Screening for restriction endonucleases in aerobic, thermophilic eubacteria
A total of 216 Icelandic aerobic, heterotrophic, thermophiles belonging to three different genera were screened for type II restriction endonucleases. The frequency of positive strains was 44% for both Thermus and Bacillus but 63% for Rhodothermus. Approximately half of the enzymes from each group w...
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| Veröffentlicht in: | Biotechnology techniques 1996-01, Vol.10 (1), p.13-18 |
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| container_title | Biotechnology techniques |
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| creator | Hj rleifsd ttir, Sigr dur P tursd ttir, S lveig K. Korpela, Jaana Torsti, Anna-Maria Mattila, Pekka Kristj nsson, Jakob K. |
| description | A total of 216 Icelandic aerobic, heterotrophic, thermophiles belonging to three different genera were screened for type II restriction endonucleases. The frequency of positive strains was 44% for both Thermus and Bacillus but 63% for Rhodothermus. Approximately half of the enzymes from each group were characterized and a total of 14 different restriction enzymes were found. In all cases they were isoschizomers of known enzymes. Thermus contained 9 different types, Bacillus 6 and Rhodothermus had 3. This is the first time that isoschizomers of BspEI, BgII, EagI and EcoRV are found in Thermus and BstBI and EcoRV are found in Rhodothermus. |
| doi_str_mv | 10.1007/BF00161077 |
| format | Article |
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| identifier | ISSN: 0951-208X |
| ispartof | Biotechnology techniques, 1996-01, Vol.10 (1), p.13-18 |
| issn | 0951-208X 1573-6784 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17024179 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Bacillus Rhodothermus Thermus |
| title | Screening for restriction endonucleases in aerobic, thermophilic eubacteria |
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