Dermal expression of laminin-332 and type IV collagen in humans with severe sepsis
Background An intact basement membrane at the dermal–epidermal junction is essential to the viability of the skin. The effect of sepsis on the basement membrane is unknown. Methods Skin biopsies were used to study basement membrane structure in severe sepsis (Day 1). Subsequent biopsies were taken o...
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| Veröffentlicht in: | Acta anaesthesiologica Scandinavica 2015-09, Vol.59 (8), p.1009-1014 |
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| container_title | Acta anaesthesiologica Scandinavica |
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| creator | Koskela, M. Gäddnäs, F. Koivukangas, V. Oikarinen, A. Laurila, J. Kallioinen, M. Ala-Kokko, T. I. |
| description | Background
An intact basement membrane at the dermal–epidermal junction is essential to the viability of the skin. The effect of sepsis on the basement membrane is unknown.
Methods
Skin biopsies were used to study basement membrane structure in severe sepsis (Day 1). Subsequent biopsies were taken on Day 8 and at 3 months in the survivors. Immunohistochemical staining was undertaken using laminin‐223 and type IV collagen. Twenty patients with severe sepsis and four control subjects were included in the analysis.
Results
Intensive care unit mortality was 4/20, and total 30‐day mortality was 5/20. Exactly, 7/17 of patients with severe sepsis exhibited weak or absent laminin‐332 expression and 11/15 exhibited weak or absent type IV collagen expression compared with 0/4 of control subjects on Day 1 in intact skin. The proportion of sepsis patients with weak or absent laminin‐332 expression was 5/11 on Day 8 and fell to 1/7 at 3 months. The proportion of sepsis patients with weak or absent type IV collagen expression was 10/11 on Day 8 and 4/7 at 3 months.
Conclusion
These findings suggest that basement membrane formation may be compromised in patients with severe sepsis. |
| doi_str_mv | 10.1111/aas.12539 |
| format | Article |
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An intact basement membrane at the dermal–epidermal junction is essential to the viability of the skin. The effect of sepsis on the basement membrane is unknown.
Methods
Skin biopsies were used to study basement membrane structure in severe sepsis (Day 1). Subsequent biopsies were taken on Day 8 and at 3 months in the survivors. Immunohistochemical staining was undertaken using laminin‐223 and type IV collagen. Twenty patients with severe sepsis and four control subjects were included in the analysis.
Results
Intensive care unit mortality was 4/20, and total 30‐day mortality was 5/20. Exactly, 7/17 of patients with severe sepsis exhibited weak or absent laminin‐332 expression and 11/15 exhibited weak or absent type IV collagen expression compared with 0/4 of control subjects on Day 1 in intact skin. The proportion of sepsis patients with weak or absent laminin‐332 expression was 5/11 on Day 8 and fell to 1/7 at 3 months. The proportion of sepsis patients with weak or absent type IV collagen expression was 10/11 on Day 8 and 4/7 at 3 months.
Conclusion
These findings suggest that basement membrane formation may be compromised in patients with severe sepsis.</description><identifier>ISSN: 0001-5172</identifier><identifier>EISSN: 1399-6576</identifier><identifier>DOI: 10.1111/aas.12539</identifier><identifier>PMID: 26032240</identifier><identifier>CODEN: AANEAB</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Basement Membrane - metabolism ; Collagen Type IV - metabolism ; Female ; Follow-Up Studies ; Humans ; Immunohistochemistry ; Laminin - metabolism ; Male ; Middle Aged ; Sepsis - metabolism ; Skin - metabolism</subject><ispartof>Acta anaesthesiologica Scandinavica, 2015-09, Vol.59 (8), p.1009-1014</ispartof><rights>2015 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd</rights><rights>2015 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 The Acta Anaesthesiologica Scandinavica Foundation</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3919-d9e5e34c6a42b6529b7622f4136f228943de1af051aef2590fd9d661718421e53</citedby><cites>FETCH-LOGICAL-c3919-d9e5e34c6a42b6529b7622f4136f228943de1af051aef2590fd9d661718421e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faas.12539$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faas.12539$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26032240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koskela, M.</creatorcontrib><creatorcontrib>Gäddnäs, F.</creatorcontrib><creatorcontrib>Koivukangas, V.</creatorcontrib><creatorcontrib>Oikarinen, A.</creatorcontrib><creatorcontrib>Laurila, J.</creatorcontrib><creatorcontrib>Kallioinen, M.</creatorcontrib><creatorcontrib>Ala-Kokko, T. I.</creatorcontrib><title>Dermal expression of laminin-332 and type IV collagen in humans with severe sepsis</title><title>Acta anaesthesiologica Scandinavica</title><addtitle>Acta Anaesthesiol. Scand</addtitle><description>Background
An intact basement membrane at the dermal–epidermal junction is essential to the viability of the skin. The effect of sepsis on the basement membrane is unknown.
Methods
Skin biopsies were used to study basement membrane structure in severe sepsis (Day 1). Subsequent biopsies were taken on Day 8 and at 3 months in the survivors. Immunohistochemical staining was undertaken using laminin‐223 and type IV collagen. Twenty patients with severe sepsis and four control subjects were included in the analysis.
Results
Intensive care unit mortality was 4/20, and total 30‐day mortality was 5/20. Exactly, 7/17 of patients with severe sepsis exhibited weak or absent laminin‐332 expression and 11/15 exhibited weak or absent type IV collagen expression compared with 0/4 of control subjects on Day 1 in intact skin. The proportion of sepsis patients with weak or absent laminin‐332 expression was 5/11 on Day 8 and fell to 1/7 at 3 months. The proportion of sepsis patients with weak or absent type IV collagen expression was 10/11 on Day 8 and 4/7 at 3 months.
Conclusion
These findings suggest that basement membrane formation may be compromised in patients with severe sepsis.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Basement Membrane - metabolism</subject><subject>Collagen Type IV - metabolism</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Laminin - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Sepsis - metabolism</subject><subject>Skin - metabolism</subject><issn>0001-5172</issn><issn>1399-6576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1P3DAQxa2KqizbHvgHKktc4BDw2LGzPq6WzwpBPyhIXCxvMimGxAn2Btj_vqYLHJA6l6eRfu9p5hGyCWwX0uxZG3eBS6E_kBEIrTMlC7VGRowxyCQUfJ1sxHibVpFr_Ymsc8UE5zkbkZ_7GFrbUHzqA8boOk-7mja2dd75TAhOra_oYtkjPbmkZdc09g966jy9GVrrI310ixsa8QEDJumji5_Jx9o2Eb-86Jj8Pjy4mB1np-dHJ7PpaVYKDTqrNEoUealszudKcj0vFOd1DkLVnE90LioEWzMJFmsuNasrXSkFBUxyDijFmGyvcvvQ3Q8YF6Z1scR0ocduiAYKxpUUMv06Jlvv0NtuCD5d90yBlnLCdaJ2VlQZuhgD1qYPrrVhaYCZ56JNKtr8KzqxX18Sh3mL1Rv52mwC9lbAo2tw-f8kM53-eo3MVg4XF_j05rDhzqhCFNJcnR0ZDRf71_m3H-a7-AvZ6pOD</recordid><startdate>201509</startdate><enddate>201509</enddate><creator>Koskela, M.</creator><creator>Gäddnäs, F.</creator><creator>Koivukangas, V.</creator><creator>Oikarinen, A.</creator><creator>Laurila, J.</creator><creator>Kallioinen, M.</creator><creator>Ala-Kokko, T. I.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>201509</creationdate><title>Dermal expression of laminin-332 and type IV collagen in humans with severe sepsis</title><author>Koskela, M. ; Gäddnäs, F. ; Koivukangas, V. ; Oikarinen, A. ; Laurila, J. ; Kallioinen, M. ; Ala-Kokko, T. I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3919-d9e5e34c6a42b6529b7622f4136f228943de1af051aef2590fd9d661718421e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Basement Membrane - metabolism</topic><topic>Collagen Type IV - metabolism</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Laminin - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Sepsis - metabolism</topic><topic>Skin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koskela, M.</creatorcontrib><creatorcontrib>Gäddnäs, F.</creatorcontrib><creatorcontrib>Koivukangas, V.</creatorcontrib><creatorcontrib>Oikarinen, A.</creatorcontrib><creatorcontrib>Laurila, J.</creatorcontrib><creatorcontrib>Kallioinen, M.</creatorcontrib><creatorcontrib>Ala-Kokko, T. I.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta anaesthesiologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koskela, M.</au><au>Gäddnäs, F.</au><au>Koivukangas, V.</au><au>Oikarinen, A.</au><au>Laurila, J.</au><au>Kallioinen, M.</au><au>Ala-Kokko, T. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dermal expression of laminin-332 and type IV collagen in humans with severe sepsis</atitle><jtitle>Acta anaesthesiologica Scandinavica</jtitle><addtitle>Acta Anaesthesiol. Scand</addtitle><date>2015-09</date><risdate>2015</risdate><volume>59</volume><issue>8</issue><spage>1009</spage><epage>1014</epage><pages>1009-1014</pages><issn>0001-5172</issn><eissn>1399-6576</eissn><coden>AANEAB</coden><abstract>Background
An intact basement membrane at the dermal–epidermal junction is essential to the viability of the skin. The effect of sepsis on the basement membrane is unknown.
Methods
Skin biopsies were used to study basement membrane structure in severe sepsis (Day 1). Subsequent biopsies were taken on Day 8 and at 3 months in the survivors. Immunohistochemical staining was undertaken using laminin‐223 and type IV collagen. Twenty patients with severe sepsis and four control subjects were included in the analysis.
Results
Intensive care unit mortality was 4/20, and total 30‐day mortality was 5/20. Exactly, 7/17 of patients with severe sepsis exhibited weak or absent laminin‐332 expression and 11/15 exhibited weak or absent type IV collagen expression compared with 0/4 of control subjects on Day 1 in intact skin. The proportion of sepsis patients with weak or absent laminin‐332 expression was 5/11 on Day 8 and fell to 1/7 at 3 months. The proportion of sepsis patients with weak or absent type IV collagen expression was 10/11 on Day 8 and 4/7 at 3 months.
Conclusion
These findings suggest that basement membrane formation may be compromised in patients with severe sepsis.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26032240</pmid><doi>10.1111/aas.12539</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Basement Membrane - metabolism Collagen Type IV - metabolism Female Follow-Up Studies Humans Immunohistochemistry Laminin - metabolism Male Middle Aged Sepsis - metabolism Skin - metabolism |
| title | Dermal expression of laminin-332 and type IV collagen in humans with severe sepsis |
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