Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma
Background and goals There are no highly sensitive and specific minimally invasive biomarkers for hepatocellular carcinoma (HCC) to date. The objective of this study was to identify serum microRNAs (miRNAs) as potential HCC biomarkers. Methods Using miRCURY LNA™ microRNA arrays, the levels of circul...
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| Veröffentlicht in: | Hepatology international 2013-03, Vol.7 (1), p.199-207 |
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| creator | Zhang, Zhoujing Ge, Shengxiang Wang, Xiaomin Yuan, Quan Yan, Qiang Ye, Huiming Che, Yaojian Lin, Yanyan Zhang, Jun Liu, Pingguo |
| description | Background and goals
There are no highly sensitive and specific minimally invasive biomarkers for hepatocellular carcinoma (HCC) to date. The objective of this study was to identify serum microRNAs (miRNAs) as potential HCC biomarkers.
Methods
Using miRCURY LNA™ microRNA arrays, the levels of circulating miRNAs in the serum of patients with HCC were compared and controls were matched. Then 253 subjects (112 HCC, 85 chronic hepatitis B [CHB], and 56 healthy controls) were recruited and 12 serum miRNAs were compared by quantitative real-time polymerase chain reaction (qRT-PCR). It was followed by the comparison of serum miRNA concentrations before and after the surgical resection in HCC group.
Results
Median levels of miR-483-5p and miR-500a were higher in HCC patients than in patients with CHB and in healthy controls (
p
0.05) and miR-483-5p levels were significantly reduced in serum samples obtained 30 days after surgical resection (
p
|
| doi_str_mv | 10.1007/s12072-012-9341-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1699489873</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2921052531</sourcerecordid><originalsourceid>FETCH-LOGICAL-c481t-6c12bf0ca3acc729d3ba1b8896a3b16cbc4fe232b0121caeed21fde7b07c4b723</originalsourceid><addsrcrecordid>eNp1kEtLxTAQhYMovn-AGwm4cRPNJG3SLkV8wQXBxzok6VSrfZm0C_315nJVRHA1A_PNmTOHkAPgJ8C5Po0guBaMg2ClzIB9rJFtKKViPM9g_aeXcovsxPjCeZ4rUJtkSyjBQclsmyzuMcwd7Zo7lhWS5SO1kVo6DhP2U2Nb6pqhs-EVA50GWuGEfqLPONpp8Ni2c2sD9Tb4pk_YHtmobRtx_6vuksfLi4fza7a4vbo5P1swnxUwMeVBuJp7K633WpSVdBZcUZTKSgfKO5_VKKRw6THwFrESUFeoHdc-c1rIXXK80h3D8DZjnEzXxKUd2-MwRwOqLLOiLLRM6NEf9GWYQ5_cGZCgNYdS54mCFeXDEGPA2oyhSW-_G-BmGbVZRW2SI7OM2nykncMv5dl1WP1sfGebALECYhr1Txh-nf5X9RMma4kE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1317701975</pqid></control><display><type>article</type><title>Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma</title><source>SpringerLink Journals - AutoHoldings</source><creator>Zhang, Zhoujing ; Ge, Shengxiang ; Wang, Xiaomin ; Yuan, Quan ; Yan, Qiang ; Ye, Huiming ; Che, Yaojian ; Lin, Yanyan ; Zhang, Jun ; Liu, Pingguo</creator><creatorcontrib>Zhang, Zhoujing ; Ge, Shengxiang ; Wang, Xiaomin ; Yuan, Quan ; Yan, Qiang ; Ye, Huiming ; Che, Yaojian ; Lin, Yanyan ; Zhang, Jun ; Liu, Pingguo</creatorcontrib><description>Background and goals
There are no highly sensitive and specific minimally invasive biomarkers for hepatocellular carcinoma (HCC) to date. The objective of this study was to identify serum microRNAs (miRNAs) as potential HCC biomarkers.
Methods
Using miRCURY LNA™ microRNA arrays, the levels of circulating miRNAs in the serum of patients with HCC were compared and controls were matched. Then 253 subjects (112 HCC, 85 chronic hepatitis B [CHB], and 56 healthy controls) were recruited and 12 serum miRNAs were compared by quantitative real-time polymerase chain reaction (qRT-PCR). It was followed by the comparison of serum miRNA concentrations before and after the surgical resection in HCC group.
Results
Median levels of miR-483-5p and miR-500a were higher in HCC patients than in patients with CHB and in healthy controls (
p
< 0.0001), but there were no differences between CHB patients and healthy controls (
p
> 0.05) and miR-483-5p levels were significantly reduced in serum samples obtained 30 days after surgical resection (
p
< 0.0001). The area under receiver operating characteristic curves of miR-483-5p and miR-500a was 74% (cutoff [Ct] value = 2.824, sensitivity = 74%, and specificity = 66%) and 66% (Ct value = 1.830, sensitivity = 74%, and specificity = 51%) for the prediction of HCC, respectively. In detecting HCC, combining
α
-fetoprotein (AFP) and serum miR-483-5p (sensitivity = 81% and specificity = 83%) was better than AFP alone (sensitivity = 78%, specificity = 70%).
Conclusion
Our observations suggest that serum miR-483-5p and miR-500a might serve as novel, noninvasive biomarkers for HCC. Serum miR-483-5p might complement AFP in detecting HCC.</description><identifier>ISSN: 1936-0533</identifier><identifier>EISSN: 1936-0541</identifier><identifier>DOI: 10.1007/s12072-012-9341-z</identifier><identifier>PMID: 26201634</identifier><language>eng</language><publisher>India: Springer-Verlag</publisher><subject>Colorectal Surgery ; Hepatology ; Medicine ; Medicine & Public Health ; Original Article ; Surgery</subject><ispartof>Hepatology international, 2013-03, Vol.7 (1), p.199-207</ispartof><rights>Asian Pacific Association for the Study of the Liver 2012</rights><rights>Asian Pacific Association for the Study of the Liver 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-6c12bf0ca3acc729d3ba1b8896a3b16cbc4fe232b0121caeed21fde7b07c4b723</citedby><cites>FETCH-LOGICAL-c481t-6c12bf0ca3acc729d3ba1b8896a3b16cbc4fe232b0121caeed21fde7b07c4b723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12072-012-9341-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12072-012-9341-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26201634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Zhoujing</creatorcontrib><creatorcontrib>Ge, Shengxiang</creatorcontrib><creatorcontrib>Wang, Xiaomin</creatorcontrib><creatorcontrib>Yuan, Quan</creatorcontrib><creatorcontrib>Yan, Qiang</creatorcontrib><creatorcontrib>Ye, Huiming</creatorcontrib><creatorcontrib>Che, Yaojian</creatorcontrib><creatorcontrib>Lin, Yanyan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Liu, Pingguo</creatorcontrib><title>Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma</title><title>Hepatology international</title><addtitle>Hepatol Int</addtitle><addtitle>Hepatol Int</addtitle><description>Background and goals
There are no highly sensitive and specific minimally invasive biomarkers for hepatocellular carcinoma (HCC) to date. The objective of this study was to identify serum microRNAs (miRNAs) as potential HCC biomarkers.
Methods
Using miRCURY LNA™ microRNA arrays, the levels of circulating miRNAs in the serum of patients with HCC were compared and controls were matched. Then 253 subjects (112 HCC, 85 chronic hepatitis B [CHB], and 56 healthy controls) were recruited and 12 serum miRNAs were compared by quantitative real-time polymerase chain reaction (qRT-PCR). It was followed by the comparison of serum miRNA concentrations before and after the surgical resection in HCC group.
Results
Median levels of miR-483-5p and miR-500a were higher in HCC patients than in patients with CHB and in healthy controls (
p
< 0.0001), but there were no differences between CHB patients and healthy controls (
p
> 0.05) and miR-483-5p levels were significantly reduced in serum samples obtained 30 days after surgical resection (
p
< 0.0001). The area under receiver operating characteristic curves of miR-483-5p and miR-500a was 74% (cutoff [Ct] value = 2.824, sensitivity = 74%, and specificity = 66%) and 66% (Ct value = 1.830, sensitivity = 74%, and specificity = 51%) for the prediction of HCC, respectively. In detecting HCC, combining
α
-fetoprotein (AFP) and serum miR-483-5p (sensitivity = 81% and specificity = 83%) was better than AFP alone (sensitivity = 78%, specificity = 70%).
Conclusion
Our observations suggest that serum miR-483-5p and miR-500a might serve as novel, noninvasive biomarkers for HCC. Serum miR-483-5p might complement AFP in detecting HCC.</description><subject>Colorectal Surgery</subject><subject>Hepatology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><subject>Surgery</subject><issn>1936-0533</issn><issn>1936-0541</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kEtLxTAQhYMovn-AGwm4cRPNJG3SLkV8wQXBxzok6VSrfZm0C_315nJVRHA1A_PNmTOHkAPgJ8C5Po0guBaMg2ClzIB9rJFtKKViPM9g_aeXcovsxPjCeZ4rUJtkSyjBQclsmyzuMcwd7Zo7lhWS5SO1kVo6DhP2U2Nb6pqhs-EVA50GWuGEfqLPONpp8Ni2c2sD9Tb4pk_YHtmobRtx_6vuksfLi4fza7a4vbo5P1swnxUwMeVBuJp7K633WpSVdBZcUZTKSgfKO5_VKKRw6THwFrESUFeoHdc-c1rIXXK80h3D8DZjnEzXxKUd2-MwRwOqLLOiLLRM6NEf9GWYQ5_cGZCgNYdS54mCFeXDEGPA2oyhSW-_G-BmGbVZRW2SI7OM2nykncMv5dl1WP1sfGebALECYhr1Txh-nf5X9RMma4kE</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Zhang, Zhoujing</creator><creator>Ge, Shengxiang</creator><creator>Wang, Xiaomin</creator><creator>Yuan, Quan</creator><creator>Yan, Qiang</creator><creator>Ye, Huiming</creator><creator>Che, Yaojian</creator><creator>Lin, Yanyan</creator><creator>Zhang, Jun</creator><creator>Liu, Pingguo</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma</title><author>Zhang, Zhoujing ; Ge, Shengxiang ; Wang, Xiaomin ; Yuan, Quan ; Yan, Qiang ; Ye, Huiming ; Che, Yaojian ; Lin, Yanyan ; Zhang, Jun ; Liu, Pingguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-6c12bf0ca3acc729d3ba1b8896a3b16cbc4fe232b0121caeed21fde7b07c4b723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Colorectal Surgery</topic><topic>Hepatology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zhoujing</creatorcontrib><creatorcontrib>Ge, Shengxiang</creatorcontrib><creatorcontrib>Wang, Xiaomin</creatorcontrib><creatorcontrib>Yuan, Quan</creatorcontrib><creatorcontrib>Yan, Qiang</creatorcontrib><creatorcontrib>Ye, Huiming</creatorcontrib><creatorcontrib>Che, Yaojian</creatorcontrib><creatorcontrib>Lin, Yanyan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Liu, Pingguo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Hepatology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhoujing</au><au>Ge, Shengxiang</au><au>Wang, Xiaomin</au><au>Yuan, Quan</au><au>Yan, Qiang</au><au>Ye, Huiming</au><au>Che, Yaojian</au><au>Lin, Yanyan</au><au>Zhang, Jun</au><au>Liu, Pingguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma</atitle><jtitle>Hepatology international</jtitle><stitle>Hepatol Int</stitle><addtitle>Hepatol Int</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>7</volume><issue>1</issue><spage>199</spage><epage>207</epage><pages>199-207</pages><issn>1936-0533</issn><eissn>1936-0541</eissn><abstract>Background and goals
There are no highly sensitive and specific minimally invasive biomarkers for hepatocellular carcinoma (HCC) to date. The objective of this study was to identify serum microRNAs (miRNAs) as potential HCC biomarkers.
Methods
Using miRCURY LNA™ microRNA arrays, the levels of circulating miRNAs in the serum of patients with HCC were compared and controls were matched. Then 253 subjects (112 HCC, 85 chronic hepatitis B [CHB], and 56 healthy controls) were recruited and 12 serum miRNAs were compared by quantitative real-time polymerase chain reaction (qRT-PCR). It was followed by the comparison of serum miRNA concentrations before and after the surgical resection in HCC group.
Results
Median levels of miR-483-5p and miR-500a were higher in HCC patients than in patients with CHB and in healthy controls (
p
< 0.0001), but there were no differences between CHB patients and healthy controls (
p
> 0.05) and miR-483-5p levels were significantly reduced in serum samples obtained 30 days after surgical resection (
p
< 0.0001). The area under receiver operating characteristic curves of miR-483-5p and miR-500a was 74% (cutoff [Ct] value = 2.824, sensitivity = 74%, and specificity = 66%) and 66% (Ct value = 1.830, sensitivity = 74%, and specificity = 51%) for the prediction of HCC, respectively. In detecting HCC, combining
α
-fetoprotein (AFP) and serum miR-483-5p (sensitivity = 81% and specificity = 83%) was better than AFP alone (sensitivity = 78%, specificity = 70%).
Conclusion
Our observations suggest that serum miR-483-5p and miR-500a might serve as novel, noninvasive biomarkers for HCC. Serum miR-483-5p might complement AFP in detecting HCC.</abstract><cop>India</cop><pub>Springer-Verlag</pub><pmid>26201634</pmid><doi>10.1007/s12072-012-9341-z</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Hepatology international, 2013-03, Vol.7 (1), p.199-207 |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Colorectal Surgery Hepatology Medicine Medicine & Public Health Original Article Surgery |
| title | Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma |
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