Anti-Apoptotic Effects of Dapsone After Spinal Cord Injury in Rats
Spinal cord injury (SCI) is a condition producing irreversible damage to the neurological function. Among the leading mechanisms associated to cell death after SCI, excitotoxicity, oxidative stress, inflammatory response and apoptosis are considered potential targets to prevent tissue damage. We rec...
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| Veröffentlicht in: | Neurochemical research 2015-06, Vol.40 (6), p.1243-1251 |
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| creator | Ríos, Camilo Orozco-Suarez, Sandra Salgado-Ceballos, Hermelinda Mendez-Armenta, Marisela Nava-Ruiz, Concepción Santander, Iván Barón-Flores, Veronica Caram-Salas, Nadia Diaz-Ruiz, Araceli |
| description | Spinal cord injury (SCI) is a condition producing irreversible damage to the neurological function. Among the leading mechanisms associated to cell death after SCI, excitotoxicity, oxidative stress, inflammatory response and apoptosis are considered potential targets to prevent tissue damage. We recently reported that dapsone an anti-inflammatory drug, decreases the activity of myeloperoxidase, lipid peroxidation, improve neurological function and increase the amount of spared tissue after SCI in rats. In this study, we characterized the anti-apoptotic effect of dapsone administered at 12.5 mg/kg/24 h dose, starting at 3 and 5 h after SCI. We monitored the activity of caspases-8, 9, and 3 and quantitated Annexin V and TUNEL positive cells in the core of the lesion. Results showed increased activities of caspase-8, 9 and 3 at 72 h by SCI to reach increments of 69, 143 and 293 %, respectively, as compared to sham group. Meanwhile, dapsone, administered at 3 and 5 after SCI, reduced caspase-8 activity by 36 and 44 % respectively, whereas the activity of caspase-9 was diminished by 37 %. Likewise, the activity of caspase-3 showed a decrease of 38 %. Finally, both Annexin V and TUNEL-positive cells were significantly reduced by DDS as compared to untreated SCI animals. Results showed that dapsone exerted anti-apoptotic effect after SCI. |
| doi_str_mv | 10.1007/s11064-015-1588-z |
| format | Article |
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Among the leading mechanisms associated to cell death after SCI, excitotoxicity, oxidative stress, inflammatory response and apoptosis are considered potential targets to prevent tissue damage. We recently reported that dapsone an anti-inflammatory drug, decreases the activity of myeloperoxidase, lipid peroxidation, improve neurological function and increase the amount of spared tissue after SCI in rats. In this study, we characterized the anti-apoptotic effect of dapsone administered at 12.5 mg/kg/24 h dose, starting at 3 and 5 h after SCI. We monitored the activity of caspases-8, 9, and 3 and quantitated Annexin V and TUNEL positive cells in the core of the lesion. Results showed increased activities of caspase-8, 9 and 3 at 72 h by SCI to reach increments of 69, 143 and 293 %, respectively, as compared to sham group. Meanwhile, dapsone, administered at 3 and 5 after SCI, reduced caspase-8 activity by 36 and 44 % respectively, whereas the activity of caspase-9 was diminished by 37 %. Likewise, the activity of caspase-3 showed a decrease of 38 %. Finally, both Annexin V and TUNEL-positive cells were significantly reduced by DDS as compared to untreated SCI animals. Results showed that dapsone exerted anti-apoptotic effect after SCI.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-015-1588-z</identifier><identifier>PMID: 25931161</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Apoptosis - drug effects ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Caspases - metabolism ; Cell Biology ; Dapsone - pharmacology ; Dapsone - therapeutic use ; Female ; Folic Acid Antagonists - pharmacology ; Folic Acid Antagonists - therapeutic use ; In Situ Nick-End Labeling ; Neurochemistry ; Neurology ; Neurosciences ; Original Paper ; Oxidative Stress - drug effects ; Rats ; Rats, Wistar ; Recovery of Function - drug effects ; Spinal Cord Injuries - drug therapy ; Spinal Cord Injuries - enzymology ; Spinal Cord Injuries - pathology</subject><ispartof>Neurochemical research, 2015-06, Vol.40 (6), p.1243-1251</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-236d8c7f4449362ac9dd65dbbb694bb3b08560a170baf722ff0109d08e085e033</citedby><cites>FETCH-LOGICAL-c475t-236d8c7f4449362ac9dd65dbbb694bb3b08560a170baf722ff0109d08e085e033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11064-015-1588-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11064-015-1588-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25931161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ríos, Camilo</creatorcontrib><creatorcontrib>Orozco-Suarez, Sandra</creatorcontrib><creatorcontrib>Salgado-Ceballos, Hermelinda</creatorcontrib><creatorcontrib>Mendez-Armenta, Marisela</creatorcontrib><creatorcontrib>Nava-Ruiz, Concepción</creatorcontrib><creatorcontrib>Santander, Iván</creatorcontrib><creatorcontrib>Barón-Flores, Veronica</creatorcontrib><creatorcontrib>Caram-Salas, Nadia</creatorcontrib><creatorcontrib>Diaz-Ruiz, Araceli</creatorcontrib><title>Anti-Apoptotic Effects of Dapsone After Spinal Cord Injury in Rats</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><addtitle>Neurochem Res</addtitle><description>Spinal cord injury (SCI) is a condition producing irreversible damage to the neurological function. Among the leading mechanisms associated to cell death after SCI, excitotoxicity, oxidative stress, inflammatory response and apoptosis are considered potential targets to prevent tissue damage. We recently reported that dapsone an anti-inflammatory drug, decreases the activity of myeloperoxidase, lipid peroxidation, improve neurological function and increase the amount of spared tissue after SCI in rats. In this study, we characterized the anti-apoptotic effect of dapsone administered at 12.5 mg/kg/24 h dose, starting at 3 and 5 h after SCI. We monitored the activity of caspases-8, 9, and 3 and quantitated Annexin V and TUNEL positive cells in the core of the lesion. Results showed increased activities of caspase-8, 9 and 3 at 72 h by SCI to reach increments of 69, 143 and 293 %, respectively, as compared to sham group. Meanwhile, dapsone, administered at 3 and 5 after SCI, reduced caspase-8 activity by 36 and 44 % respectively, whereas the activity of caspase-9 was diminished by 37 %. Likewise, the activity of caspase-3 showed a decrease of 38 %. Finally, both Annexin V and TUNEL-positive cells were significantly reduced by DDS as compared to untreated SCI animals. Results showed that dapsone exerted anti-apoptotic effect after SCI.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Caspases - metabolism</subject><subject>Cell Biology</subject><subject>Dapsone - pharmacology</subject><subject>Dapsone - therapeutic use</subject><subject>Female</subject><subject>Folic Acid Antagonists - pharmacology</subject><subject>Folic Acid Antagonists - therapeutic use</subject><subject>In Situ Nick-End Labeling</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Original Paper</subject><subject>Oxidative Stress - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recovery of Function - drug effects</subject><subject>Spinal Cord Injuries - drug therapy</subject><subject>Spinal Cord Injuries - enzymology</subject><subject>Spinal Cord Injuries - 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drug effects</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Caspases - metabolism</topic><topic>Cell Biology</topic><topic>Dapsone - pharmacology</topic><topic>Dapsone - therapeutic use</topic><topic>Female</topic><topic>Folic Acid Antagonists - pharmacology</topic><topic>Folic Acid Antagonists - therapeutic use</topic><topic>In Situ Nick-End Labeling</topic><topic>Neurochemistry</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Original Paper</topic><topic>Oxidative Stress - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recovery of Function - drug effects</topic><topic>Spinal Cord Injuries - drug therapy</topic><topic>Spinal Cord Injuries - enzymology</topic><topic>Spinal Cord Injuries - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ríos, Camilo</creatorcontrib><creatorcontrib>Orozco-Suarez, Sandra</creatorcontrib><creatorcontrib>Salgado-Ceballos, Hermelinda</creatorcontrib><creatorcontrib>Mendez-Armenta, Marisela</creatorcontrib><creatorcontrib>Nava-Ruiz, Concepción</creatorcontrib><creatorcontrib>Santander, Iván</creatorcontrib><creatorcontrib>Barón-Flores, Veronica</creatorcontrib><creatorcontrib>Caram-Salas, Nadia</creatorcontrib><creatorcontrib>Diaz-Ruiz, Araceli</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ríos, Camilo</au><au>Orozco-Suarez, Sandra</au><au>Salgado-Ceballos, Hermelinda</au><au>Mendez-Armenta, Marisela</au><au>Nava-Ruiz, Concepción</au><au>Santander, Iván</au><au>Barón-Flores, Veronica</au><au>Caram-Salas, Nadia</au><au>Diaz-Ruiz, Araceli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-Apoptotic Effects of Dapsone After Spinal Cord Injury in Rats</atitle><jtitle>Neurochemical research</jtitle><stitle>Neurochem Res</stitle><addtitle>Neurochem Res</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>40</volume><issue>6</issue><spage>1243</spage><epage>1251</epage><pages>1243-1251</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><abstract>Spinal cord injury (SCI) is a condition producing irreversible damage to the neurological function. Among the leading mechanisms associated to cell death after SCI, excitotoxicity, oxidative stress, inflammatory response and apoptosis are considered potential targets to prevent tissue damage. We recently reported that dapsone an anti-inflammatory drug, decreases the activity of myeloperoxidase, lipid peroxidation, improve neurological function and increase the amount of spared tissue after SCI in rats. In this study, we characterized the anti-apoptotic effect of dapsone administered at 12.5 mg/kg/24 h dose, starting at 3 and 5 h after SCI. We monitored the activity of caspases-8, 9, and 3 and quantitated Annexin V and TUNEL positive cells in the core of the lesion. Results showed increased activities of caspase-8, 9 and 3 at 72 h by SCI to reach increments of 69, 143 and 293 %, respectively, as compared to sham group. Meanwhile, dapsone, administered at 3 and 5 after SCI, reduced caspase-8 activity by 36 and 44 % respectively, whereas the activity of caspase-9 was diminished by 37 %. Likewise, the activity of caspase-3 showed a decrease of 38 %. Finally, both Annexin V and TUNEL-positive cells were significantly reduced by DDS as compared to untreated SCI animals. Results showed that dapsone exerted anti-apoptotic effect after SCI.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25931161</pmid><doi>10.1007/s11064-015-1588-z</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Apoptosis - drug effects Biochemistry Biomedical and Life Sciences Biomedicine Caspases - metabolism Cell Biology Dapsone - pharmacology Dapsone - therapeutic use Female Folic Acid Antagonists - pharmacology Folic Acid Antagonists - therapeutic use In Situ Nick-End Labeling Neurochemistry Neurology Neurosciences Original Paper Oxidative Stress - drug effects Rats Rats, Wistar Recovery of Function - drug effects Spinal Cord Injuries - drug therapy Spinal Cord Injuries - enzymology Spinal Cord Injuries - pathology |
| title | Anti-Apoptotic Effects of Dapsone After Spinal Cord Injury in Rats |
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