Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis)
This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IH...
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Veröffentlicht in: | Journal of comparative pathology 2015-08, Vol.153 (2-3), p.190-195 |
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container_title | Journal of comparative pathology |
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creator | Sirri, R. Bianco, C. Beraldo, P. Mandrioli, L. Pulvirenti, I. Brachelente, C. Galeotti, M. Sarli, G. |
description | This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IHC was investigated in silico using the Protein Blast system. Microscopically, the lesion appeared as a ‘small round cell’ undifferentiated sarcoma with rare myotube formation. IHC identified expression of sarcomeric actin and vimentin and these molecules showed the highest protein sequence identity. Lower protein sequence identity coincided with negative immunolabelling for desmin, MyoD1, myogenin and CD3. TEM revealed myofibrils, but without a defined sarcomeric architecture. The diagnosis of solid alveolar rhabdomyosarcoma of soft tissues was achieved on the basis of histological and ultrastructural findings. |
doi_str_mv | 10.1016/j.jcpa.2015.05.001 |
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The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IHC was investigated in silico using the Protein Blast system. Microscopically, the lesion appeared as a ‘small round cell’ undifferentiated sarcoma with rare myotube formation. IHC identified expression of sarcomeric actin and vimentin and these molecules showed the highest protein sequence identity. Lower protein sequence identity coincided with negative immunolabelling for desmin, MyoD1, myogenin and CD3. TEM revealed myofibrils, but without a defined sarcomeric architecture. The diagnosis of solid alveolar rhabdomyosarcoma of soft tissues was achieved on the basis of histological and ultrastructural findings.</description><identifier>ISSN: 0021-9975</identifier><identifier>EISSN: 1532-3129</identifier><identifier>DOI: 10.1016/j.jcpa.2015.05.001</identifier><identifier>PMID: 26072379</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Biomarkers, Tumor - analysis ; Fish Diseases - pathology ; Immunohistochemistry ; Microscopy, Electron, Transmission ; protein blast ; rhabdomyosarcoma ; Rhabdomyosarcoma - pathology ; Rhabdomyosarcoma - veterinary ; salmonid ; Soft Tissue Neoplasms - pathology ; Soft Tissue Neoplasms - veterinary ; Trout</subject><ispartof>Journal of comparative pathology, 2015-08, Vol.153 (2-3), p.190-195</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-ed0b493dee26c129d59a6cac4003b6b1fce93bcd76126cecf188b97d7ef6d1203</citedby><cites>FETCH-LOGICAL-c426t-ed0b493dee26c129d59a6cac4003b6b1fce93bcd76126cecf188b97d7ef6d1203</cites><orcidid>0000-0002-8603-8418</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcpa.2015.05.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26072379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sirri, R.</creatorcontrib><creatorcontrib>Bianco, C.</creatorcontrib><creatorcontrib>Beraldo, P.</creatorcontrib><creatorcontrib>Mandrioli, L.</creatorcontrib><creatorcontrib>Pulvirenti, I.</creatorcontrib><creatorcontrib>Brachelente, C.</creatorcontrib><creatorcontrib>Galeotti, M.</creatorcontrib><creatorcontrib>Sarli, G.</creatorcontrib><title>Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis)</title><title>Journal of comparative pathology</title><addtitle>J Comp Pathol</addtitle><description>This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IHC was investigated in silico using the Protein Blast system. Microscopically, the lesion appeared as a ‘small round cell’ undifferentiated sarcoma with rare myotube formation. IHC identified expression of sarcomeric actin and vimentin and these molecules showed the highest protein sequence identity. Lower protein sequence identity coincided with negative immunolabelling for desmin, MyoD1, myogenin and CD3. TEM revealed myofibrils, but without a defined sarcomeric architecture. The diagnosis of solid alveolar rhabdomyosarcoma of soft tissues was achieved on the basis of histological and ultrastructural findings.</description><subject>Animals</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Fish Diseases - pathology</subject><subject>Immunohistochemistry</subject><subject>Microscopy, Electron, Transmission</subject><subject>protein blast</subject><subject>rhabdomyosarcoma</subject><subject>Rhabdomyosarcoma - pathology</subject><subject>Rhabdomyosarcoma - veterinary</subject><subject>salmonid</subject><subject>Soft Tissue Neoplasms - pathology</subject><subject>Soft Tissue Neoplasms - veterinary</subject><subject>Trout</subject><issn>0021-9975</issn><issn>1532-3129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kN1LwzAUxYMoOj_-AR8kj_Oh8yZZ2wV8mcMvEBQ3n0Oa3GJm28ykFfbf2zL1UThwX37ncO4h5JzBhAHLrtaTtdnoCQeWTqAXsD0yYqngiWBc7pMRAGeJlHl6RI5jXAPAbJbyQ3LEM8i5yOWIvLy-68L6euujDsbXmvqSLn3Z0pWLscNIXUN1Q-e2q1p6E7z_oKvgu5aOl7r6wso1XaSlb1rX6MrFy1NyUOoq4tnPPSFvd7erxUPy9Hz_uJg_JWbKszZBC8VUCovIM9O3tanUmdFmCiCKrGClQSkKY_OM9QCaks1mhcxtjmVmGQdxQsa73E3wn33PVtUuGqwq3aDvomI5CD4VTA4o36Em-BgDlmoTXK3DVjFQw5JqrYYl1bCkgl7AetPFT35X1Gj_LL_T9cD1DsD-yy-HQUXjsDFoXUDTKuvdf_nfsQCE-Q</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Sirri, R.</creator><creator>Bianco, C.</creator><creator>Beraldo, P.</creator><creator>Mandrioli, L.</creator><creator>Pulvirenti, I.</creator><creator>Brachelente, C.</creator><creator>Galeotti, M.</creator><creator>Sarli, G.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8603-8418</orcidid></search><sort><creationdate>20150801</creationdate><title>Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis)</title><author>Sirri, R. ; Bianco, C. ; Beraldo, P. ; Mandrioli, L. ; Pulvirenti, I. ; Brachelente, C. ; Galeotti, M. ; Sarli, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-ed0b493dee26c129d59a6cac4003b6b1fce93bcd76126cecf188b97d7ef6d1203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Fish Diseases - pathology</topic><topic>Immunohistochemistry</topic><topic>Microscopy, Electron, Transmission</topic><topic>protein blast</topic><topic>rhabdomyosarcoma</topic><topic>Rhabdomyosarcoma - pathology</topic><topic>Rhabdomyosarcoma - veterinary</topic><topic>salmonid</topic><topic>Soft Tissue Neoplasms - pathology</topic><topic>Soft Tissue Neoplasms - veterinary</topic><topic>Trout</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sirri, R.</creatorcontrib><creatorcontrib>Bianco, C.</creatorcontrib><creatorcontrib>Beraldo, P.</creatorcontrib><creatorcontrib>Mandrioli, L.</creatorcontrib><creatorcontrib>Pulvirenti, I.</creatorcontrib><creatorcontrib>Brachelente, C.</creatorcontrib><creatorcontrib>Galeotti, M.</creatorcontrib><creatorcontrib>Sarli, G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of comparative pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sirri, R.</au><au>Bianco, C.</au><au>Beraldo, P.</au><au>Mandrioli, L.</au><au>Pulvirenti, I.</au><au>Brachelente, C.</au><au>Galeotti, M.</au><au>Sarli, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis)</atitle><jtitle>Journal of comparative pathology</jtitle><addtitle>J Comp Pathol</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>153</volume><issue>2-3</issue><spage>190</spage><epage>195</epage><pages>190-195</pages><issn>0021-9975</issn><eissn>1532-3129</eissn><abstract>This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IHC was investigated in silico using the Protein Blast system. Microscopically, the lesion appeared as a ‘small round cell’ undifferentiated sarcoma with rare myotube formation. IHC identified expression of sarcomeric actin and vimentin and these molecules showed the highest protein sequence identity. Lower protein sequence identity coincided with negative immunolabelling for desmin, MyoD1, myogenin and CD3. TEM revealed myofibrils, but without a defined sarcomeric architecture. The diagnosis of solid alveolar rhabdomyosarcoma of soft tissues was achieved on the basis of histological and ultrastructural findings.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26072379</pmid><doi>10.1016/j.jcpa.2015.05.001</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-8603-8418</orcidid></addata></record> |
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subjects | Animals Biomarkers, Tumor - analysis Fish Diseases - pathology Immunohistochemistry Microscopy, Electron, Transmission protein blast rhabdomyosarcoma Rhabdomyosarcoma - pathology Rhabdomyosarcoma - veterinary salmonid Soft Tissue Neoplasms - pathology Soft Tissue Neoplasms - veterinary Trout |
title | Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis) |
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