Mechanically Modulating the Photophysical Properties of Fluorescent Protein Biocomposites for Ratio- and Intensiometric Sensors
Mechanically sensitive biocomposites comprised of fluorescent proteins report stress through distinct pathways. Whereas a composite containing an enhanced yellow fluorescent protein (eYFP) exhibited hypsochromic shifts in its fluorescence emission maxima following compression, a composite containing...
Gespeichert in:
| Veröffentlicht in: | Angewandte Chemie International Edition 2014-05, Vol.53 (20), p.5088-5092 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5092 |
|---|---|
| container_issue | 20 |
| container_start_page | 5088 |
| container_title | Angewandte Chemie International Edition |
| container_volume | 53 |
| creator | Brantley, Johnathan N. Bailey, Constance B. Cannon, Joe R. Clark, Katie A. Vanden Bout, David A. Brodbelt, Jennifer S. Keatinge-Clay, Adrian T. Bielawski, Christopher W. |
| description | Mechanically sensitive biocomposites comprised of fluorescent proteins report stress through distinct pathways. Whereas a composite containing an enhanced yellow fluorescent protein (eYFP) exhibited hypsochromic shifts in its fluorescence emission maxima following compression, a composite containing a modified green fluorescent protein (GFPuv) exhibited fluorescence quenching under the action of mechanical force. These ratio‐ and intensiometric sensors demonstrate that insights garnered from disparate fields (that is, polymer mechanochemistry and biophysics) can be harnessed to guide the rational design of new classes of biomechanophore‐containing materials.
Under pressure: Poly(methyl methacrylate) composites containing either enhanced yellow fluorescent protein (eYFP) or genetically modified green fluorescent protein (GFP; see scheme) exhibited changes in photophysical properties under pressure. Whereas the eYFP composites functioned as ratiometric sensors through shifts in their fluorescence emission wavelengths, the GFP composites were intensiometric and underwent fluorescence quenching under mechanical force. |
| doi_str_mv | 10.1002/anie.201306988 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1701022288</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1523402764</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5758-3ce580eda832117dfc40f811101a3b1d17a5b8e014ba345eed59428bccf887433</originalsourceid><addsrcrecordid>eNqFkUFvEzEQRlcIREvgyhFZ4sJlg8dex95jqZo2qCkVFMHNcryzxGWzXmyvaE78dRylRIgDPdnWvPk0nlcUL4FOgVL21vQOp4wCp7NaqUfFMQgGJZeSP873ivNSKgFHxbMYbzOvFJ09LY5YJUFwoY6LX0u06xxiTddtydI3Y2eS67-RtEZyvfbJD-tt3JXJdfADhuQwEt-SeTf6gNFin3aVhK4n75y3fjP46FKGWh_IxxzmS2L6hiz6hH10foMpOEs-5YcP8XnxpDVdxBf356T4PD-7Ob0oLz-cL05PLksrpFAltygUxcYozgBk09qKtgoAKBi-ggakESuFFKqV4ZVAbERdMbWytlVK5jVMijf73CH4HyPGpDcuD991pkc_Rg2SAmUsL-hhVDBeUSZnVUZf_4Pe-jH0-SOaiRkVSlX1f6mdLVXLKvuaFNM9ZYOPMWCrh-A2Jmw1UL1zrXeu9cF1bnh1HzuuNtgc8D9yM1DvgZ-uw-0DcfrkanH2d3i573Ux4d2h14Tveia5FPrL1bmG9zC_WdZUf-W_AYf1xSE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1521897477</pqid></control><display><type>article</type><title>Mechanically Modulating the Photophysical Properties of Fluorescent Protein Biocomposites for Ratio- and Intensiometric Sensors</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Brantley, Johnathan N. ; Bailey, Constance B. ; Cannon, Joe R. ; Clark, Katie A. ; Vanden Bout, David A. ; Brodbelt, Jennifer S. ; Keatinge-Clay, Adrian T. ; Bielawski, Christopher W.</creator><creatorcontrib>Brantley, Johnathan N. ; Bailey, Constance B. ; Cannon, Joe R. ; Clark, Katie A. ; Vanden Bout, David A. ; Brodbelt, Jennifer S. ; Keatinge-Clay, Adrian T. ; Bielawski, Christopher W.</creatorcontrib><description>Mechanically sensitive biocomposites comprised of fluorescent proteins report stress through distinct pathways. Whereas a composite containing an enhanced yellow fluorescent protein (eYFP) exhibited hypsochromic shifts in its fluorescence emission maxima following compression, a composite containing a modified green fluorescent protein (GFPuv) exhibited fluorescence quenching under the action of mechanical force. These ratio‐ and intensiometric sensors demonstrate that insights garnered from disparate fields (that is, polymer mechanochemistry and biophysics) can be harnessed to guide the rational design of new classes of biomechanophore‐containing materials.
Under pressure: Poly(methyl methacrylate) composites containing either enhanced yellow fluorescent protein (eYFP) or genetically modified green fluorescent protein (GFP; see scheme) exhibited changes in photophysical properties under pressure. Whereas the eYFP composites functioned as ratiometric sensors through shifts in their fluorescence emission wavelengths, the GFP composites were intensiometric and underwent fluorescence quenching under mechanical force.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.201306988</identifier><identifier>PMID: 24715358</identifier><identifier>CODEN: ACIEAY</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>biocomposites ; Biomedical materials ; Biophysics ; Composite materials ; Compressing ; Compression ; Emission ; Fluorescence ; fluorescent protein ; Green fluorescent protein ; Green Fluorescent Proteins - chemistry ; Light ; Mechanochemistry ; Physics ; Polymers ; Polymethyl methacrylates ; Proteins ; Quenching ; Sensors ; stimulus-responsive materials ; Yellow fluorescent protein</subject><ispartof>Angewandte Chemie International Edition, 2014-05, Vol.53 (20), p.5088-5092</ispartof><rights>2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright Wiley Subscription Services, Inc. May 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5758-3ce580eda832117dfc40f811101a3b1d17a5b8e014ba345eed59428bccf887433</citedby><cites>FETCH-LOGICAL-c5758-3ce580eda832117dfc40f811101a3b1d17a5b8e014ba345eed59428bccf887433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.201306988$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.201306988$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45552,45553</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24715358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brantley, Johnathan N.</creatorcontrib><creatorcontrib>Bailey, Constance B.</creatorcontrib><creatorcontrib>Cannon, Joe R.</creatorcontrib><creatorcontrib>Clark, Katie A.</creatorcontrib><creatorcontrib>Vanden Bout, David A.</creatorcontrib><creatorcontrib>Brodbelt, Jennifer S.</creatorcontrib><creatorcontrib>Keatinge-Clay, Adrian T.</creatorcontrib><creatorcontrib>Bielawski, Christopher W.</creatorcontrib><title>Mechanically Modulating the Photophysical Properties of Fluorescent Protein Biocomposites for Ratio- and Intensiometric Sensors</title><title>Angewandte Chemie International Edition</title><addtitle>Angew. Chem. Int. Ed</addtitle><description>Mechanically sensitive biocomposites comprised of fluorescent proteins report stress through distinct pathways. Whereas a composite containing an enhanced yellow fluorescent protein (eYFP) exhibited hypsochromic shifts in its fluorescence emission maxima following compression, a composite containing a modified green fluorescent protein (GFPuv) exhibited fluorescence quenching under the action of mechanical force. These ratio‐ and intensiometric sensors demonstrate that insights garnered from disparate fields (that is, polymer mechanochemistry and biophysics) can be harnessed to guide the rational design of new classes of biomechanophore‐containing materials.
Under pressure: Poly(methyl methacrylate) composites containing either enhanced yellow fluorescent protein (eYFP) or genetically modified green fluorescent protein (GFP; see scheme) exhibited changes in photophysical properties under pressure. Whereas the eYFP composites functioned as ratiometric sensors through shifts in their fluorescence emission wavelengths, the GFP composites were intensiometric and underwent fluorescence quenching under mechanical force.</description><subject>biocomposites</subject><subject>Biomedical materials</subject><subject>Biophysics</subject><subject>Composite materials</subject><subject>Compressing</subject><subject>Compression</subject><subject>Emission</subject><subject>Fluorescence</subject><subject>fluorescent protein</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Light</subject><subject>Mechanochemistry</subject><subject>Physics</subject><subject>Polymers</subject><subject>Polymethyl methacrylates</subject><subject>Proteins</subject><subject>Quenching</subject><subject>Sensors</subject><subject>stimulus-responsive materials</subject><subject>Yellow fluorescent protein</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvEzEQRlcIREvgyhFZ4sJlg8dex95jqZo2qCkVFMHNcryzxGWzXmyvaE78dRylRIgDPdnWvPk0nlcUL4FOgVL21vQOp4wCp7NaqUfFMQgGJZeSP873ivNSKgFHxbMYbzOvFJ09LY5YJUFwoY6LX0u06xxiTddtydI3Y2eS67-RtEZyvfbJD-tt3JXJdfADhuQwEt-SeTf6gNFin3aVhK4n75y3fjP46FKGWh_IxxzmS2L6hiz6hH10foMpOEs-5YcP8XnxpDVdxBf356T4PD-7Ob0oLz-cL05PLksrpFAltygUxcYozgBk09qKtgoAKBi-ggakESuFFKqV4ZVAbERdMbWytlVK5jVMijf73CH4HyPGpDcuD991pkc_Rg2SAmUsL-hhVDBeUSZnVUZf_4Pe-jH0-SOaiRkVSlX1f6mdLVXLKvuaFNM9ZYOPMWCrh-A2Jmw1UL1zrXeu9cF1bnh1HzuuNtgc8D9yM1DvgZ-uw-0DcfrkanH2d3i573Ux4d2h14Tveia5FPrL1bmG9zC_WdZUf-W_AYf1xSE</recordid><startdate>20140512</startdate><enddate>20140512</enddate><creator>Brantley, Johnathan N.</creator><creator>Bailey, Constance B.</creator><creator>Cannon, Joe R.</creator><creator>Clark, Katie A.</creator><creator>Vanden Bout, David A.</creator><creator>Brodbelt, Jennifer S.</creator><creator>Keatinge-Clay, Adrian T.</creator><creator>Bielawski, Christopher W.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20140512</creationdate><title>Mechanically Modulating the Photophysical Properties of Fluorescent Protein Biocomposites for Ratio- and Intensiometric Sensors</title><author>Brantley, Johnathan N. ; Bailey, Constance B. ; Cannon, Joe R. ; Clark, Katie A. ; Vanden Bout, David A. ; Brodbelt, Jennifer S. ; Keatinge-Clay, Adrian T. ; Bielawski, Christopher W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5758-3ce580eda832117dfc40f811101a3b1d17a5b8e014ba345eed59428bccf887433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>biocomposites</topic><topic>Biomedical materials</topic><topic>Biophysics</topic><topic>Composite materials</topic><topic>Compressing</topic><topic>Compression</topic><topic>Emission</topic><topic>Fluorescence</topic><topic>fluorescent protein</topic><topic>Green fluorescent protein</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Light</topic><topic>Mechanochemistry</topic><topic>Physics</topic><topic>Polymers</topic><topic>Polymethyl methacrylates</topic><topic>Proteins</topic><topic>Quenching</topic><topic>Sensors</topic><topic>stimulus-responsive materials</topic><topic>Yellow fluorescent protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brantley, Johnathan N.</creatorcontrib><creatorcontrib>Bailey, Constance B.</creatorcontrib><creatorcontrib>Cannon, Joe R.</creatorcontrib><creatorcontrib>Clark, Katie A.</creatorcontrib><creatorcontrib>Vanden Bout, David A.</creatorcontrib><creatorcontrib>Brodbelt, Jennifer S.</creatorcontrib><creatorcontrib>Keatinge-Clay, Adrian T.</creatorcontrib><creatorcontrib>Bielawski, Christopher W.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brantley, Johnathan N.</au><au>Bailey, Constance B.</au><au>Cannon, Joe R.</au><au>Clark, Katie A.</au><au>Vanden Bout, David A.</au><au>Brodbelt, Jennifer S.</au><au>Keatinge-Clay, Adrian T.</au><au>Bielawski, Christopher W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanically Modulating the Photophysical Properties of Fluorescent Protein Biocomposites for Ratio- and Intensiometric Sensors</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew. Chem. Int. Ed</addtitle><date>2014-05-12</date><risdate>2014</risdate><volume>53</volume><issue>20</issue><spage>5088</spage><epage>5092</epage><pages>5088-5092</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><coden>ACIEAY</coden><abstract>Mechanically sensitive biocomposites comprised of fluorescent proteins report stress through distinct pathways. Whereas a composite containing an enhanced yellow fluorescent protein (eYFP) exhibited hypsochromic shifts in its fluorescence emission maxima following compression, a composite containing a modified green fluorescent protein (GFPuv) exhibited fluorescence quenching under the action of mechanical force. These ratio‐ and intensiometric sensors demonstrate that insights garnered from disparate fields (that is, polymer mechanochemistry and biophysics) can be harnessed to guide the rational design of new classes of biomechanophore‐containing materials.
Under pressure: Poly(methyl methacrylate) composites containing either enhanced yellow fluorescent protein (eYFP) or genetically modified green fluorescent protein (GFP; see scheme) exhibited changes in photophysical properties under pressure. Whereas the eYFP composites functioned as ratiometric sensors through shifts in their fluorescence emission wavelengths, the GFP composites were intensiometric and underwent fluorescence quenching under mechanical force.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>24715358</pmid><doi>10.1002/anie.201306988</doi><tpages>5</tpages><edition>International ed. in English</edition></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1433-7851 |
| ispartof | Angewandte Chemie International Edition, 2014-05, Vol.53 (20), p.5088-5092 |
| issn | 1433-7851 1521-3773 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1701022288 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | biocomposites Biomedical materials Biophysics Composite materials Compressing Compression Emission Fluorescence fluorescent protein Green fluorescent protein Green Fluorescent Proteins - chemistry Light Mechanochemistry Physics Polymers Polymethyl methacrylates Proteins Quenching Sensors stimulus-responsive materials Yellow fluorescent protein |
| title | Mechanically Modulating the Photophysical Properties of Fluorescent Protein Biocomposites for Ratio- and Intensiometric Sensors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T09%3A49%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mechanically%20Modulating%20the%20Photophysical%20Properties%20of%20Fluorescent%20Protein%20Biocomposites%20for%20Ratio-%20and%20Intensiometric%20Sensors&rft.jtitle=Angewandte%20Chemie%20International%20Edition&rft.au=Brantley,%20Johnathan%20N.&rft.date=2014-05-12&rft.volume=53&rft.issue=20&rft.spage=5088&rft.epage=5092&rft.pages=5088-5092&rft.issn=1433-7851&rft.eissn=1521-3773&rft.coden=ACIEAY&rft_id=info:doi/10.1002/anie.201306988&rft_dat=%3Cproquest_cross%3E1523402764%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1521897477&rft_id=info:pmid/24715358&rfr_iscdi=true |