Molecular genetics and transport analysis of the copper‐resistance determinant (pco) from Escherichia coli plasmid pRJ1004
The copper‐resistance determinant (pco) of Escherichia coli plasmid pRJ1004 was cloned and sequenced. Tn1000 transposon mutagenesis identified four complementation groups, mutations in any of which eliminated copper resistance. DNA sequence analysis showed that the four complementation groups contai...
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| Veröffentlicht in: | Molecular microbiology 1995-09, Vol.17 (6), p.1153-1166 |
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| creator | Brown, Nigel L. Barrett, Siobhan R. Camakaris, James Lee, Barry T.O. Rouch, Duncan A. |
| description | The copper‐resistance determinant (pco) of Escherichia coli plasmid pRJ1004 was cloned and sequenced. Tn1000 transposon mutagenesis identified four complementation groups, mutations in any of which eliminated copper resistance. DNA sequence analysis showed that the four complementation groups contained six open reading frames, designated pcoABCDRS. The protein product sequences derived from the nucleotide sequence show close homology between this copper‐resistance system and the cop system of a plasmid pPT23D of Pseudomonas syringae pv. tomato. The PcoR and PcoS protein sequences show homology to the family of two‐component sensor/responder phosphokinase regulatory systems. A seventh reading frame (pcoE) was identified from DNA sequence data, and lies downstream of a copper‐regulated promoter. Transport assays with 64Cu(II) showed that the resistant cells containing the plasmid had reduced copper accumulation during the log phase of growth, while increased accumulation had previously been observed during stationary phase. Chromosomal mutants defective in cellular copper management were obtained and characterized. In two of these mutants pco resistance was rendered totally inactive, whilst in another two mutants pco complemented the defective genes. These data indicate that plasmid‐borne copper resistance in E. coli is linked with chromosomal systems for copper management. |
| doi_str_mv | 10.1111/j.1365-2958.1995.mmi_17061153.x |
| format | Article |
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Tn1000 transposon mutagenesis identified four complementation groups, mutations in any of which eliminated copper resistance. DNA sequence analysis showed that the four complementation groups contained six open reading frames, designated pcoABCDRS. The protein product sequences derived from the nucleotide sequence show close homology between this copper‐resistance system and the cop system of a plasmid pPT23D of Pseudomonas syringae pv. tomato. The PcoR and PcoS protein sequences show homology to the family of two‐component sensor/responder phosphokinase regulatory systems. A seventh reading frame (pcoE) was identified from DNA sequence data, and lies downstream of a copper‐regulated promoter. Transport assays with 64Cu(II) showed that the resistant cells containing the plasmid had reduced copper accumulation during the log phase of growth, while increased accumulation had previously been observed during stationary phase. Chromosomal mutants defective in cellular copper management were obtained and characterized. In two of these mutants pco resistance was rendered totally inactive, whilst in another two mutants pco complemented the defective genes. These data indicate that plasmid‐borne copper resistance in E. coli is linked with chromosomal systems for copper management.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1995.mmi_17061153.x</identifier><identifier>PMID: 8594334</identifier><language>eng</language><publisher>Osney Mead, Oxford OX2 0EL, UK: Blackwell Scientific Publications</publisher><subject>Amino Acid Sequence ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Outer Membrane Proteins - physiology ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Biological Transport, Active ; Carrier Proteins - genetics ; Carrier Proteins - physiology ; Cation Transport Proteins ; Chromosomes, Bacterial ; Cloning, Molecular ; Consensus Sequence ; Copper - metabolism ; Copper - pharmacology ; DNA Transposable Elements ; DNA, Bacterial - genetics ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - physiology ; Drug Resistance, Microbial - genetics ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli Proteins ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genes, Regulator ; Genetic Complementation Test ; Models, Biological ; Molecular Sequence Data ; Mutagenesis, Insertional ; Operon ; Pseudomonas - genetics ; R Factors - genetics ; Recombinant Fusion Proteins - metabolism ; Sequence Alignment ; Sequence Homology, Amino Acid ; Trans-Activators - genetics ; Trans-Activators - physiology ; Transferases ; Xanthomonas campestris - genetics</subject><ispartof>Molecular microbiology, 1995-09, Vol.17 (6), p.1153-1166</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4863-906f7ae0d4514cf563b67b3d0ea83eb9303cd6c776091195e5022eb861004c903</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1995.mmi_17061153.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1995.mmi_17061153.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8594334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, Nigel L.</creatorcontrib><creatorcontrib>Barrett, Siobhan R.</creatorcontrib><creatorcontrib>Camakaris, James</creatorcontrib><creatorcontrib>Lee, Barry T.O.</creatorcontrib><creatorcontrib>Rouch, Duncan A.</creatorcontrib><title>Molecular genetics and transport analysis of the copper‐resistance determinant (pco) from Escherichia coli plasmid pRJ1004</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>The copper‐resistance determinant (pco) of Escherichia coli plasmid pRJ1004 was cloned and sequenced. Tn1000 transposon mutagenesis identified four complementation groups, mutations in any of which eliminated copper resistance. DNA sequence analysis showed that the four complementation groups contained six open reading frames, designated pcoABCDRS. The protein product sequences derived from the nucleotide sequence show close homology between this copper‐resistance system and the cop system of a plasmid pPT23D of Pseudomonas syringae pv. tomato. The PcoR and PcoS protein sequences show homology to the family of two‐component sensor/responder phosphokinase regulatory systems. A seventh reading frame (pcoE) was identified from DNA sequence data, and lies downstream of a copper‐regulated promoter. Transport assays with 64Cu(II) showed that the resistant cells containing the plasmid had reduced copper accumulation during the log phase of growth, while increased accumulation had previously been observed during stationary phase. Chromosomal mutants defective in cellular copper management were obtained and characterized. In two of these mutants pco resistance was rendered totally inactive, whilst in another two mutants pco complemented the defective genes. These data indicate that plasmid‐borne copper resistance in E. coli is linked with chromosomal systems for copper management.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Outer Membrane Proteins - physiology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Biological Transport, Active</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - physiology</subject><subject>Cation Transport Proteins</subject><subject>Chromosomes, Bacterial</subject><subject>Cloning, Molecular</subject><subject>Consensus Sequence</subject><subject>Copper - metabolism</subject><subject>Copper - pharmacology</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Genes, Regulator</subject><subject>Genetic Complementation Test</subject><subject>Models, Biological</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>Operon</subject><subject>Pseudomonas - genetics</subject><subject>R Factors - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - physiology</subject><subject>Transferases</subject><subject>Xanthomonas campestris - genetics</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMFu1DAQhq0K1C6FR6jkU4FD0vE6duITQlWhRV1VQiBxsxxn0vXKiVM7K7oSBx6BZ-RJSLRLxZW5jGb-mf-XPkJeM8jZVBebnHEpsqUSVc6UEnnXOc1KkIwJnj8ekcWT_owsQAnIeLX8dkJepLQBYBwkPybHlVAF58WC_FgFj3brTaT32OPobKKmb-gYTZ-GEMdpMn6XXKKhpeMaqQ3DgPH3z18Rp-1oeou0wRFj53rTj_TNYMNb2sbQ0atk1xidXTszvXlHB29S5xo6fP7EAIqX5HlrfMJXh35Kvn64-nJ5nd3efby5fH-b2aKSPFMg29IgNIVghW2F5LUsa94AmopjrThw20hblhIUY0qggOUS60rOGVYBPyXne98hhoctplF3Lln03vQYtmnmB6Io58N3-0MbQ0oRWz1E15m40wz0zF9v9MxXz3z1zF__y18_Tg5nh6ht3WHz9H8APunXe_2787j7X3u9Wt38nfgfC-Gazg</recordid><startdate>199509</startdate><enddate>199509</enddate><creator>Brown, Nigel L.</creator><creator>Barrett, Siobhan R.</creator><creator>Camakaris, James</creator><creator>Lee, Barry T.O.</creator><creator>Rouch, Duncan A.</creator><general>Blackwell Scientific Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199509</creationdate><title>Molecular genetics and transport analysis of the copper‐resistance determinant (pco) from Escherichia coli plasmid pRJ1004</title><author>Brown, Nigel L. ; Barrett, Siobhan R. ; Camakaris, James ; Lee, Barry T.O. ; Rouch, Duncan A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4863-906f7ae0d4514cf563b67b3d0ea83eb9303cd6c776091195e5022eb861004c903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Outer Membrane Proteins - physiology</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Biological Transport, Active</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - physiology</topic><topic>Cation Transport Proteins</topic><topic>Chromosomes, Bacterial</topic><topic>Cloning, Molecular</topic><topic>Consensus Sequence</topic><topic>Copper - metabolism</topic><topic>Copper - pharmacology</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Proteins</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Genes, Regulator</topic><topic>Genetic Complementation Test</topic><topic>Models, Biological</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>Operon</topic><topic>Pseudomonas - genetics</topic><topic>R Factors - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - physiology</topic><topic>Transferases</topic><topic>Xanthomonas campestris - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, Nigel L.</creatorcontrib><creatorcontrib>Barrett, Siobhan R.</creatorcontrib><creatorcontrib>Camakaris, James</creatorcontrib><creatorcontrib>Lee, Barry T.O.</creatorcontrib><creatorcontrib>Rouch, Duncan A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Nigel L.</au><au>Barrett, Siobhan R.</au><au>Camakaris, James</au><au>Lee, Barry T.O.</au><au>Rouch, Duncan A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular genetics and transport analysis of the copper‐resistance determinant (pco) from Escherichia coli plasmid pRJ1004</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1995-09</date><risdate>1995</risdate><volume>17</volume><issue>6</issue><spage>1153</spage><epage>1166</epage><pages>1153-1166</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>The copper‐resistance determinant (pco) of Escherichia coli plasmid pRJ1004 was cloned and sequenced. Tn1000 transposon mutagenesis identified four complementation groups, mutations in any of which eliminated copper resistance. DNA sequence analysis showed that the four complementation groups contained six open reading frames, designated pcoABCDRS. The protein product sequences derived from the nucleotide sequence show close homology between this copper‐resistance system and the cop system of a plasmid pPT23D of Pseudomonas syringae pv. tomato. The PcoR and PcoS protein sequences show homology to the family of two‐component sensor/responder phosphokinase regulatory systems. A seventh reading frame (pcoE) was identified from DNA sequence data, and lies downstream of a copper‐regulated promoter. Transport assays with 64Cu(II) showed that the resistant cells containing the plasmid had reduced copper accumulation during the log phase of growth, while increased accumulation had previously been observed during stationary phase. Chromosomal mutants defective in cellular copper management were obtained and characterized. In two of these mutants pco resistance was rendered totally inactive, whilst in another two mutants pco complemented the defective genes. These data indicate that plasmid‐borne copper resistance in E. coli is linked with chromosomal systems for copper management.</abstract><cop>Osney Mead, Oxford OX2 0EL, UK</cop><pub>Blackwell Scientific Publications</pub><pmid>8594334</pmid><doi>10.1111/j.1365-2958.1995.mmi_17061153.x</doi><tpages>14</tpages></addata></record> |
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| ispartof | Molecular microbiology, 1995-09, Vol.17 (6), p.1153-1166 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acid Sequence Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - physiology Bacterial Proteins - genetics Bacterial Proteins - physiology Biological Transport, Active Carrier Proteins - genetics Carrier Proteins - physiology Cation Transport Proteins Chromosomes, Bacterial Cloning, Molecular Consensus Sequence Copper - metabolism Copper - pharmacology DNA Transposable Elements DNA, Bacterial - genetics DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology Drug Resistance, Microbial - genetics Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Genetic Complementation Test Models, Biological Molecular Sequence Data Mutagenesis, Insertional Operon Pseudomonas - genetics R Factors - genetics Recombinant Fusion Proteins - metabolism Sequence Alignment Sequence Homology, Amino Acid Trans-Activators - genetics Trans-Activators - physiology Transferases Xanthomonas campestris - genetics |
| title | Molecular genetics and transport analysis of the copper‐resistance determinant (pco) from Escherichia coli plasmid pRJ1004 |
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