Nitric oxide synthase is an active enzyme in the spiral ganglion cells of the rat cochlea
Nitric oxide (NO) mediates the effects of the excitatory amino acids in the central nervous system. Excitatory amino acids, in particular l-glutamate, are thought to be the neurotransmitter(s) present at the cochlear hair cell-afferent nerve synapse. To our knowledge, no studies to date have documen...
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| Veröffentlicht in: | Hearing research 1994-09, Vol.79 (1), p.39-47 |
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| creator | Zdanski, Carlton J. Prazma, Jiri Petrusz, Peter Grossman, Gail Raynor, Eileen Smith, Timothy L. Pillsbury, Harold C. |
| description | Nitric oxide (NO) mediates the effects of the excitatory amino acids in the central nervous system. Excitatory amino acids, in particular
l-glutamate, are thought to be the neurotransmitter(s) present at the cochlear hair cell-afferent nerve synapse. To our knowledge, no studies to date have documented the presence of NO in the cochlea nor attempted to elucidate the role of NO in hearing. Rat cochlea frozen sections were examined for the presence of nitric oxide synthase (NOS) by NADPH diaphorase histochemistry. Vibratome sections of rat cochlea were examined by immunocytochemistry with an antibody to citrulline, an indication of NOS activity. Spiral ganglion cells in the rat cochlea were positive by NADPH diaphorase histochemistry and by anti-citrulline immunocytochemistry. These results indicate that NOS is present and that the enzyme actively produces nitric oxide in the spiral ganglion cells of the rat cochlea. Given our current understanding of neurotransmission in the cochlea, it is reasonable to postulate that the actions of NO in cochlear neuronal tissue are similar to the actions of NO in the CNS and that NO acts as a neurotransmitter/neuromodulator in the cochlea. In addition, because NO has been implicated as a mediator of excitotoxicity in the CNS, NO may play a role in neurotoxicity in the cochlea. |
| doi_str_mv | 10.1016/0378-5955(94)90125-2 |
| format | Article |
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l-glutamate, are thought to be the neurotransmitter(s) present at the cochlear hair cell-afferent nerve synapse. To our knowledge, no studies to date have documented the presence of NO in the cochlea nor attempted to elucidate the role of NO in hearing. Rat cochlea frozen sections were examined for the presence of nitric oxide synthase (NOS) by NADPH diaphorase histochemistry. Vibratome sections of rat cochlea were examined by immunocytochemistry with an antibody to citrulline, an indication of NOS activity. Spiral ganglion cells in the rat cochlea were positive by NADPH diaphorase histochemistry and by anti-citrulline immunocytochemistry. These results indicate that NOS is present and that the enzyme actively produces nitric oxide in the spiral ganglion cells of the rat cochlea. Given our current understanding of neurotransmission in the cochlea, it is reasonable to postulate that the actions of NO in cochlear neuronal tissue are similar to the actions of NO in the CNS and that NO acts as a neurotransmitter/neuromodulator in the cochlea. In addition, because NO has been implicated as a mediator of excitotoxicity in the CNS, NO may play a role in neurotoxicity in the cochlea.</description><identifier>ISSN: 0378-5955</identifier><identifier>EISSN: 1878-5891</identifier><identifier>DOI: 10.1016/0378-5955(94)90125-2</identifier><identifier>PMID: 7528738</identifier><identifier>CODEN: HERED3</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Oxidoreductases - metabolism ; Animals ; Antibodies, Monoclonal - immunology ; Biological and medical sciences ; Citrulline ; Citrulline - analysis ; Citrulline - immunology ; Cochlea - enzymology ; Cochlea - innervation ; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation ; Frozen Sections ; Fundamental and applied biological sciences. Psychology ; Hair Cells, Auditory - cytology ; Hair Cells, Auditory - physiology ; Immunohistochemistry ; In Vitro Techniques ; Male ; NADPH Dehydrogenase - chemistry ; Neuronal NADPH diaphorase ; Neurotoxicity ; Neurotransmission ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase ; Prosencephalon - cytology ; Prosencephalon - enzymology ; Rats ; Rats, Sprague-Dawley ; Spiral Ganglion - cytology ; Spiral Ganglion - enzymology ; Spiral ganglion cells ; Synaptic Transmission - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Hearing research, 1994-09, Vol.79 (1), p.39-47</ispartof><rights>1994</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-ee83b558b5236bb723fe7b867e069519a5ab78a9848b0901387ce9ba89a9b66c3</citedby><cites>FETCH-LOGICAL-c417t-ee83b558b5236bb723fe7b867e069519a5ab78a9848b0901387ce9ba89a9b66c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-5955(94)90125-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3310747$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7528738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zdanski, Carlton J.</creatorcontrib><creatorcontrib>Prazma, Jiri</creatorcontrib><creatorcontrib>Petrusz, Peter</creatorcontrib><creatorcontrib>Grossman, Gail</creatorcontrib><creatorcontrib>Raynor, Eileen</creatorcontrib><creatorcontrib>Smith, Timothy L.</creatorcontrib><creatorcontrib>Pillsbury, Harold C.</creatorcontrib><title>Nitric oxide synthase is an active enzyme in the spiral ganglion cells of the rat cochlea</title><title>Hearing research</title><addtitle>Hear Res</addtitle><description>Nitric oxide (NO) mediates the effects of the excitatory amino acids in the central nervous system. Excitatory amino acids, in particular
l-glutamate, are thought to be the neurotransmitter(s) present at the cochlear hair cell-afferent nerve synapse. To our knowledge, no studies to date have documented the presence of NO in the cochlea nor attempted to elucidate the role of NO in hearing. Rat cochlea frozen sections were examined for the presence of nitric oxide synthase (NOS) by NADPH diaphorase histochemistry. Vibratome sections of rat cochlea were examined by immunocytochemistry with an antibody to citrulline, an indication of NOS activity. Spiral ganglion cells in the rat cochlea were positive by NADPH diaphorase histochemistry and by anti-citrulline immunocytochemistry. These results indicate that NOS is present and that the enzyme actively produces nitric oxide in the spiral ganglion cells of the rat cochlea. Given our current understanding of neurotransmission in the cochlea, it is reasonable to postulate that the actions of NO in cochlear neuronal tissue are similar to the actions of NO in the CNS and that NO acts as a neurotransmitter/neuromodulator in the cochlea. In addition, because NO has been implicated as a mediator of excitotoxicity in the CNS, NO may play a role in neurotoxicity in the cochlea.</description><subject>Amino Acid Oxidoreductases - metabolism</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Citrulline</subject><subject>Citrulline - analysis</subject><subject>Citrulline - immunology</subject><subject>Cochlea - enzymology</subject><subject>Cochlea - innervation</subject><subject>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</subject><subject>Frozen Sections</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hair Cells, Auditory - cytology</subject><subject>Hair Cells, Auditory - physiology</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>NADPH Dehydrogenase - chemistry</subject><subject>Neuronal NADPH diaphorase</subject><subject>Neurotoxicity</subject><subject>Neurotransmission</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase</subject><subject>Prosencephalon - cytology</subject><subject>Prosencephalon - enzymology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spiral Ganglion - cytology</subject><subject>Spiral Ganglion - enzymology</subject><subject>Spiral ganglion cells</subject><subject>Synaptic Transmission - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0378-5955</issn><issn>1878-5891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PGzEQhi3UiqaUf0AlHyrUHrb4Y722L0gIAa2EyoUeOFljZ5YYbbzB3iDSX88uiXLsaaR5nxnNPISccPaTM96cMalNpaxS3239wzIuVCUOyIybqW0s_0Bme-QT-VzKE2NcyVockkOthNHSzMjDnzjkGGj_GudIyyYNCyhIY6GQKIQhviDF9G-zHHuJDouRWcUMHX2E9NjFPtGAXVdo376HGQYa-rDoEL6Qjy10BY939Yj8vb66v_xV3d7d_L68uK1CzfVQIRrplTJeCdl4r4VsUXvTaGSNVdyCAq8NWFMbz8YvpdEBrQdjwfqmCfKInG73rnL_vMYyuGUs01GQsF8XxzUTohZqBOstGHJfSsbWrXJcQt44ztxk1E263KTL2dq9G3ViHPu627_2S5zvh3YKx_zbLocSoGszpBDLHpOSM13rETvfYji6eImYXQkRU8B5zBgGN-_j_-94A2L3kV0</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Zdanski, Carlton J.</creator><creator>Prazma, Jiri</creator><creator>Petrusz, Peter</creator><creator>Grossman, Gail</creator><creator>Raynor, Eileen</creator><creator>Smith, Timothy L.</creator><creator>Pillsbury, Harold C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19940901</creationdate><title>Nitric oxide synthase is an active enzyme in the spiral ganglion cells of the rat cochlea</title><author>Zdanski, Carlton J. ; Prazma, Jiri ; Petrusz, Peter ; Grossman, Gail ; Raynor, Eileen ; Smith, Timothy L. ; Pillsbury, Harold C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-ee83b558b5236bb723fe7b867e069519a5ab78a9848b0901387ce9ba89a9b66c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Oxidoreductases - metabolism</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biological and medical sciences</topic><topic>Citrulline</topic><topic>Citrulline - analysis</topic><topic>Citrulline - immunology</topic><topic>Cochlea - enzymology</topic><topic>Cochlea - innervation</topic><topic>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</topic><topic>Frozen Sections</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hair Cells, Auditory - cytology</topic><topic>Hair Cells, Auditory - physiology</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>NADPH Dehydrogenase - chemistry</topic><topic>Neuronal NADPH diaphorase</topic><topic>Neurotoxicity</topic><topic>Neurotransmission</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase</topic><topic>Prosencephalon - cytology</topic><topic>Prosencephalon - enzymology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spiral Ganglion - cytology</topic><topic>Spiral Ganglion - enzymology</topic><topic>Spiral ganglion cells</topic><topic>Synaptic Transmission - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zdanski, Carlton J.</creatorcontrib><creatorcontrib>Prazma, Jiri</creatorcontrib><creatorcontrib>Petrusz, Peter</creatorcontrib><creatorcontrib>Grossman, Gail</creatorcontrib><creatorcontrib>Raynor, Eileen</creatorcontrib><creatorcontrib>Smith, Timothy L.</creatorcontrib><creatorcontrib>Pillsbury, Harold C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Hearing research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zdanski, Carlton J.</au><au>Prazma, Jiri</au><au>Petrusz, Peter</au><au>Grossman, Gail</au><au>Raynor, Eileen</au><au>Smith, Timothy L.</au><au>Pillsbury, Harold C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide synthase is an active enzyme in the spiral ganglion cells of the rat cochlea</atitle><jtitle>Hearing research</jtitle><addtitle>Hear Res</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>79</volume><issue>1</issue><spage>39</spage><epage>47</epage><pages>39-47</pages><issn>0378-5955</issn><eissn>1878-5891</eissn><coden>HERED3</coden><abstract>Nitric oxide (NO) mediates the effects of the excitatory amino acids in the central nervous system. Excitatory amino acids, in particular
l-glutamate, are thought to be the neurotransmitter(s) present at the cochlear hair cell-afferent nerve synapse. To our knowledge, no studies to date have documented the presence of NO in the cochlea nor attempted to elucidate the role of NO in hearing. Rat cochlea frozen sections were examined for the presence of nitric oxide synthase (NOS) by NADPH diaphorase histochemistry. Vibratome sections of rat cochlea were examined by immunocytochemistry with an antibody to citrulline, an indication of NOS activity. Spiral ganglion cells in the rat cochlea were positive by NADPH diaphorase histochemistry and by anti-citrulline immunocytochemistry. These results indicate that NOS is present and that the enzyme actively produces nitric oxide in the spiral ganglion cells of the rat cochlea. Given our current understanding of neurotransmission in the cochlea, it is reasonable to postulate that the actions of NO in cochlear neuronal tissue are similar to the actions of NO in the CNS and that NO acts as a neurotransmitter/neuromodulator in the cochlea. In addition, because NO has been implicated as a mediator of excitotoxicity in the CNS, NO may play a role in neurotoxicity in the cochlea.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7528738</pmid><doi>10.1016/0378-5955(94)90125-2</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Hearing research, 1994-09, Vol.79 (1), p.39-47 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Oxidoreductases - metabolism Animals Antibodies, Monoclonal - immunology Biological and medical sciences Citrulline Citrulline - analysis Citrulline - immunology Cochlea - enzymology Cochlea - innervation Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation Frozen Sections Fundamental and applied biological sciences. Psychology Hair Cells, Auditory - cytology Hair Cells, Auditory - physiology Immunohistochemistry In Vitro Techniques Male NADPH Dehydrogenase - chemistry Neuronal NADPH diaphorase Neurotoxicity Neurotransmission Nitric Oxide - biosynthesis Nitric Oxide Synthase Prosencephalon - cytology Prosencephalon - enzymology Rats Rats, Sprague-Dawley Spiral Ganglion - cytology Spiral Ganglion - enzymology Spiral ganglion cells Synaptic Transmission - physiology Vertebrates: nervous system and sense organs |
| title | Nitric oxide synthase is an active enzyme in the spiral ganglion cells of the rat cochlea |
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