Enhanced Accuracy of Single-Molecule Diffusion Measurements with a Photocleavable Energy-Transfer Dyad

A photocleavable energy‐transfer dyad was synthesized, characterized, and applied to single‐molecule fluorescence microscopy. After photocleavage, a combination of independent two‐color single‐molecule tracking and analysis of single‐molecule energy‐transfer efficiencies allows the determination of...

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Veröffentlicht in:	Angewandte Chemie International Edition 2013-11, Vol.52 (47), p.12435-12438
Hauptverfasser:	Dill, Maren, Baier, Moritz C., Mecking, Stefan, Wöll, Dominik
Format:	Artikel
Sprache:	eng
Schlagworte:	Anthracenes - chemical synthesis Anthracenes - chemistry Diffusion Diffusion coefficient Evolution Fluorescence fluorescence microscopy Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fragments Förster resonance energy transfer Imides - chemistry Microscopy Microscopy, Fluorescence Perylene - chemical synthesis Perylene - chemistry photolabile groups Photolysis single-molecule spectroscopy Temporal logic Tracking Ultraviolet Rays
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creator	Dill, Maren Baier, Moritz C. Mecking, Stefan Wöll, Dominik
description	A photocleavable energy‐transfer dyad was synthesized, characterized, and applied to single‐molecule fluorescence microscopy. After photocleavage, a combination of independent two‐color single‐molecule tracking and analysis of single‐molecule energy‐transfer efficiencies allows the determination of the temporal evolution of the relative distances between both fragments from the nm to the μm scale. This gives access to a broad range of diffusion coefficients.
doi_str_mv	10.1002/anie.201303658
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subjects	Anthracenes - chemical synthesis Anthracenes - chemistry Diffusion Diffusion coefficient Evolution Fluorescence fluorescence microscopy Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fragments Förster resonance energy transfer Imides - chemistry Microscopy Microscopy, Fluorescence Perylene - chemical synthesis Perylene - chemistry photolabile groups Photolysis single-molecule spectroscopy Temporal logic Tracking Ultraviolet Rays
title	Enhanced Accuracy of Single-Molecule Diffusion Measurements with a Photocleavable Energy-Transfer Dyad
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