Xenopus laevis Vitellogenin Is a Zinc Protein
Vitellogenin induced by estrogen administration has been purified from the serum of Xenopus laevis. Six days after hormone injection, serum was collected and treated with 35% sat. (NH 4) 2SO 4 to remove globulins. A single vitellogenin containing fraction was isolated by chromatography on a Mono-Q c...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-05, Vol.200 (3), p.1407-1413 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Montorzi, M. Falchuk, K.H. Vallee, B.L. |
| description | Vitellogenin induced by estrogen administration has been purified from the serum of
Xenopus laevis. Six days after hormone injection, serum was collected and treated with 35% sat. (NH
4)
2SO
4 to remove globulins. A single vitellogenin containing fraction was isolated by chromatography on a Mono-Q column. The protein was identified on the basis of its amino acid composition. N-terminal sequence analysis and SDS-PAGE demonstrated the presence of two forms of vitellogenin (A and B). Consistent with the conclusions drawn from the teratology which chelating agents induce in frog embryos (1), metal analysis shows that vitellogenin is a zinc protein with 1 g atom zinc/220 kDa monomer but containing no other IIB and transition metals. |
| doi_str_mv | 10.1006/bbrc.1994.1607 |
| format | Article |
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Xenopus laevis. Six days after hormone injection, serum was collected and treated with 35% sat. (NH
4)
2SO
4 to remove globulins. A single vitellogenin containing fraction was isolated by chromatography on a Mono-Q column. The protein was identified on the basis of its amino acid composition. N-terminal sequence analysis and SDS-PAGE demonstrated the presence of two forms of vitellogenin (A and B). Consistent with the conclusions drawn from the teratology which chelating agents induce in frog embryos (1), metal analysis shows that vitellogenin is a zinc protein with 1 g atom zinc/220 kDa monomer but containing no other IIB and transition metals.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.1607</identifier><identifier>PMID: 8185593</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Metalloproteins ; Metalloproteins - chemistry ; Molecular Sequence Data ; Other metalloproteins ; Proteins ; Vitellogenins - chemistry ; Xenopus laevis</subject><ispartof>Biochemical and biophysical research communications, 1994-05, Vol.200 (3), p.1407-1413</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-2c1de4fbd28e2b1f8320e1ebea1eafe911737b99f2db9372c3dda7d980d710843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1994.1607$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4139647$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8185593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Montorzi, M.</creatorcontrib><creatorcontrib>Falchuk, K.H.</creatorcontrib><creatorcontrib>Vallee, B.L.</creatorcontrib><title>Xenopus laevis Vitellogenin Is a Zinc Protein</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Vitellogenin induced by estrogen administration has been purified from the serum of
Xenopus laevis. Six days after hormone injection, serum was collected and treated with 35% sat. (NH
4)
2SO
4 to remove globulins. A single vitellogenin containing fraction was isolated by chromatography on a Mono-Q column. The protein was identified on the basis of its amino acid composition. N-terminal sequence analysis and SDS-PAGE demonstrated the presence of two forms of vitellogenin (A and B). Consistent with the conclusions drawn from the teratology which chelating agents induce in frog embryos (1), metal analysis shows that vitellogenin is a zinc protein with 1 g atom zinc/220 kDa monomer but containing no other IIB and transition metals.</description><subject>Amino Acid Sequence</subject><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metalloproteins</subject><subject>Metalloproteins - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Other metalloproteins</subject><subject>Proteins</subject><subject>Vitellogenins - chemistry</subject><subject>Xenopus laevis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1Lw0AQhhdRaq1evQk5iLfUnWyazR5F_CgU9KBSvCz7MZGVdFN3k4L_3oSG3jzN4X3mZeYh5BLoHCgtbrUOZg5C5HMoKD8iU6CCphnQ_JhMaU-kmYD1KTmL8ZtSgLwQEzIpoVwsBJuSdI2-2XYxqRXuXEw-XIt13Xyhdz5ZxkQln86b5DU0LTp_Tk4qVUe8GOeMvD8-vN0_p6uXp-X93So1TIg2zQxYzCttsxIzDVXJMoqAGhWgqlAAcMa1EFVmtWA8M8xaxa0oqeVAy5zNyM2-dxuanw5jKzcumv4w5bHpogTef8L5ogfne9CEJsaAldwGt1HhVwKVgx85-JGDHzn46ReuxuZOb9Ae8FFIn1-PuYpG1VVQ3rh4wHJgosiHmnKPYW9h5zDIaBx6g9YFNK20jfvvgj9ToYAo</recordid><startdate>19940516</startdate><enddate>19940516</enddate><creator>Montorzi, M.</creator><creator>Falchuk, K.H.</creator><creator>Vallee, B.L.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>19940516</creationdate><title>Xenopus laevis Vitellogenin Is a Zinc Protein</title><author>Montorzi, M. ; Falchuk, K.H. ; Vallee, B.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-2c1de4fbd28e2b1f8320e1ebea1eafe911737b99f2db9372c3dda7d980d710843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metalloproteins</topic><topic>Metalloproteins - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Other metalloproteins</topic><topic>Proteins</topic><topic>Vitellogenins - chemistry</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Montorzi, M.</creatorcontrib><creatorcontrib>Falchuk, K.H.</creatorcontrib><creatorcontrib>Vallee, B.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Montorzi, M.</au><au>Falchuk, K.H.</au><au>Vallee, B.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Xenopus laevis Vitellogenin Is a Zinc Protein</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-05-16</date><risdate>1994</risdate><volume>200</volume><issue>3</issue><spage>1407</spage><epage>1413</epage><pages>1407-1413</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Vitellogenin induced by estrogen administration has been purified from the serum of
Xenopus laevis. Six days after hormone injection, serum was collected and treated with 35% sat. (NH
4)
2SO
4 to remove globulins. A single vitellogenin containing fraction was isolated by chromatography on a Mono-Q column. The protein was identified on the basis of its amino acid composition. N-terminal sequence analysis and SDS-PAGE demonstrated the presence of two forms of vitellogenin (A and B). Consistent with the conclusions drawn from the teratology which chelating agents induce in frog embryos (1), metal analysis shows that vitellogenin is a zinc protein with 1 g atom zinc/220 kDa monomer but containing no other IIB and transition metals.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8185593</pmid><doi>10.1006/bbrc.1994.1607</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1994-05, Vol.200 (3), p.1407-1413 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17011775 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Freshwater Fundamental and applied biological sciences. Psychology Metalloproteins Metalloproteins - chemistry Molecular Sequence Data Other metalloproteins Proteins Vitellogenins - chemistry Xenopus laevis |
| title | Xenopus laevis Vitellogenin Is a Zinc Protein |
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