Assessment of STR Typing Success Rate in Soft Tissues from Putrefied Bodies Based on a Quantitative Grading System for Putrefaction
To date, there is no systematic investigation of the association of short tandem repeat (STR) typing success rate in soft tissues with different signs of putrefaction. Herein, putrefaction was rated using a newly developed 19‐parameter system in soft tissues from a collective of 68 decaying bodies,...
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| Veröffentlicht in: | Journal of forensic sciences 2015-07, Vol.60 (4), p.1016-1021 |
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| container_title | Journal of forensic sciences |
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| creator | Courts, Cornelius Sauer, Eva Hofmann, Yaiza Madea, Burkhard Schyma, Christian |
| description | To date, there is no systematic investigation of the association of short tandem repeat (STR) typing success rate in soft tissues with different signs of putrefaction. Herein, putrefaction was rated using a newly developed 19‐parameter system in soft tissues from a collective of 68 decaying bodies, and DNA yield was determined in 408 samples. DNA integrity was rated using a self‐devised pentaplex PCR generating an “integrity score” (Si). STR typing success rate was then assessed for selected cases. DNA yield and Si differed significantly between tissues with kidney on average exhibiting the highest Si values. Statistical analysis revealed that nine parameters were significantly and positively correlated with Si. The observed values for each of these nine parameters were summed up to generate a putrefaction score (Sp) for each sample. Our results show that STR typing success rate can be predicted based on Sp before expensive multiplex STR profiling is performed. |
| doi_str_mv | 10.1111/1556-4029.12746 |
| format | Article |
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Herein, putrefaction was rated using a newly developed 19‐parameter system in soft tissues from a collective of 68 decaying bodies, and DNA yield was determined in 408 samples. DNA integrity was rated using a self‐devised pentaplex PCR generating an “integrity score” (Si). STR typing success rate was then assessed for selected cases. DNA yield and Si differed significantly between tissues with kidney on average exhibiting the highest Si values. Statistical analysis revealed that nine parameters were significantly and positively correlated with Si. The observed values for each of these nine parameters were summed up to generate a putrefaction score (Sp) for each sample. 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Herein, putrefaction was rated using a newly developed 19‐parameter system in soft tissues from a collective of 68 decaying bodies, and DNA yield was determined in 408 samples. DNA integrity was rated using a self‐devised pentaplex PCR generating an “integrity score” (Si). STR typing success rate was then assessed for selected cases. DNA yield and Si differed significantly between tissues with kidney on average exhibiting the highest Si values. Statistical analysis revealed that nine parameters were significantly and positively correlated with Si. The observed values for each of these nine parameters were summed up to generate a putrefaction score (Sp) for each sample. Our results show that STR typing success rate can be predicted based on Sp before expensive multiplex STR profiling is performed.</description><subject>Aorta - chemistry</subject><subject>Brain Chemistry</subject><subject>Deoxyribonucleic acid</subject><subject>disaster victim identification</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA Fingerprinting</subject><subject>DNA integrity</subject><subject>DNA profiling</subject><subject>Female</subject><subject>forensic genetics</subject><subject>forensic science</subject><subject>Forensic sciences</subject><subject>Humans</subject><subject>Kidney - chemistry</subject><subject>Liver - chemistry</subject><subject>Lung - chemistry</subject><subject>Male</subject><subject>Microsatellite Repeats</subject><subject>Polymerase Chain Reaction</subject><subject>Postmortem Changes</subject><subject>Psoas Muscles - chemistry</subject><subject>putrefaction</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9PFDEUxxujkQU5ezNNvHAZaKfTdnoEIouGiLBLTLg0nf4wxZ3p2nbAPfuP22WXPXixl6avn-83770vAO8xOsblnGBKWdWgWhzjmjfsFZjsKq_BBKG6rjAW7R7YT-kBIcQww2_BXk1b1HJST8Cf05RsSr0dMgwOzua3cL5a-uEHnI1alx94q7KFfoCz4DKc-5RGm6CLoYffxhyt89bAs2B8qZ6pVB5hgArejGrIPqvsHy2cRmWeLVcp2x66ELdapbMPwzvwxqlFsofb-wDcXXyan19WV9fTz-enV5VukChDEdRpwlinNBPO1W3TGME6R4RSRgnuFO0Q1lw3HTMYN9QYpnCNCUedKwA5AEcb32UMv8oUWfY-abtYqMGGMUnMy4Jaxkhb0I__oA9hjEPpTmImBC-mvCnUyYbSMaRU5pHL6HsVVxIjuc5HrtOQ6zTkcz5F8WHrO3a9NTv-JZAC0A3w5Bd29T8_-eXi-sW42uh8WfHvnU7Fn5Jxwqn8_nUq6f0NvSRUyHvyF6mZqYA</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Courts, Cornelius</creator><creator>Sauer, Eva</creator><creator>Hofmann, Yaiza</creator><creator>Madea, Burkhard</creator><creator>Schyma, Christian</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Assessment of STR Typing Success Rate in Soft Tissues from Putrefied Bodies Based on a Quantitative Grading System for Putrefaction</title><author>Courts, Cornelius ; Sauer, Eva ; Hofmann, Yaiza ; Madea, Burkhard ; Schyma, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4096-430bc366bac69ff2844d96bf39aada97fa5b01c7c4b6d1145dd6a121370bfda93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aorta - chemistry</topic><topic>Brain Chemistry</topic><topic>Deoxyribonucleic acid</topic><topic>disaster victim identification</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA Fingerprinting</topic><topic>DNA integrity</topic><topic>DNA profiling</topic><topic>Female</topic><topic>forensic genetics</topic><topic>forensic science</topic><topic>Forensic sciences</topic><topic>Humans</topic><topic>Kidney - chemistry</topic><topic>Liver - chemistry</topic><topic>Lung - chemistry</topic><topic>Male</topic><topic>Microsatellite Repeats</topic><topic>Polymerase Chain Reaction</topic><topic>Postmortem Changes</topic><topic>Psoas Muscles - chemistry</topic><topic>putrefaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Courts, Cornelius</creatorcontrib><creatorcontrib>Sauer, Eva</creatorcontrib><creatorcontrib>Hofmann, Yaiza</creatorcontrib><creatorcontrib>Madea, Burkhard</creatorcontrib><creatorcontrib>Schyma, Christian</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Courts, Cornelius</au><au>Sauer, Eva</au><au>Hofmann, Yaiza</au><au>Madea, Burkhard</au><au>Schyma, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of STR Typing Success Rate in Soft Tissues from Putrefied Bodies Based on a Quantitative Grading System for Putrefaction</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2015-07</date><risdate>2015</risdate><volume>60</volume><issue>4</issue><spage>1016</spage><epage>1021</epage><pages>1016-1021</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><coden>JFSCAS</coden><abstract>To date, there is no systematic investigation of the association of short tandem repeat (STR) typing success rate in soft tissues with different signs of putrefaction. Herein, putrefaction was rated using a newly developed 19‐parameter system in soft tissues from a collective of 68 decaying bodies, and DNA yield was determined in 408 samples. DNA integrity was rated using a self‐devised pentaplex PCR generating an “integrity score” (Si). STR typing success rate was then assessed for selected cases. DNA yield and Si differed significantly between tissues with kidney on average exhibiting the highest Si values. Statistical analysis revealed that nine parameters were significantly and positively correlated with Si. The observed values for each of these nine parameters were summed up to generate a putrefaction score (Sp) for each sample. Our results show that STR typing success rate can be predicted based on Sp before expensive multiplex STR profiling is performed.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25808732</pmid><doi>10.1111/1556-4029.12746</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of forensic sciences, 2015-07, Vol.60 (4), p.1016-1021 |
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| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Aorta - chemistry Brain Chemistry Deoxyribonucleic acid disaster victim identification DNA DNA - analysis DNA Fingerprinting DNA integrity DNA profiling Female forensic genetics forensic science Forensic sciences Humans Kidney - chemistry Liver - chemistry Lung - chemistry Male Microsatellite Repeats Polymerase Chain Reaction Postmortem Changes Psoas Muscles - chemistry putrefaction |
| title | Assessment of STR Typing Success Rate in Soft Tissues from Putrefied Bodies Based on a Quantitative Grading System for Putrefaction |
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