muramidase - metabolism</topic><topic>Nanoparticles - chemistry</topic><topic>Nanostructures - chemistry</topic><topic>Polymers - chemistry</topic><topic>Porosity</topic><topic>Silicon Dioxide - chemistry</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Thermogravimetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Yuanhao</creatorcontrib><creatorcontrib>Long, Yubo</creatorcontrib><creatorcontrib>Li, Qing-Lan</creatorcontrib><creatorcontrib>Han, Shuying</creatorcontrib><creatorcontrib>Ma, Jianbiao</creatorcontrib><creatorcontrib>Yang, Ying-Wei</creatorcontrib><creatorcontrib>Gao, Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Yuanhao</au><au>Long, Yubo</au><au>Li, Qing-Lan</au><au>Han, Shuying</au><au>Ma, Jianbiao</au><au>Yang, Ying-Wei</au><au>Gao, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2015-08-12</date><risdate>2015</risdate><volume>7</volume><issue>31</issue><spage>17255</spage><epage>17263</epage><pages>17255-17263</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>Although antibiotics have been widely used in clinical applications to treat pathogenic infections at present, the problem of drug-resistance associated with abuse of antibiotics is becoming a potential threat to human beings. We report a biohybrid nanomaterial consisting of antibiotics, enzyme, polymers, hyaluronic acid (HA), and mesoporous silica nanoparticles (MSNs), which exhibits efficient in vitro and in vivo antibacterial activity with good biocompatibility and negligible hemolytic side effect. Herein, biocompatible layer-by-layer (LBL) coated MSNs are designed and crafted to release encapsulated antibiotics, e.g., amoxicillin (AMO), upon triggering with hyaluronidase, produced by various pathogenic Staphylococcus aureus (S. aureus). The LBL coating process comprises lysozyme (Lys), HA, and 1,2-ethanediamine (EDA)-modified polyglycerol methacrylate (PGMA). The Lys and cationic polymers provided multivalent interactions between MSN-Lys-HA-PGMA and bacterial membrane and accordingly immobilized the nanoparticles to facilitate the synergistic effect of these antibacterial agents. Loading process was characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray diffraction spectroscopy (XRD). The minimal inhibition concentration (MIC) of MSN-Lys-HA-PGMA treated to antibiotic resistant bacteria is much lower than that of isodose Lys and AMO. Especially, MSN-Lys-HA-PGMA exhibited good inhibition for pathogens in bacteria-infected wounds in vivo. Therefore, this type of new biohybrid nanomaterials showed great potential as novel antibacterial agents.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>26192024</pmid><doi>10.1021/acsami.5b04216</doi><tpages>9</tpages></addata></record> |
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| subjects | Amoxicillin - chemistry Amoxicillin - pharmacology Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Biocompatible Materials - chemistry Cell Line Cell Survival - drug effects Drug Carriers - chemistry Drug Carriers - toxicity Dynamic Light Scattering Ethylenediamines - chemistry Hemolysis - drug effects Humans Hyaluronic Acid - chemistry Microscopy, Fluorescence Muramidase - chemistry Muramidase - metabolism Nanoparticles - chemistry Nanostructures - chemistry Polymers - chemistry Porosity Silicon Dioxide - chemistry Staphylococcus aureus - drug effects Thermogravimetry |
| title | Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications |
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