Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals
Abstract Introduction The present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model. Methods Cells of E. faecalis were introduced into the dentinal tubules by cen...
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| Veröffentlicht in: | Journal of endodontics 2015-08, Vol.41 (8), p.1294-1298 |
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| creator | Du, Tianfeng, PhD Wang, Zhejun, PhD Shen, Ya, PhD Ma, Jingzhi, PhD Cao, Yingguang, PhD Haapasalo, Markus, Dr Odont |
| description | Abstract Introduction The present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model. Methods Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion for 3 weeks. The biofilms in dentin were first subjected to 5% NaOCl or sterile water for 10 minutes followed by an equal thickness of AH Plus (Dentsply International Inc, York, PA), Endosequence BC Sealer (Brasseler USA, Savannah, GA), or MTA Fillapex (Angelus Indústria de Produtos Odontológicos S/A, Londrina, Brazil) placed on the root canal wall of the dentin specimens for 7, 30, and 60 days. Gutta-percha and water were used in a similar manner as controls. The proportions of dead and live bacteria inside the dentinal tubules were assessed by confocal laser scanning microscopy and viability staining. Results The combined use of NaOCl and sealers (30 and 60 days) killed significantly more bacteria than NaOCl or sealers alone ( P |
| doi_str_mv | 10.1016/j.joen.2015.04.023 |
| format | Article |
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Methods Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion for 3 weeks. The biofilms in dentin were first subjected to 5% NaOCl or sterile water for 10 minutes followed by an equal thickness of AH Plus (Dentsply International Inc, York, PA), Endosequence BC Sealer (Brasseler USA, Savannah, GA), or MTA Fillapex (Angelus Indústria de Produtos Odontológicos S/A, Londrina, Brazil) placed on the root canal wall of the dentin specimens for 7, 30, and 60 days. Gutta-percha and water were used in a similar manner as controls. The proportions of dead and live bacteria inside the dentinal tubules were assessed by confocal laser scanning microscopy and viability staining. Results The combined use of NaOCl and sealers (30 and 60 days) killed significantly more bacteria than NaOCl or sealers alone ( P < .05). NaOCl + MTA Fillapex was the most effective antibacterial combination by killing 83% bacteria in dentin tubules in 60 days. Thirty and 60 days of exposure to the sealers resulted in significantly more dead bacteria in dentin biofilms than 7-day exposures ( P < .05). Conclusions The placement of root canal sealer after NaOCl treatment enhanced antibacterial effects against E. faecalis in the dentinal tubules. Little additional effect was obtained after 30 days of exposure to sealers.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2015.04.023</identifier><identifier>PMID: 26092772</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anti-Bacterial Agents - pharmacology ; Biofilms - drug effects ; Confocal laser scanning microscopy ; dentin ; Dentin - drug effects ; Dentin - microbiology ; Dentin - pathology ; Dentistry ; Drug Therapy, Combination ; Endocrinology & Metabolism ; Enterococcus faecalis ; Enterococcus faecalis - drug effects ; Epoxy Resins - pharmacology ; Gram-Positive Bacterial Infections - drug therapy ; Gram-Positive Bacterial Infections - pathology ; Humans ; Microscopy, Confocal ; Root Canal Filling Materials - pharmacology ; sealer ; sodium hypochlorite ; Sodium Hypochlorite - pharmacology ; Time Factors</subject><ispartof>Journal of endodontics, 2015-08, Vol.41 (8), p.1294-1298</ispartof><rights>American Association of Endodontists</rights><rights>2015 American Association of Endodontists</rights><rights>Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-9903381e52febcc81d5f4f0dea8a1c75909e70a7ce845a8fdbf2ee0d20069aef3</citedby><cites>FETCH-LOGICAL-c481t-9903381e52febcc81d5f4f0dea8a1c75909e70a7ce845a8fdbf2ee0d20069aef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0099239915004112$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26092772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Tianfeng, PhD</creatorcontrib><creatorcontrib>Wang, Zhejun, PhD</creatorcontrib><creatorcontrib>Shen, Ya, PhD</creatorcontrib><creatorcontrib>Ma, Jingzhi, PhD</creatorcontrib><creatorcontrib>Cao, Yingguang, PhD</creatorcontrib><creatorcontrib>Haapasalo, Markus, Dr Odont</creatorcontrib><title>Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract Introduction The present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model. Methods Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion for 3 weeks. The biofilms in dentin were first subjected to 5% NaOCl or sterile water for 10 minutes followed by an equal thickness of AH Plus (Dentsply International Inc, York, PA), Endosequence BC Sealer (Brasseler USA, Savannah, GA), or MTA Fillapex (Angelus Indústria de Produtos Odontológicos S/A, Londrina, Brazil) placed on the root canal wall of the dentin specimens for 7, 30, and 60 days. Gutta-percha and water were used in a similar manner as controls. The proportions of dead and live bacteria inside the dentinal tubules were assessed by confocal laser scanning microscopy and viability staining. Results The combined use of NaOCl and sealers (30 and 60 days) killed significantly more bacteria than NaOCl or sealers alone ( P < .05). NaOCl + MTA Fillapex was the most effective antibacterial combination by killing 83% bacteria in dentin tubules in 60 days. Thirty and 60 days of exposure to the sealers resulted in significantly more dead bacteria in dentin biofilms than 7-day exposures ( P < .05). Conclusions The placement of root canal sealer after NaOCl treatment enhanced antibacterial effects against E. faecalis in the dentinal tubules. Little additional effect was obtained after 30 days of exposure to sealers.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biofilms - drug effects</subject><subject>Confocal laser scanning microscopy</subject><subject>dentin</subject><subject>Dentin - drug effects</subject><subject>Dentin - microbiology</subject><subject>Dentin - pathology</subject><subject>Dentistry</subject><subject>Drug Therapy, Combination</subject><subject>Endocrinology & Metabolism</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - drug effects</subject><subject>Epoxy Resins - pharmacology</subject><subject>Gram-Positive Bacterial Infections - drug therapy</subject><subject>Gram-Positive Bacterial Infections - pathology</subject><subject>Humans</subject><subject>Microscopy, Confocal</subject><subject>Root Canal Filling Materials - pharmacology</subject><subject>sealer</subject><subject>sodium hypochlorite</subject><subject>Sodium Hypochlorite - pharmacology</subject><subject>Time Factors</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kbGO1DAQhiME4vYOXoACuaRJGNvJJpYQ0rHscUgnIbFQW449BofEXuwEaWteHIc9KCiopvn-fzTfFMUzChUFun05VENAXzGgTQV1BYw_KDa0a7uSN039sNgACFEyLsRFcZnSAEBbztvHxQXbgmBtyzbFz12YeufRkGs_u17pGaNTI9lbi3omwZJDMG6ZyO3pGPTXMUQ3I1HekI8hzGSnfIYPqEaMiagvyvk0k73PLUEHrZdErEKtRpfIGxesG6dEnCdvMW_z53h6UjyyeeDT-3lVfL7Zf9rdlncf3r3fXd-Vuu7oXAoBnHcUG2ax17qjprG1BYOqU1S3jQCBLahWY1c3qrOmtwwRDAPYCoWWXxUvzr3HGL4vmGY5uaRxHJXHsCRJ22wos4xnlJ1RHUNKEa08RjepeJIU5CpfDnKVL1f5EmoJv0PP7_uXfkLzN_LHdgZenQHMV_5wGGXSDr1G42K2LU1w_-9__U9cj867bPcbnjANYYmrTkllYhLkYX3_-n3aANSUMv4L7uqsxA</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Du, Tianfeng, PhD</creator><creator>Wang, Zhejun, PhD</creator><creator>Shen, Ya, PhD</creator><creator>Ma, Jingzhi, PhD</creator><creator>Cao, Yingguang, PhD</creator><creator>Haapasalo, Markus, Dr Odont</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150801</creationdate><title>Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals</title><author>Du, Tianfeng, PhD ; Wang, Zhejun, PhD ; Shen, Ya, PhD ; Ma, Jingzhi, PhD ; Cao, Yingguang, PhD ; Haapasalo, Markus, Dr Odont</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-9903381e52febcc81d5f4f0dea8a1c75909e70a7ce845a8fdbf2ee0d20069aef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biofilms - drug effects</topic><topic>Confocal laser scanning microscopy</topic><topic>dentin</topic><topic>Dentin - drug effects</topic><topic>Dentin - microbiology</topic><topic>Dentin - pathology</topic><topic>Dentistry</topic><topic>Drug Therapy, Combination</topic><topic>Endocrinology & Metabolism</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - drug effects</topic><topic>Epoxy Resins - pharmacology</topic><topic>Gram-Positive Bacterial Infections - drug therapy</topic><topic>Gram-Positive Bacterial Infections - pathology</topic><topic>Humans</topic><topic>Microscopy, Confocal</topic><topic>Root Canal Filling Materials - pharmacology</topic><topic>sealer</topic><topic>sodium hypochlorite</topic><topic>Sodium Hypochlorite - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Tianfeng, PhD</creatorcontrib><creatorcontrib>Wang, Zhejun, PhD</creatorcontrib><creatorcontrib>Shen, Ya, PhD</creatorcontrib><creatorcontrib>Ma, Jingzhi, PhD</creatorcontrib><creatorcontrib>Cao, Yingguang, PhD</creatorcontrib><creatorcontrib>Haapasalo, Markus, Dr Odont</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Tianfeng, PhD</au><au>Wang, Zhejun, PhD</au><au>Shen, Ya, PhD</au><au>Ma, Jingzhi, PhD</au><au>Cao, Yingguang, PhD</au><au>Haapasalo, Markus, Dr Odont</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>41</volume><issue>8</issue><spage>1294</spage><epage>1298</epage><pages>1294-1298</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract Introduction The present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model. Methods Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion for 3 weeks. The biofilms in dentin were first subjected to 5% NaOCl or sterile water for 10 minutes followed by an equal thickness of AH Plus (Dentsply International Inc, York, PA), Endosequence BC Sealer (Brasseler USA, Savannah, GA), or MTA Fillapex (Angelus Indústria de Produtos Odontológicos S/A, Londrina, Brazil) placed on the root canal wall of the dentin specimens for 7, 30, and 60 days. Gutta-percha and water were used in a similar manner as controls. The proportions of dead and live bacteria inside the dentinal tubules were assessed by confocal laser scanning microscopy and viability staining. Results The combined use of NaOCl and sealers (30 and 60 days) killed significantly more bacteria than NaOCl or sealers alone ( P < .05). NaOCl + MTA Fillapex was the most effective antibacterial combination by killing 83% bacteria in dentin tubules in 60 days. Thirty and 60 days of exposure to the sealers resulted in significantly more dead bacteria in dentin biofilms than 7-day exposures ( P < .05). Conclusions The placement of root canal sealer after NaOCl treatment enhanced antibacterial effects against E. faecalis in the dentinal tubules. Little additional effect was obtained after 30 days of exposure to sealers.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26092772</pmid><doi>10.1016/j.joen.2015.04.023</doi><tpages>5</tpages></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Biofilms - drug effects Confocal laser scanning microscopy dentin Dentin - drug effects Dentin - microbiology Dentin - pathology Dentistry Drug Therapy, Combination Endocrinology & Metabolism Enterococcus faecalis Enterococcus faecalis - drug effects Epoxy Resins - pharmacology Gram-Positive Bacterial Infections - drug therapy Gram-Positive Bacterial Infections - pathology Humans Microscopy, Confocal Root Canal Filling Materials - pharmacology sealer sodium hypochlorite Sodium Hypochlorite - pharmacology Time Factors |
| title | Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals |
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