Temporal focusing-based multiphoton excitation microscopy via digital micromirror device
This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the mi...
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| Veröffentlicht in: | Optics letters 2014-06, Vol.39 (11), p.3134-3137 |
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| container_issue | 11 |
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| container_title | Optics letters |
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| creator | Yih, Jenq-Nan Hu, Yvonne Yuling Sie, Yong Da Cheng, Li-Chung Lien, Chi-Hsiang Chen, Shean-Jen |
| description | This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a 600 lines/mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image-conjugate plane of the objective lens' focal plane, the MPE pattern can be projected on the focal plane precisely. |
| doi_str_mv | 10.1364/ol.39.003134 |
| format | Article |
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Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a 600 lines/mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. 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Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a 600 lines/mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image-conjugate plane of the objective lens' focal plane, the MPE pattern can be projected on the focal plane precisely.</abstract><cop>United States</cop><pmid>24875995</pmid><doi>10.1364/ol.39.003134</doi><tpages>4</tpages></addata></record> |
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| source | OSA Publishing; MEDLINE |
| subjects | Devices Diffraction gratings Digital Equipment Design Excitation Focal plane Gratings (spectra) Lenses Microscopes Microscopy, Fluorescence, Multiphoton - instrumentation Optical Devices Optical Phenomena Temporal logic |
| title | Temporal focusing-based multiphoton excitation microscopy via digital micromirror device |
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