NMR characterization of cooperativity: fast ligand binding coupled to slow protein dimerization

We describe a general approach for analysis of 2D NMR spectra to evaluate the cooperativity of ligand binding and protein dimerization in coupled systems. The approach is applicable to systems in which NMR spectra display separate resonances for monomeric and dimeric species but each resonance shift...

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Veröffentlicht in:	Chemical science (Cambridge) 2014-01, Vol.5 (7), p.2783-2788
Hauptverfasser:	Huma, Zil E, Ludeman, Justin P, Wilkinson, Brendan L, Payne, Richard J, Stone, Martin J
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Dimerization Dimers Ligands Monomers Nuclear magnetic resonance Proteins Spectra
Online-Zugang:	Volltext
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