Folding and Functional Complementation of Engineered Fragments from Yeast Phosphoglycerate Kinase

A set of protein fragments was produced by site-directed mutagenesis followed by chemical cleavage of phosphoglycerate kinase according to a previously described method [Pecorari et al. (1993) Protein Eng. 6, 313−325]. The cleavage positions were chosen in order to correspond to limits between struc...

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Veröffentlicht in:	Biochemistry (Easton) 1996-03, Vol.35 (11), p.3465-3476
Hauptverfasser:	Pecorari, Frédéric, Guilbert, Christophe, Minard, Philippe, Desmadril, Michel, Yon, Jeannine M
Format:	Artikel
Sprache:	eng
Schlagworte:	Circular Dichroism Cross-Linking Reagents - chemistry Fungal Proteins - chemistry Molecular Weight Phosphoglycerate Kinase - chemistry Protein Denaturation Protein Structure, Secondary Protein Structure, Tertiary Saccharomyces cerevisiae Structure-Activity Relationship Temperature Thermodynamics Yeasts - enzymology
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