Protein phosphorylation in the blood-brain barrier. Possible presence of marcks in brain microvessels
The protein phosphorylation in rat brain microvessels has been examined; the major phosphorylated proteins correspond to a doublet of molecular weight 134–141 kDa, and four proteins of approx. 25, 55, 80 and 200 kDa. TPA (12- O-tetradecanoylphorbol-13-acetate) enhanced, in a few minutes, the phospho...
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| Veröffentlicht in: | Neurochemistry international 1996, Vol.28 (1), p.59-65 |
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| creator | Catalán, R.Edgardo Martínez, Ana M. Aragonés, M.Dolores Hernandez, Félix |
| description | The protein phosphorylation in rat brain microvessels has been examined; the major phosphorylated proteins correspond to a doublet of molecular weight 134–141 kDa, and four proteins of approx. 25, 55, 80 and 200 kDa. TPA (12-
O-tetradecanoylphorbol-13-acetate) enhanced, in a few minutes, the phosphorylation of three major protein substrates with apparent molecular weights of 17.5, 44.5 and 80 kDa. These effects are inhibited by staurosporine. The 80 kDa protein resulted to be myristoylated alanine-rich C kinase substrate (MARCKS). This work demonstrates that protein kinase C plays an important role in protein phosphorylation in blood-brain barrier (BBB). |
| doi_str_mv | 10.1016/0197-0186(95)00060-L |
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O-tetradecanoylphorbol-13-acetate) enhanced, in a few minutes, the phosphorylation of three major protein substrates with apparent molecular weights of 17.5, 44.5 and 80 kDa. These effects are inhibited by staurosporine. The 80 kDa protein resulted to be myristoylated alanine-rich C kinase substrate (MARCKS). This work demonstrates that protein kinase C plays an important role in protein phosphorylation in blood-brain barrier (BBB).</description><identifier>ISSN: 0197-0186</identifier><identifier>EISSN: 1872-9754</identifier><identifier>DOI: 10.1016/0197-0186(95)00060-L</identifier><identifier>PMID: 8746765</identifier><identifier>CODEN: NEUIDS</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Alkaloids - pharmacology ; Animals ; Autoradiography ; Biological and medical sciences ; Blood-Brain Barrier - drug effects ; Blood-Brain Barrier - physiology ; Brain - blood supply ; Capillaries - drug effects ; Capillaries - metabolism ; Capillaries - physiology ; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges ; Cerebrovascular Circulation - physiology ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Intracellular Signaling Peptides and Proteins ; Male ; Membrane Proteins ; Molecular Weight ; Myristoylated Alanine-Rich C Kinase Substrate ; Nerve Tissue Proteins - metabolism ; Phosphoproteins - metabolism ; Phosphorus Radioisotopes ; Phosphorylation ; Protein Kinase C - antagonists & inhibitors ; Protein Kinase C - metabolism ; Proteins - metabolism ; Proteins - physiology ; Rats ; Rats, Wistar ; Staurosporine ; Tetradecanoylphorbol Acetate - pharmacology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neurochemistry international, 1996, Vol.28 (1), p.59-65</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-6d0739a965d60712f7fcd127e255e47a5d04760b5f703737914aa6995ef57c3a3</citedby><cites>FETCH-LOGICAL-c417t-6d0739a965d60712f7fcd127e255e47a5d04760b5f703737914aa6995ef57c3a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0197-0186(95)00060-L$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2961742$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8746765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Catalán, R.Edgardo</creatorcontrib><creatorcontrib>Martínez, Ana M.</creatorcontrib><creatorcontrib>Aragonés, M.Dolores</creatorcontrib><creatorcontrib>Hernandez, Félix</creatorcontrib><title>Protein phosphorylation in the blood-brain barrier. Possible presence of marcks in brain microvessels</title><title>Neurochemistry international</title><addtitle>Neurochem Int</addtitle><description>The protein phosphorylation in rat brain microvessels has been examined; the major phosphorylated proteins correspond to a doublet of molecular weight 134–141 kDa, and four proteins of approx. 25, 55, 80 and 200 kDa. TPA (12-
O-tetradecanoylphorbol-13-acetate) enhanced, in a few minutes, the phosphorylation of three major protein substrates with apparent molecular weights of 17.5, 44.5 and 80 kDa. These effects are inhibited by staurosporine. The 80 kDa protein resulted to be myristoylated alanine-rich C kinase substrate (MARCKS). This work demonstrates that protein kinase C plays an important role in protein phosphorylation in blood-brain barrier (BBB).</description><subject>Alkaloids - pharmacology</subject><subject>Animals</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Blood-Brain Barrier - drug effects</subject><subject>Blood-Brain Barrier - physiology</subject><subject>Brain - blood supply</subject><subject>Capillaries - drug effects</subject><subject>Capillaries - metabolism</subject><subject>Capillaries - physiology</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Cerebrovascular Circulation - physiology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Male</subject><subject>Membrane Proteins</subject><subject>Molecular Weight</subject><subject>Myristoylated Alanine-Rich C Kinase Substrate</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorus Radioisotopes</subject><subject>Phosphorylation</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Protein Kinase C - metabolism</subject><subject>Proteins - metabolism</subject><subject>Proteins - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Staurosporine</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0197-0186</issn><issn>1872-9754</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A4UeRPRQTdoms7kIIn7Bgh70HNJ0itFus2a6gv_e1F326CGEZJ53MnkYOxb8UnChrrjQkHMxVedaXnDOFc9nW2wiplDkGmS1zSYbZI_tE30kCDSXu2x3CpUCJScMX2IY0PfZ4j1QWvGns4MPfZauhnfM6i6EJq-jTefaxugxXmYvgcjXHWaLiIS9wyy02dxG90ljbkXPvYvhG4mwo0O209qO8Gi9H7C3-7vX28d89vzwdHszy10lYMhVw6HUVivZKA6iaKF1jSgACymxAisbXoHitWyBl1CCFpW1SmuJrQRX2vKAna36LmL4WiINZu7JYdfZHsOSjABeFCUUCaxWYJqRKGJrFtGnH_wYwc1o14zqzKjOaGn-7JpZip2s-y_rOTab0Fpnqp-u65ac7dpoe-dpgxVaCajG169XWFKD30mpIedHj42P6AbTBP__HL9-spZV</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Catalán, R.Edgardo</creator><creator>Martínez, Ana M.</creator><creator>Aragonés, M.Dolores</creator><creator>Hernandez, Félix</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>1996</creationdate><title>Protein phosphorylation in the blood-brain barrier. Possible presence of marcks in brain microvessels</title><author>Catalán, R.Edgardo ; Martínez, Ana M. ; Aragonés, M.Dolores ; Hernandez, Félix</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-6d0739a965d60712f7fcd127e255e47a5d04760b5f703737914aa6995ef57c3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Alkaloids - pharmacology</topic><topic>Animals</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Blood-Brain Barrier - drug effects</topic><topic>Blood-Brain Barrier - physiology</topic><topic>Brain - blood supply</topic><topic>Capillaries - drug effects</topic><topic>Capillaries - metabolism</topic><topic>Capillaries - physiology</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Cerebrovascular Circulation - physiology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>Male</topic><topic>Membrane Proteins</topic><topic>Molecular Weight</topic><topic>Myristoylated Alanine-Rich C Kinase Substrate</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorus Radioisotopes</topic><topic>Phosphorylation</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Protein Kinase C - metabolism</topic><topic>Proteins - metabolism</topic><topic>Proteins - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Staurosporine</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Catalán, R.Edgardo</creatorcontrib><creatorcontrib>Martínez, Ana M.</creatorcontrib><creatorcontrib>Aragonés, M.Dolores</creatorcontrib><creatorcontrib>Hernandez, Félix</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neurochemistry international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Catalán, R.Edgardo</au><au>Martínez, Ana M.</au><au>Aragonés, M.Dolores</au><au>Hernandez, Félix</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein phosphorylation in the blood-brain barrier. Possible presence of marcks in brain microvessels</atitle><jtitle>Neurochemistry international</jtitle><addtitle>Neurochem Int</addtitle><date>1996</date><risdate>1996</risdate><volume>28</volume><issue>1</issue><spage>59</spage><epage>65</epage><pages>59-65</pages><issn>0197-0186</issn><eissn>1872-9754</eissn><coden>NEUIDS</coden><abstract>The protein phosphorylation in rat brain microvessels has been examined; the major phosphorylated proteins correspond to a doublet of molecular weight 134–141 kDa, and four proteins of approx. 25, 55, 80 and 200 kDa. TPA (12-
O-tetradecanoylphorbol-13-acetate) enhanced, in a few minutes, the phosphorylation of three major protein substrates with apparent molecular weights of 17.5, 44.5 and 80 kDa. These effects are inhibited by staurosporine. The 80 kDa protein resulted to be myristoylated alanine-rich C kinase substrate (MARCKS). This work demonstrates that protein kinase C plays an important role in protein phosphorylation in blood-brain barrier (BBB).</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8746765</pmid><doi>10.1016/0197-0186(95)00060-L</doi><tpages>7</tpages></addata></record> |
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| ispartof | Neurochemistry international, 1996, Vol.28 (1), p.59-65 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkaloids - pharmacology Animals Autoradiography Biological and medical sciences Blood-Brain Barrier - drug effects Blood-Brain Barrier - physiology Brain - blood supply Capillaries - drug effects Capillaries - metabolism Capillaries - physiology Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Cerebrovascular Circulation - physiology Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology In Vitro Techniques Intracellular Signaling Peptides and Proteins Male Membrane Proteins Molecular Weight Myristoylated Alanine-Rich C Kinase Substrate Nerve Tissue Proteins - metabolism Phosphoproteins - metabolism Phosphorus Radioisotopes Phosphorylation Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism Proteins - metabolism Proteins - physiology Rats Rats, Wistar Staurosporine Tetradecanoylphorbol Acetate - pharmacology Vertebrates: nervous system and sense organs |
| title | Protein phosphorylation in the blood-brain barrier. Possible presence of marcks in brain microvessels |
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