Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis)

This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IH...

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Veröffentlicht in:Journal of comparative pathology 2015-08, Vol.153 (2-3), p.190-195
Hauptverfasser: Sirri, R., Bianco, C., Beraldo, P., Mandrioli, L., Pulvirenti, I., Brachelente, C., Galeotti, M., Sarli, G.
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container_end_page 195
container_issue 2-3
container_start_page 190
container_title Journal of comparative pathology
container_volume 153
creator Sirri, R.
Bianco, C.
Beraldo, P.
Mandrioli, L.
Pulvirenti, I.
Brachelente, C.
Galeotti, M.
Sarli, G.
description This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IHC was investigated in silico using the Protein Blast system. Microscopically, the lesion appeared as a ‘small round cell’ undifferentiated sarcoma with rare myotube formation. IHC identified expression of sarcomeric actin and vimentin and these molecules showed the highest protein sequence identity. Lower protein sequence identity coincided with negative immunolabelling for desmin, MyoD1, myogenin and CD3. TEM revealed myofibrils, but without a defined sarcomeric architecture. The diagnosis of solid alveolar rhabdomyosarcoma of soft tissues was achieved on the basis of histological and ultrastructural findings.
doi_str_mv 10.1016/j.jcpa.2015.05.001
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subjects Animals
Biomarkers, Tumor - analysis
Fish Diseases - pathology
Immunohistochemistry
Microscopy, Electron, Transmission
protein blast
rhabdomyosarcoma
Rhabdomyosarcoma - pathology
Rhabdomyosarcoma - veterinary
salmonid
Soft Tissue Neoplasms - pathology
Soft Tissue Neoplasms - veterinary
Trout
title Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis)
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