Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging

The simultaneous observation of multiple fluorescent proteins (FPs) by optical microscopy is revealing mechanisms by which proteins and organelles control a variety of cellular functions. Here we show the use of visible-light based two-photon excitation for simultaneously imaging multiple FPs. We de...

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Veröffentlicht in:	Journal of biomedical optics 2015-10, Vol.20 (10), p.101202-101202
Hauptverfasser:	Yamanaka, Masahito, Saito, Kenta, Smith, Nicholas I, Arai, Yoshiyuki, Uegaki, Kumiko, Yonemaru, Yasuo, Mochizuki, Kentaro, Kawata, Satoshi, Nagai, Takeharu, Fujita, Katsumasa
Format:	Artikel
Sprache:	eng
Schlagworte:	Equipment Design Equipment Failure Analysis HeLa Cells Humans Image Enhancement - instrumentation Luminescent Proteins - metabolism Microscopy, Fluorescence, Multiphoton - instrumentation Molecular Imaging - instrumentation Reproducibility of Results Sensitivity and Specificity
Online-Zugang:	Volltext
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