Screening for Protein Phosphorylation Using Nanoscale Reactions on Microdroplet Arrays

We present a novel and straightforward screening method to detect protein phosphorylations in complex protein mixtures. A proteolytic digest is separated by a conventional nanoscale liquid chromatography (nano‐LC) separation and the eluate is immediately compartmentalized into microdroplets, which a...

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Veröffentlicht in:	Angewandte Chemie International Edition 2015-01, Vol.54 (5), p.1671-1675
Hauptverfasser:	Küster, Simon K., Pabst, Martin, Zenobi, Renato, Dittrich, Petra S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - metabolism Arrays Chromatography, High Pressure Liquid droplet microfluidics Droplets Enzymes mass spectrometry microarrays Nanostructure Nanotechnology Peptides Phosphopeptides - analysis Phosphorylation Protein Array Analysis protein modifications protein phosphorylation Proteins Proteins - chemistry Proteins - metabolism Screening Software Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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