Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters
In this work, a sensitive and selective ratiometric fluorescence sensing platform was built for the detection of tyrosinase (TYR) activity and dopamine (DA) using glutathione (GSH) protected gold nanoclusters (Au NCs) as probes. Upon excitation at 350 nm, Au NCs displayed an intense red emission, wh...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2015-05, Vol.87 (9), p.4897-4902 |
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| creator | Teng, Ye Jia, Xiaofang Li, Jing Wang, Erkang |
| description | In this work, a sensitive and selective ratiometric fluorescence sensing platform was built for the detection of tyrosinase (TYR) activity and dopamine (DA) using glutathione (GSH) protected gold nanoclusters (Au NCs) as probes. Upon excitation at 350 nm, Au NCs displayed an intense red emission, which could be effectively quenched by quinones. TYR, a typical polyphenol oxidase, can catalyze the oxidization of DA to o-quinone and therefore quenched the fluorescence of Au NCs. Moreover, the reaction of TYR and DA gave rise to an emission band at 400 nm, which increased in a TYR/DA-concentration-dependent manner. The ratiometric signal variations were utilized for facile, sensitive, and selective detection of TYR activity and DA. A linear range was obtained from 0.006–3.6 unit mL–1 of TYR activity, while the linear range for detection of DA was 1.0 nM to 1.0 mM. Additionally, it constructed a useful platform for TYR inhibitor screening in biomedical research. |
| doi_str_mv | 10.1021/acs.analchem.5b00468 |
| format | Article |
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Upon excitation at 350 nm, Au NCs displayed an intense red emission, which could be effectively quenched by quinones. TYR, a typical polyphenol oxidase, can catalyze the oxidization of DA to o-quinone and therefore quenched the fluorescence of Au NCs. Moreover, the reaction of TYR and DA gave rise to an emission band at 400 nm, which increased in a TYR/DA-concentration-dependent manner. The ratiometric signal variations were utilized for facile, sensitive, and selective detection of TYR activity and DA. A linear range was obtained from 0.006–3.6 unit mL–1 of TYR activity, while the linear range for detection of DA was 1.0 nM to 1.0 mM. Additionally, it constructed a useful platform for TYR inhibitor screening in biomedical research.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.5b00468</identifier><identifier>PMID: 25846058</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Biomedical research ; Dopamine ; Dopamine - analysis ; Emission ; Fluorescence ; Glutathione - chemistry ; Gold ; Gold - chemistry ; Inhibitors ; Metal Nanoparticles - chemistry ; Monophenol Monooxygenase - analysis ; Monophenol Monooxygenase - metabolism ; Nanostructure ; Platforms ; Quenching (cooling) ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet ; Tyrosinase</subject><ispartof>Analytical chemistry (Washington), 2015-05, Vol.87 (9), p.4897-4902</ispartof><rights>Copyright © American Chemical Society</rights><rights>Copyright American Chemical Society May 5, 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a475t-f5d8208e63ff58114bacb8b49fe9fce839463fd7f17e62eb1fb556e9c00085ae3</citedby><cites>FETCH-LOGICAL-a475t-f5d8208e63ff58114bacb8b49fe9fce839463fd7f17e62eb1fb556e9c00085ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.5b00468$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.5b00468$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25846058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teng, Ye</creatorcontrib><creatorcontrib>Jia, Xiaofang</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Wang, Erkang</creatorcontrib><title>Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>In this work, a sensitive and selective ratiometric fluorescence sensing platform was built for the detection of tyrosinase (TYR) activity and dopamine (DA) using glutathione (GSH) protected gold nanoclusters (Au NCs) as probes. Upon excitation at 350 nm, Au NCs displayed an intense red emission, which could be effectively quenched by quinones. TYR, a typical polyphenol oxidase, can catalyze the oxidization of DA to o-quinone and therefore quenched the fluorescence of Au NCs. Moreover, the reaction of TYR and DA gave rise to an emission band at 400 nm, which increased in a TYR/DA-concentration-dependent manner. The ratiometric signal variations were utilized for facile, sensitive, and selective detection of TYR activity and DA. A linear range was obtained from 0.006–3.6 unit mL–1 of TYR activity, while the linear range for detection of DA was 1.0 nM to 1.0 mM. Additionally, it constructed a useful platform for TYR inhibitor screening in biomedical research.</description><subject>Analytical chemistry</subject><subject>Biomedical research</subject><subject>Dopamine</subject><subject>Dopamine - analysis</subject><subject>Emission</subject><subject>Fluorescence</subject><subject>Glutathione - chemistry</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Inhibitors</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Monophenol Monooxygenase - analysis</subject><subject>Monophenol Monooxygenase - metabolism</subject><subject>Nanostructure</subject><subject>Platforms</subject><subject>Quenching (cooling)</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Tyrosinase</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq2qVVmg_wBVlnrhku1MEn_kiKBQJNRWaDlHjjOGoCRe7KTS_nu87NJKHNqTJc_zvtb4YewEYYmQ41dj49KMprcPNCxFA1BK_Y4tUOSQSa3z92wBAEWWK4ADdhjjIwAioPzIDnKhSwlCL9jTrZk6P9AUOssv-9kHipZGS_yCJrJpNnLv-GoTfOxGE4mfpcvf3bThZmz5hV-boRuJ36XpPV89dL43E2W_gt-mqeVXvm_5DzN6289xohCP2Qdn-kif9ucRu7v8tjr_nt38vLo-P7vJTKnElDnR6hw0ycI5oRHLxthGN2XlqHKWdFGVadQqh4pkTg26RghJlU1La2GoOGKnu9518E8zxakeurRa35uR_BxrVICgpET1f1RqQCXKYot-eYM--jkkDS8UVgVoVSSq3FE2fVsM5Op16AYTNjVCvbVXJ3v1q716by_FPu_L52ag9k_oVVcCYAds438f_lfnM2Wpqoc</recordid><startdate>20150505</startdate><enddate>20150505</enddate><creator>Teng, Ye</creator><creator>Jia, Xiaofang</creator><creator>Li, Jing</creator><creator>Wang, Erkang</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20150505</creationdate><title>Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters</title><author>Teng, Ye ; Jia, Xiaofang ; Li, Jing ; Wang, Erkang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a475t-f5d8208e63ff58114bacb8b49fe9fce839463fd7f17e62eb1fb556e9c00085ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analytical chemistry</topic><topic>Biomedical research</topic><topic>Dopamine</topic><topic>Dopamine - analysis</topic><topic>Emission</topic><topic>Fluorescence</topic><topic>Glutathione - chemistry</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Inhibitors</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Monophenol Monooxygenase - analysis</topic><topic>Monophenol Monooxygenase - metabolism</topic><topic>Nanostructure</topic><topic>Platforms</topic><topic>Quenching (cooling)</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Tyrosinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teng, Ye</creatorcontrib><creatorcontrib>Jia, Xiaofang</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Wang, Erkang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teng, Ye</au><au>Jia, Xiaofang</au><au>Li, Jing</au><au>Wang, Erkang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2015-05-05</date><risdate>2015</risdate><volume>87</volume><issue>9</issue><spage>4897</spage><epage>4902</epage><pages>4897-4902</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>In this work, a sensitive and selective ratiometric fluorescence sensing platform was built for the detection of tyrosinase (TYR) activity and dopamine (DA) using glutathione (GSH) protected gold nanoclusters (Au NCs) as probes. Upon excitation at 350 nm, Au NCs displayed an intense red emission, which could be effectively quenched by quinones. TYR, a typical polyphenol oxidase, can catalyze the oxidization of DA to o-quinone and therefore quenched the fluorescence of Au NCs. Moreover, the reaction of TYR and DA gave rise to an emission band at 400 nm, which increased in a TYR/DA-concentration-dependent manner. The ratiometric signal variations were utilized for facile, sensitive, and selective detection of TYR activity and DA. A linear range was obtained from 0.006–3.6 unit mL–1 of TYR activity, while the linear range for detection of DA was 1.0 nM to 1.0 mM. Additionally, it constructed a useful platform for TYR inhibitor screening in biomedical research.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25846058</pmid><doi>10.1021/acs.analchem.5b00468</doi><tpages>6</tpages></addata></record> |
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| subjects | Analytical chemistry Biomedical research Dopamine Dopamine - analysis Emission Fluorescence Glutathione - chemistry Gold Gold - chemistry Inhibitors Metal Nanoparticles - chemistry Monophenol Monooxygenase - analysis Monophenol Monooxygenase - metabolism Nanostructure Platforms Quenching (cooling) Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Tyrosinase |
| title | Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters |
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