Development of a doubly weighted Gerchberg-Saxton algorithm for use in multibeam imaging applications
We report on the development of a doubly weighted Gerchberg-Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluoresc...
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| Veröffentlicht in: | Optics letters 2014-04, Vol.39 (8), p.2431-2434 |
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| container_issue | 8 |
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| container_title | Optics letters |
| container_volume | 39 |
| creator | Poland, Simon P Krstajić, Nikola Knight, Robert D Henderson, Robert K Ameer-Beg, Simon M |
| description | We report on the development of a doubly weighted Gerchberg-Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluorescence signals from the sample measured with a single-photon avalanche diode (SPAD) detector array. This technique compensates for issues associated with nonuniform illumination onto the SLM, the effects due to aberrations and the variability in gain between detectors within the SPAD array to generate a uniformly illuminated multiphoton fluorescence image. We demonstrate the use of the DWGS with a number of beamlet array patterns to image muscle fibers of a 5-day-old fixed zebrafish larvae. |
| doi_str_mv | 10.1364/OL.39.002431 |
| format | Article |
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| identifier | ISSN: 0146-9592 |
| ispartof | Optics letters, 2014-04, Vol.39 (8), p.2431-2434 |
| issn | 0146-9592 1539-4794 |
| language | eng |
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| source | MEDLINE; Optica Publishing Group Journals |
| subjects | Algorithms Animals Arrays Fluorescence Gain Illumination Image detection Image Processing, Computer-Assisted - statistics & numerical data Imaging Larva - cytology Microscopy, Fluorescence, Multiphoton - instrumentation Microscopy, Fluorescence, Multiphoton - methods Microscopy, Fluorescence, Multiphoton - statistics & numerical data Muscle Fibers, Skeletal - cytology Muscles Optical Phenomena Zebrafish Zebrafish - anatomy & histology |
| title | Development of a doubly weighted Gerchberg-Saxton algorithm for use in multibeam imaging applications |
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