Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway. The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared...
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| Veröffentlicht in: | Nature biotechnology 2015-05, Vol.33 (5), p.543-548 |
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| creator | Chu, Van Trung Weber, Timm Wefers, Benedikt Wurst, Wolfgang Sander, Sandrine Rajewsky, Klaus Kühn, Ralf |
| description | The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway.
The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared with the higher efficiency of the nonhomologous end-joining (NHEJ) pathway. To enhance HDR, enabling the insertion of precise genetic modifications, we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV by gene silencing, the ligase IV inhibitor SCR7 or the coexpression of adenovirus 4 E1B55K and E4orf6 proteins in a 'traffic light' and other reporter systems. Suppression of KU70 and DNA ligase IV promotes the efficiency of HDR 4–5-fold. When co-expressed with the Cas9 system, E1B55K and E4orf6 improved the efficiency of HDR up to eightfold and essentially abolished NHEJ activity in both human and mouse cell lines. Our findings provide useful tools to improve the frequency of precise gene modifications in mammalian cells. |
| doi_str_mv | 10.1038/nbt.3198 |
| format | Article |
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The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared with the higher efficiency of the nonhomologous end-joining (NHEJ) pathway. To enhance HDR, enabling the insertion of precise genetic modifications, we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV by gene silencing, the ligase IV inhibitor SCR7 or the coexpression of adenovirus 4 E1B55K and E4orf6 proteins in a 'traffic light' and other reporter systems. Suppression of KU70 and DNA ligase IV promotes the efficiency of HDR 4–5-fold. When co-expressed with the Cas9 system, E1B55K and E4orf6 improved the efficiency of HDR up to eightfold and essentially abolished NHEJ activity in both human and mouse cell lines. Our findings provide useful tools to improve the frequency of precise gene modifications in mammalian cells.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt.3198</identifier><identifier>PMID: 25803306</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/1647/1511 ; 631/1647/1513/1967/3196 ; 631/337/1427/2122 ; Adenoviridae - genetics ; Adenovirus ; Adenovirus E4 Proteins - biosynthesis ; Adenovirus E4 Proteins - genetics ; Agriculture ; Animals ; Bioinformatics ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Cell Line ; CRISPR-Cas Systems - genetics ; Deoxyribonucleic acid ; DNA ; DNA Breaks, Double-Stranded ; DNA End-Joining Repair - genetics ; DNA Ligase ATP ; DNA Ligases - genetics ; DNA repair ; Gene Expression Regulation ; Genetic engineering ; Genetic Engineering - methods ; Genetic research ; Genome, Human ; Homologous Recombination - genetics ; Humans ; Innovations ; letter ; Life Sciences ; Mammals ; Mice ; Viral Proteins - biosynthesis ; Viral Proteins - genetics</subject><ispartof>Nature biotechnology, 2015-05, Vol.33 (5), p.543-548</ispartof><rights>Springer Nature America, Inc. 2015</rights><rights>COPYRIGHT 2015 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group May 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c616t-e02c0cd2c1fb67a5b1cba7dd018e0b435912abd634ee8a52b9b7034d4276647d3</citedby><cites>FETCH-LOGICAL-c616t-e02c0cd2c1fb67a5b1cba7dd018e0b435912abd634ee8a52b9b7034d4276647d3</cites><orcidid>0000-0002-2492-9389 ; 0000000224929389</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt.3198$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt.3198$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25803306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chu, Van Trung</creatorcontrib><creatorcontrib>Weber, Timm</creatorcontrib><creatorcontrib>Wefers, Benedikt</creatorcontrib><creatorcontrib>Wurst, Wolfgang</creatorcontrib><creatorcontrib>Sander, Sandrine</creatorcontrib><creatorcontrib>Rajewsky, Klaus</creatorcontrib><creatorcontrib>Kühn, Ralf</creatorcontrib><title>Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway.
The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared with the higher efficiency of the nonhomologous end-joining (NHEJ) pathway. To enhance HDR, enabling the insertion of precise genetic modifications, we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV by gene silencing, the ligase IV inhibitor SCR7 or the coexpression of adenovirus 4 E1B55K and E4orf6 proteins in a 'traffic light' and other reporter systems. Suppression of KU70 and DNA ligase IV promotes the efficiency of HDR 4–5-fold. When co-expressed with the Cas9 system, E1B55K and E4orf6 improved the efficiency of HDR up to eightfold and essentially abolished NHEJ activity in both human and mouse cell lines. Our findings provide useful tools to improve the frequency of precise gene modifications in mammalian cells.</description><subject>631/1647/1511</subject><subject>631/1647/1513/1967/3196</subject><subject>631/337/1427/2122</subject><subject>Adenoviridae - genetics</subject><subject>Adenovirus</subject><subject>Adenovirus E4 Proteins - biosynthesis</subject><subject>Adenovirus E4 Proteins - genetics</subject><subject>Agriculture</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Breaks, Double-Stranded</subject><subject>DNA End-Joining Repair - genetics</subject><subject>DNA Ligase ATP</subject><subject>DNA Ligases - genetics</subject><subject>DNA repair</subject><subject>Gene Expression Regulation</subject><subject>Genetic engineering</subject><subject>Genetic Engineering - 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Academic</collection><jtitle>Nature biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chu, Van Trung</au><au>Weber, Timm</au><au>Wefers, Benedikt</au><au>Wurst, Wolfgang</au><au>Sander, Sandrine</au><au>Rajewsky, Klaus</au><au>Kühn, Ralf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>33</volume><issue>5</issue><spage>543</spage><epage>548</epage><pages>543-548</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><abstract>The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway.
The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared with the higher efficiency of the nonhomologous end-joining (NHEJ) pathway. To enhance HDR, enabling the insertion of precise genetic modifications, we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV by gene silencing, the ligase IV inhibitor SCR7 or the coexpression of adenovirus 4 E1B55K and E4orf6 proteins in a 'traffic light' and other reporter systems. Suppression of KU70 and DNA ligase IV promotes the efficiency of HDR 4–5-fold. When co-expressed with the Cas9 system, E1B55K and E4orf6 improved the efficiency of HDR up to eightfold and essentially abolished NHEJ activity in both human and mouse cell lines. Our findings provide useful tools to improve the frequency of precise gene modifications in mammalian cells.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>25803306</pmid><doi>10.1038/nbt.3198</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-2492-9389</orcidid><orcidid>https://orcid.org/0000000224929389</orcidid></addata></record> |
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| subjects | 631/1647/1511 631/1647/1513/1967/3196 631/337/1427/2122 Adenoviridae - genetics Adenovirus Adenovirus E4 Proteins - biosynthesis Adenovirus E4 Proteins - genetics Agriculture Animals Bioinformatics Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cell Line CRISPR-Cas Systems - genetics Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded DNA End-Joining Repair - genetics DNA Ligase ATP DNA Ligases - genetics DNA repair Gene Expression Regulation Genetic engineering Genetic Engineering - methods Genetic research Genome, Human Homologous Recombination - genetics Humans Innovations letter Life Sciences Mammals Mice Viral Proteins - biosynthesis Viral Proteins - genetics |
| title | Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells |
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