The Other Side of the RAAS: Aldosterone Improves Migration of Cardiac Progenitor Cells
Stem cell therapy is a promising new option for patients suffering from heart failure. Though many clinical studies show encouraging results, little is known about the signals which cause stem cells to home to diseased but not to healthy hearts. We hypothesized that aldosterone as one of the main pl...
Gespeichert in:
| Veröffentlicht in: | Journal of cellular physiology 2015-11, Vol.230 (11), p.2829-2836 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2836 |
|---|---|
| container_issue | 11 |
| container_start_page | 2829 |
| container_title | Journal of cellular physiology |
| container_volume | 230 |
| creator | Könemann, Stephanie Wenzel, Kristin Ameling, Sabine Grube, Karina Hammer, Elke Könemann, Raik Samal, Rasmita Völker, Uwe Felix, Stephan B. |
| description | Stem cell therapy is a promising new option for patients suffering from heart failure. Though many clinical studies show encouraging results, little is known about the signals which cause stem cells to home to diseased but not to healthy hearts. We hypothesized that aldosterone as one of the main players of heart failure functions as an attractant for progenitor cells and stimulates their migration. Stem cell antigen‐1 (Sca‐1) positive cells were isolated from the hearts of wild type FVB mice via magnetic cell sorting. The migration rate of the cells was determined using aldosterone as an attractant in a modified Boyden chamber (n = 5). Aldosterone led to a dose dependent increase in migration rate and this effect could be prevented by adding its blocker eplerenone. The mineralocorticoid receptor could be detected on Sca‐1+ cells via western blot and immunofluorescence. Therefore, aldosterone seems to play a role in stem cell migration and there the effect is most likely mediated by the mineralocorticoid receptor. J. Cell. Physiol. 9999: 2829–2836, 2015. © 2015 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/jcp.25013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1700106569</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1700106569</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4193-f0ee829abf8cc85e183cf672bcdad59b999498ec2b84d316c936b43491449e563</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhi0EokvhwB9AlrjQQ1qPvxJzW4VSCoVWdKFHy3EmbZZsvNhZoP8el217QILTaDTPvHqlh5DnwPaBMX6w9Ot9rhiIB2QGzJSF1Io_JLN8g8IoCTvkSUpLxpgxQjwmO1xVSgquZ-Tr4grp6XSFkZ73LdLQ0bzQz_P5-Ws6H9qQJoxhRHq8WsfwAxP92F9GN_VhvGFrF9veeXoWwyWO_RQirXEY0lPyqHNDwme3c5d8eXu4qN8VJ6dHx_X8pPASjCg6hlhx45qu8r5SCJXwnS5541vXKtMYY6Sp0POmkq0A7Y3QjRTSgJQGlRa75NU2N5f7vsE02VWffG7gRgybZKFkDJhW2mT05V_oMmzimNtZASAUSKXZ_yjQpjJlxTlkam9L-RhSitjZdexXLl5bYPZGic1K7B8lmX1xm7hpVtjek3cOMnCwBX72A17_O8m-r8_uIovtR5_t_Lr_cPGb1aUolb34dGQvFosP8IZrq8RvBZ-g-g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1698978221</pqid></control><display><type>article</type><title>The Other Side of the RAAS: Aldosterone Improves Migration of Cardiac Progenitor Cells</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Könemann, Stephanie ; Wenzel, Kristin ; Ameling, Sabine ; Grube, Karina ; Hammer, Elke ; Könemann, Raik ; Samal, Rasmita ; Völker, Uwe ; Felix, Stephan B.</creator><creatorcontrib>Könemann, Stephanie ; Wenzel, Kristin ; Ameling, Sabine ; Grube, Karina ; Hammer, Elke ; Könemann, Raik ; Samal, Rasmita ; Völker, Uwe ; Felix, Stephan B.</creatorcontrib><description>Stem cell therapy is a promising new option for patients suffering from heart failure. Though many clinical studies show encouraging results, little is known about the signals which cause stem cells to home to diseased but not to healthy hearts. We hypothesized that aldosterone as one of the main players of heart failure functions as an attractant for progenitor cells and stimulates their migration. Stem cell antigen‐1 (Sca‐1) positive cells were isolated from the hearts of wild type FVB mice via magnetic cell sorting. The migration rate of the cells was determined using aldosterone as an attractant in a modified Boyden chamber (n = 5). Aldosterone led to a dose dependent increase in migration rate and this effect could be prevented by adding its blocker eplerenone. The mineralocorticoid receptor could be detected on Sca‐1+ cells via western blot and immunofluorescence. Therefore, aldosterone seems to play a role in stem cell migration and there the effect is most likely mediated by the mineralocorticoid receptor. J. Cell. Physiol. 9999: 2829–2836, 2015. © 2015 Wiley Periodicals, Inc.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.25013</identifier><identifier>PMID: 25854326</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Aldosterone ; Aldosterone - administration & dosage ; Aldosterone - metabolism ; Animals ; Boyden chamber ; Cell migration ; Cell Movement - drug effects ; Cell therapy ; Cell- and Tissue-Based Therapy ; Congestive heart failure ; Coronary artery disease ; Gene Expression Regulation ; Heart diseases ; Heart failure ; Heart Failure - metabolism ; Heart Failure - therapy ; Heart rate ; Humans ; Immunofluorescence ; Mice ; Mineralocorticoid receptors ; Progenitor cells ; Receptors ; Receptors, Mineralocorticoid - biosynthesis ; Receptors, Mineralocorticoid - metabolism ; Spinocerebellar Ataxias - metabolism ; Stem Cell Transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - metabolism</subject><ispartof>Journal of cellular physiology, 2015-11, Vol.230 (11), p.2829-2836</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4193-f0ee829abf8cc85e183cf672bcdad59b999498ec2b84d316c936b43491449e563</citedby><cites>FETCH-LOGICAL-c4193-f0ee829abf8cc85e183cf672bcdad59b999498ec2b84d316c936b43491449e563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.25013$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.25013$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25854326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Könemann, Stephanie</creatorcontrib><creatorcontrib>Wenzel, Kristin</creatorcontrib><creatorcontrib>Ameling, Sabine</creatorcontrib><creatorcontrib>Grube, Karina</creatorcontrib><creatorcontrib>Hammer, Elke</creatorcontrib><creatorcontrib>Könemann, Raik</creatorcontrib><creatorcontrib>Samal, Rasmita</creatorcontrib><creatorcontrib>Völker, Uwe</creatorcontrib><creatorcontrib>Felix, Stephan B.</creatorcontrib><title>The Other Side of the RAAS: Aldosterone Improves Migration of Cardiac Progenitor Cells</title><title>Journal of cellular physiology</title><addtitle>J. Cell. Physiol</addtitle><description>Stem cell therapy is a promising new option for patients suffering from heart failure. Though many clinical studies show encouraging results, little is known about the signals which cause stem cells to home to diseased but not to healthy hearts. We hypothesized that aldosterone as one of the main players of heart failure functions as an attractant for progenitor cells and stimulates their migration. Stem cell antigen‐1 (Sca‐1) positive cells were isolated from the hearts of wild type FVB mice via magnetic cell sorting. The migration rate of the cells was determined using aldosterone as an attractant in a modified Boyden chamber (n = 5). Aldosterone led to a dose dependent increase in migration rate and this effect could be prevented by adding its blocker eplerenone. The mineralocorticoid receptor could be detected on Sca‐1+ cells via western blot and immunofluorescence. Therefore, aldosterone seems to play a role in stem cell migration and there the effect is most likely mediated by the mineralocorticoid receptor. J. Cell. Physiol. 9999: 2829–2836, 2015. © 2015 Wiley Periodicals, Inc.</description><subject>Aldosterone</subject><subject>Aldosterone - administration & dosage</subject><subject>Aldosterone - metabolism</subject><subject>Animals</subject><subject>Boyden chamber</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell therapy</subject><subject>Cell- and Tissue-Based Therapy</subject><subject>Congestive heart failure</subject><subject>Coronary artery disease</subject><subject>Gene Expression Regulation</subject><subject>Heart diseases</subject><subject>Heart failure</subject><subject>Heart Failure - metabolism</subject><subject>Heart Failure - therapy</subject><subject>Heart rate</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Mice</subject><subject>Mineralocorticoid receptors</subject><subject>Progenitor cells</subject><subject>Receptors</subject><subject>Receptors, Mineralocorticoid - biosynthesis</subject><subject>Receptors, Mineralocorticoid - metabolism</subject><subject>Spinocerebellar Ataxias - metabolism</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EokvhwB9AlrjQQ1qPvxJzW4VSCoVWdKFHy3EmbZZsvNhZoP8el217QILTaDTPvHqlh5DnwPaBMX6w9Ot9rhiIB2QGzJSF1Io_JLN8g8IoCTvkSUpLxpgxQjwmO1xVSgquZ-Tr4grp6XSFkZ73LdLQ0bzQz_P5-Ws6H9qQJoxhRHq8WsfwAxP92F9GN_VhvGFrF9veeXoWwyWO_RQirXEY0lPyqHNDwme3c5d8eXu4qN8VJ6dHx_X8pPASjCg6hlhx45qu8r5SCJXwnS5541vXKtMYY6Sp0POmkq0A7Y3QjRTSgJQGlRa75NU2N5f7vsE02VWffG7gRgybZKFkDJhW2mT05V_oMmzimNtZASAUSKXZ_yjQpjJlxTlkam9L-RhSitjZdexXLl5bYPZGic1K7B8lmX1xm7hpVtjek3cOMnCwBX72A17_O8m-r8_uIovtR5_t_Lr_cPGb1aUolb34dGQvFosP8IZrq8RvBZ-g-g</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>Könemann, Stephanie</creator><creator>Wenzel, Kristin</creator><creator>Ameling, Sabine</creator><creator>Grube, Karina</creator><creator>Hammer, Elke</creator><creator>Könemann, Raik</creator><creator>Samal, Rasmita</creator><creator>Völker, Uwe</creator><creator>Felix, Stephan B.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201511</creationdate><title>The Other Side of the RAAS: Aldosterone Improves Migration of Cardiac Progenitor Cells</title><author>Könemann, Stephanie ; Wenzel, Kristin ; Ameling, Sabine ; Grube, Karina ; Hammer, Elke ; Könemann, Raik ; Samal, Rasmita ; Völker, Uwe ; Felix, Stephan B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4193-f0ee829abf8cc85e183cf672bcdad59b999498ec2b84d316c936b43491449e563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aldosterone</topic><topic>Aldosterone - administration & dosage</topic><topic>Aldosterone - metabolism</topic><topic>Animals</topic><topic>Boyden chamber</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Cell therapy</topic><topic>Cell- and Tissue-Based Therapy</topic><topic>Congestive heart failure</topic><topic>Coronary artery disease</topic><topic>Gene Expression Regulation</topic><topic>Heart diseases</topic><topic>Heart failure</topic><topic>Heart Failure - metabolism</topic><topic>Heart Failure - therapy</topic><topic>Heart rate</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Mice</topic><topic>Mineralocorticoid receptors</topic><topic>Progenitor cells</topic><topic>Receptors</topic><topic>Receptors, Mineralocorticoid - biosynthesis</topic><topic>Receptors, Mineralocorticoid - metabolism</topic><topic>Spinocerebellar Ataxias - metabolism</topic><topic>Stem Cell Transplantation</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Könemann, Stephanie</creatorcontrib><creatorcontrib>Wenzel, Kristin</creatorcontrib><creatorcontrib>Ameling, Sabine</creatorcontrib><creatorcontrib>Grube, Karina</creatorcontrib><creatorcontrib>Hammer, Elke</creatorcontrib><creatorcontrib>Könemann, Raik</creatorcontrib><creatorcontrib>Samal, Rasmita</creatorcontrib><creatorcontrib>Völker, Uwe</creatorcontrib><creatorcontrib>Felix, Stephan B.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Könemann, Stephanie</au><au>Wenzel, Kristin</au><au>Ameling, Sabine</au><au>Grube, Karina</au><au>Hammer, Elke</au><au>Könemann, Raik</au><au>Samal, Rasmita</au><au>Völker, Uwe</au><au>Felix, Stephan B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Other Side of the RAAS: Aldosterone Improves Migration of Cardiac Progenitor Cells</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J. Cell. Physiol</addtitle><date>2015-11</date><risdate>2015</risdate><volume>230</volume><issue>11</issue><spage>2829</spage><epage>2836</epage><pages>2829-2836</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Stem cell therapy is a promising new option for patients suffering from heart failure. Though many clinical studies show encouraging results, little is known about the signals which cause stem cells to home to diseased but not to healthy hearts. We hypothesized that aldosterone as one of the main players of heart failure functions as an attractant for progenitor cells and stimulates their migration. Stem cell antigen‐1 (Sca‐1) positive cells were isolated from the hearts of wild type FVB mice via magnetic cell sorting. The migration rate of the cells was determined using aldosterone as an attractant in a modified Boyden chamber (n = 5). Aldosterone led to a dose dependent increase in migration rate and this effect could be prevented by adding its blocker eplerenone. The mineralocorticoid receptor could be detected on Sca‐1+ cells via western blot and immunofluorescence. Therefore, aldosterone seems to play a role in stem cell migration and there the effect is most likely mediated by the mineralocorticoid receptor. J. Cell. Physiol. 9999: 2829–2836, 2015. © 2015 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25854326</pmid><doi>10.1002/jcp.25013</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9541 |
| ispartof | Journal of cellular physiology, 2015-11, Vol.230 (11), p.2829-2836 |
| issn | 0021-9541 1097-4652 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1700106569 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Aldosterone Aldosterone - administration & dosage Aldosterone - metabolism Animals Boyden chamber Cell migration Cell Movement - drug effects Cell therapy Cell- and Tissue-Based Therapy Congestive heart failure Coronary artery disease Gene Expression Regulation Heart diseases Heart failure Heart Failure - metabolism Heart Failure - therapy Heart rate Humans Immunofluorescence Mice Mineralocorticoid receptors Progenitor cells Receptors Receptors, Mineralocorticoid - biosynthesis Receptors, Mineralocorticoid - metabolism Spinocerebellar Ataxias - metabolism Stem Cell Transplantation Stem cells Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism |
| title | The Other Side of the RAAS: Aldosterone Improves Migration of Cardiac Progenitor Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T04%3A20%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Other%20Side%20of%20the%20RAAS:%20Aldosterone%20Improves%20Migration%20of%20Cardiac%20Progenitor%20Cells&rft.jtitle=Journal%20of%20cellular%20physiology&rft.au=K%C3%B6nemann,%20Stephanie&rft.date=2015-11&rft.volume=230&rft.issue=11&rft.spage=2829&rft.epage=2836&rft.pages=2829-2836&rft.issn=0021-9541&rft.eissn=1097-4652&rft_id=info:doi/10.1002/jcp.25013&rft_dat=%3Cproquest_cross%3E1700106569%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1698978221&rft_id=info:pmid/25854326&rfr_iscdi=true |