Deciphering a Nanocarbon-Based Artificial Peroxidase: Chemical Identification of the Catalytically Active and Substrate-Binding Sites on Graphene Quantum Dots

The design and construction of efficient artificial enzymes is highly desirable. Recent studies have demonstrated that a series of carbon nanomaterials possess intrinsic peroxidase activity. Among them, graphene quantum dots (GQDs) have a high enzymatic activity. However, the catalytic mechanism remains unclear. Therefore, in this report, we chose to decipher their peroxidase activity. By selectively deactivating the ketonic carbonyl, carboxylic, or hydroxy groups and investigating the catalytic activities of these GQD derivatives, we obtained evidence that the CO groups were the catalytically active sites, whereas the OCO groups acted as substrate‐binding sites, and COH groups can inhibit the activity. These results were corroborated by theoretical studies. This work should not only enhance our understanding of nanocarbon‐based artificial enzymes, but also facilitate the design and construction of other types of target‐specific artificial enzymes. The peroxidase‐like activity of graphene quantum dots (GQDs) is studied by means of selective deactivation of the different functional groups on GQDs. Experimental results and theoretical calculations demonstrate that ketone groups are the catalytically active sites whereas carboxylic groups act as substrate‐binding sites, and hydroxy groups can decrease the catalytic activity.