The Environment of [2Fe-2S] Clusters in Ferredoxins: The Role of Residue 45 Probed by Site-Directed Mutagenesis

The biochemical and biophysical properties of the Ala45Ser mutant of the [2Fe-2S] ferredoxin from vegetative cells of the cyanobacterium Anabaena sp. 7120 are described. This novel protein, which incorporates the residue present in many higher plant ferredoxins into the analogous position of a typic...

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