Intraspinal Transplantation of Autologous Neurogenically-Induced Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Paraplegic Dogs without Deep Pain Perception Secondary to Intervertebral Disk Disease
To investigate the effects of neurogenically-induced autologous bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in paraplegic dogs without deep pain perception (DPP) secondary to intervertebral disk disease (IVDD). Seven dogs which could not be improved neurologically with conventional treatm...
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| Veröffentlicht in: | Turkish neurosurgery 2015, Vol.25 (4), p.625-632 |
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| creator | Besalti, Omer Can, Pinar Akpinar, Eylul Aktas, Zeynep Elcin, Ayse Eser Elcin, Yasar Murat |
| description | To investigate the effects of neurogenically-induced autologous bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in paraplegic dogs without deep pain perception (DPP) secondary to intervertebral disk disease (IVDD).
Seven dogs which could not be improved neurologically with conventional treatment modalities were included in the study. All dogs were diagnosed by magnetic resonance imaging and surgically treated. Each dog received two times a suspension of autologous 5.0x106 NIBM-MSCs, which were positive to CNPase and MAP-2, as well as to GFAP and beta III tubulin into the spinal cord through the hemilaminectomy defect percutaneously, with a 21-day interval.
Two months after cell transplantation, there were no changes except for 1 gait score improvement for 1 of the cases. At the 4th month, gait score had improved 1 score in 5 cases, and one score progress was recorded in proprioception and nociception in 1 case. In eight months-followed up 4 cases were evaluated by the same parameter; gait score had improved in 3 cases, and propriception improved in 2 cases, and nociception improved in 3 cases.
Our findings suggest that utility of autologous NIBM-MSCs for cases with poor prognosis after IVDD can be a promising approach. |
| doi_str_mv | 10.5137/1019-5149.JTN.14502-15.2 |
| format | Article |
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Seven dogs which could not be improved neurologically with conventional treatment modalities were included in the study. All dogs were diagnosed by magnetic resonance imaging and surgically treated. Each dog received two times a suspension of autologous 5.0x106 NIBM-MSCs, which were positive to CNPase and MAP-2, as well as to GFAP and beta III tubulin into the spinal cord through the hemilaminectomy defect percutaneously, with a 21-day interval.
Two months after cell transplantation, there were no changes except for 1 gait score improvement for 1 of the cases. At the 4th month, gait score had improved 1 score in 5 cases, and one score progress was recorded in proprioception and nociception in 1 case. In eight months-followed up 4 cases were evaluated by the same parameter; gait score had improved in 3 cases, and propriception improved in 2 cases, and nociception improved in 3 cases.
Our findings suggest that utility of autologous NIBM-MSCs for cases with poor prognosis after IVDD can be a promising approach.</description><identifier>ISSN: 1019-5149</identifier><identifier>DOI: 10.5137/1019-5149.JTN.14502-15.2</identifier><identifier>PMID: 26242341</identifier><language>eng</language><publisher>Turkey</publisher><subject>2',3'-Cyclic-Nucleotide Phosphodiesterases - analysis ; 2',3'-Cyclic-Nucleotide Phosphodiesterases - metabolism ; Animals ; Bone Marrow Cells ; Dog Diseases - therapy ; Dogs ; Glial Fibrillary Acidic Protein - metabolism ; Intervertebral Disc Degeneration - complications ; Intervertebral Disc Degeneration - therapy ; Intervertebral Disc Degeneration - veterinary ; Intervertebral Disc Displacement - complications ; Intervertebral Disc Displacement - therapy ; Intervertebral Disc Displacement - veterinary ; Lameness, Animal - etiology ; Lameness, Animal - therapy ; Magnetic Resonance Imaging ; Mesenchymal Stem Cell Transplantation - methods ; Mitogen-Activated Protein Kinase 1 - metabolism ; Neural Stem Cells ; Neurologic Examination ; Pain Perception ; Paraplegia - etiology ; Paraplegia - therapy ; Paraplegia - veterinary ; Spinal Cord - surgery ; Transplantation, Autologous ; Treatment Outcome ; Tubulin - metabolism</subject><ispartof>Turkish neurosurgery, 2015, Vol.25 (4), p.625-632</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-52a62f24c91b4aafb20f4f7e730b34e5b0236d8f45069f24dd30bae1359ba05f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26242341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Besalti, Omer</creatorcontrib><creatorcontrib>Can, Pinar</creatorcontrib><creatorcontrib>Akpinar, Eylul</creatorcontrib><creatorcontrib>Aktas, Zeynep</creatorcontrib><creatorcontrib>Elcin, Ayse Eser</creatorcontrib><creatorcontrib>Elcin, Yasar Murat</creatorcontrib><title>Intraspinal Transplantation of Autologous Neurogenically-Induced Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Paraplegic Dogs without Deep Pain Perception Secondary to Intervertebral Disk Disease</title><title>Turkish neurosurgery</title><addtitle>Turk Neurosurg</addtitle><description>To investigate the effects of neurogenically-induced autologous bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in paraplegic dogs without deep pain perception (DPP) secondary to intervertebral disk disease (IVDD).
Seven dogs which could not be improved neurologically with conventional treatment modalities were included in the study. All dogs were diagnosed by magnetic resonance imaging and surgically treated. Each dog received two times a suspension of autologous 5.0x106 NIBM-MSCs, which were positive to CNPase and MAP-2, as well as to GFAP and beta III tubulin into the spinal cord through the hemilaminectomy defect percutaneously, with a 21-day interval.
Two months after cell transplantation, there were no changes except for 1 gait score improvement for 1 of the cases. At the 4th month, gait score had improved 1 score in 5 cases, and one score progress was recorded in proprioception and nociception in 1 case. In eight months-followed up 4 cases were evaluated by the same parameter; gait score had improved in 3 cases, and propriception improved in 2 cases, and nociception improved in 3 cases.
Our findings suggest that utility of autologous NIBM-MSCs for cases with poor prognosis after IVDD can be a promising approach.</description><subject>2',3'-Cyclic-Nucleotide Phosphodiesterases - analysis</subject><subject>2',3'-Cyclic-Nucleotide Phosphodiesterases - metabolism</subject><subject>Animals</subject><subject>Bone Marrow Cells</subject><subject>Dog Diseases - therapy</subject><subject>Dogs</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Intervertebral Disc Degeneration - complications</subject><subject>Intervertebral Disc Degeneration - therapy</subject><subject>Intervertebral Disc Degeneration - veterinary</subject><subject>Intervertebral Disc Displacement - complications</subject><subject>Intervertebral Disc Displacement - therapy</subject><subject>Intervertebral Disc Displacement - veterinary</subject><subject>Lameness, Animal - etiology</subject><subject>Lameness, Animal - therapy</subject><subject>Magnetic Resonance Imaging</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Neural Stem Cells</subject><subject>Neurologic Examination</subject><subject>Pain Perception</subject><subject>Paraplegia - etiology</subject><subject>Paraplegia - therapy</subject><subject>Paraplegia - veterinary</subject><subject>Spinal Cord - surgery</subject><subject>Transplantation, Autologous</subject><subject>Treatment Outcome</subject><subject>Tubulin - metabolism</subject><issn>1019-5149</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9Uctu2zAQ1KFFkqb5hYLHXuSSFCVZx9RuWgd5AXHOxIpa2WwpUiWpBP7Q_k-oJM2FC3BmdnZ3sowwuihZUX9jlDV5yUSzuNzeLJgoKc9ZueAfspN36Dj7FMJvSquKM3aUHfOKC14IdpL929joIYzagiFbDzaMBmyEqJ0lrifnU3TG7dwUyA1O3u3QagXGHPKN7SaFHfnuLJJr8N495Wv0-jH9XWNAq_aHITW9jziQFRoTiLYk7jHZIMQBbZwN7sDDaHCnFVm7XSBPOu7dFMkacUxgktyhVzi-DHSPytkO_IFER9Lk6B_RR2x98lnr8Gd-EAJ-zj72YAKevdXT7OHix3b1K7-6_blZnV_lShQs5iWHivdcqIa1AqBvOe1FX2Nd0LYQWLaUF1W37NNNqybxui4BgKwomxZo2Ren2dfXvqN3fycMUQ46qLQrWEwnk6ymnC5rwetEXb5SlXcheOzl6PWQVpGMyjlIOacl57RkClK-BClZKXmSfnlzmdoBu3fh_xSLZzhroY0</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Besalti, Omer</creator><creator>Can, Pinar</creator><creator>Akpinar, Eylul</creator><creator>Aktas, Zeynep</creator><creator>Elcin, Ayse Eser</creator><creator>Elcin, Yasar Murat</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2015</creationdate><title>Intraspinal Transplantation of Autologous Neurogenically-Induced Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Paraplegic Dogs without Deep Pain Perception Secondary to Intervertebral Disk Disease</title><author>Besalti, Omer ; Can, Pinar ; Akpinar, Eylul ; Aktas, Zeynep ; Elcin, Ayse Eser ; Elcin, Yasar Murat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-52a62f24c91b4aafb20f4f7e730b34e5b0236d8f45069f24dd30bae1359ba05f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>2',3'-Cyclic-Nucleotide Phosphodiesterases - analysis</topic><topic>2',3'-Cyclic-Nucleotide Phosphodiesterases - metabolism</topic><topic>Animals</topic><topic>Bone Marrow Cells</topic><topic>Dog Diseases - therapy</topic><topic>Dogs</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Intervertebral Disc Degeneration - complications</topic><topic>Intervertebral Disc Degeneration - therapy</topic><topic>Intervertebral Disc Degeneration - veterinary</topic><topic>Intervertebral Disc Displacement - complications</topic><topic>Intervertebral Disc Displacement - therapy</topic><topic>Intervertebral Disc Displacement - veterinary</topic><topic>Lameness, Animal - etiology</topic><topic>Lameness, Animal - therapy</topic><topic>Magnetic Resonance Imaging</topic><topic>Mesenchymal Stem Cell Transplantation - methods</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Neural Stem Cells</topic><topic>Neurologic Examination</topic><topic>Pain Perception</topic><topic>Paraplegia - etiology</topic><topic>Paraplegia - therapy</topic><topic>Paraplegia - veterinary</topic><topic>Spinal Cord - surgery</topic><topic>Transplantation, Autologous</topic><topic>Treatment Outcome</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Besalti, Omer</creatorcontrib><creatorcontrib>Can, Pinar</creatorcontrib><creatorcontrib>Akpinar, Eylul</creatorcontrib><creatorcontrib>Aktas, Zeynep</creatorcontrib><creatorcontrib>Elcin, Ayse Eser</creatorcontrib><creatorcontrib>Elcin, Yasar Murat</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Turkish neurosurgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Besalti, Omer</au><au>Can, Pinar</au><au>Akpinar, Eylul</au><au>Aktas, Zeynep</au><au>Elcin, Ayse Eser</au><au>Elcin, Yasar Murat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intraspinal Transplantation of Autologous Neurogenically-Induced Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Paraplegic Dogs without Deep Pain Perception Secondary to Intervertebral Disk Disease</atitle><jtitle>Turkish neurosurgery</jtitle><addtitle>Turk Neurosurg</addtitle><date>2015</date><risdate>2015</risdate><volume>25</volume><issue>4</issue><spage>625</spage><epage>632</epage><pages>625-632</pages><issn>1019-5149</issn><abstract>To investigate the effects of neurogenically-induced autologous bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in paraplegic dogs without deep pain perception (DPP) secondary to intervertebral disk disease (IVDD).
Seven dogs which could not be improved neurologically with conventional treatment modalities were included in the study. All dogs were diagnosed by magnetic resonance imaging and surgically treated. Each dog received two times a suspension of autologous 5.0x106 NIBM-MSCs, which were positive to CNPase and MAP-2, as well as to GFAP and beta III tubulin into the spinal cord through the hemilaminectomy defect percutaneously, with a 21-day interval.
Two months after cell transplantation, there were no changes except for 1 gait score improvement for 1 of the cases. At the 4th month, gait score had improved 1 score in 5 cases, and one score progress was recorded in proprioception and nociception in 1 case. In eight months-followed up 4 cases were evaluated by the same parameter; gait score had improved in 3 cases, and propriception improved in 2 cases, and nociception improved in 3 cases.
Our findings suggest that utility of autologous NIBM-MSCs for cases with poor prognosis after IVDD can be a promising approach.</abstract><cop>Turkey</cop><pmid>26242341</pmid><doi>10.5137/1019-5149.JTN.14502-15.2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 2',3'-Cyclic-Nucleotide Phosphodiesterases - analysis 2',3'-Cyclic-Nucleotide Phosphodiesterases - metabolism Animals Bone Marrow Cells Dog Diseases - therapy Dogs Glial Fibrillary Acidic Protein - metabolism Intervertebral Disc Degeneration - complications Intervertebral Disc Degeneration - therapy Intervertebral Disc Degeneration - veterinary Intervertebral Disc Displacement - complications Intervertebral Disc Displacement - therapy Intervertebral Disc Displacement - veterinary Lameness, Animal - etiology Lameness, Animal - therapy Magnetic Resonance Imaging Mesenchymal Stem Cell Transplantation - methods Mitogen-Activated Protein Kinase 1 - metabolism Neural Stem Cells Neurologic Examination Pain Perception Paraplegia - etiology Paraplegia - therapy Paraplegia - veterinary Spinal Cord - surgery Transplantation, Autologous Treatment Outcome Tubulin - metabolism |
| title | Intraspinal Transplantation of Autologous Neurogenically-Induced Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Paraplegic Dogs without Deep Pain Perception Secondary to Intervertebral Disk Disease |
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